Skip to main content
Log in

Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67

  • Full Articles
  • Published:
Russian Chemical Bulletin Aims and scope

Abstract

Capsular polysaccharide (CPS) was isolated from a nosocomial pathogen Acinetobacter baumannii (A. baumannii) NIPH67 and studied by sugar analysis, Smith degradation, and 1H and 13C NMR spectroscopy. The CPS was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Pse5Ac7Ac), and the structure of the linear trisaccharide repeating unit of the CPS was established as →4)-α-Psep5Ac7Ac-(2→6)-β-d-Galp-(1→3)-α-d-GalpNAc-(1→. The genetic content of the capsule biosynthesis cluster of A. baumannii NIPH67, designated KL33, is consistent with the established CPS structure, and thus the capsule of the investigated strain was assigned to K33 group. Functions of proteins including two glycosyltransferases encoded by the genes of the K33 locus were assigned based on the structure of CPS and by the comparison with related proteins of other capsular types of A. baumannii.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. T. A. Russo, N. R. Luke, J. M. Beanan, R. Olson, S. L. Sauberan, U. MacDonald, L. W. Schultz, T. C. Umland, A. A. Campagnari, Infect. Immun., 2010, 78, 3993.

    Article  CAS  Google Scholar 

  2. J. J. Kenyon, R. M. Hall, PLoS ONE, 2013, 8, e62160.

    Article  CAS  Google Scholar 

  3. D. Hu, B. Liu, L. Dijkshoorn, L. Wang, P. R. Reeves, PLoS ONE, 2013, 8, e70329.

    Article  CAS  Google Scholar 

  4. O. Westphal, K. Jann, Methods Carbohydr. Chem., 1965, 5, 83.

    CAS  Google Scholar 

  5. J. J. Kenyon, S. J. Nigro, R. M. Hall, PLoS ONE, 2014, 9, e107833.

    Article  Google Scholar 

  6. C. Galanos, O. Lüderitz, O. Westphal, Eur. J. Biochem., 1969, 9, 245.

    Article  CAS  Google Scholar 

  7. G. M. Lipkind, A. S. Shashkov, Y. A. Knirel, E. V. Vinogradov, N. K. Kochetkov, Carbohydr. Res., 1988, 175, 59.

    Article  CAS  Google Scholar 

  8. Y. A. Knirel, A. S. Shashkov, Y. E. Tsvetkov, P.-E. Jansson, U. Zähringer, Adv. Carbohydr. Chem. Biochem., 2003, 58, 371.

    Article  CAS  Google Scholar 

  9. P.-E. Jansson, L. Kenne, G. Widmalm, Carbohydr. Res., 1989, 188, 169.

    Article  CAS  Google Scholar 

  10. S. N. Senchenkova, A. S. Shashkov, M. M. Shneider, N. P. Arbatsky, A. V. Popova, K. A. Miroshnikov, N. V. Volozhantsev, Y. A. Knirel, Carbohydr. Res., 2014, 391, 89.

    Article  CAS  Google Scholar 

  11. J. J. Kenyon, A. M. Marzaioli, R. M. Hall, C. De Castro, Glycobiology, 2014, 24, 554.

    Article  CAS  Google Scholar 

  12. S. N. Senchenkova, A. V. Popova, A. S. Shashkov, M. M. Shneider, Z. Mei, N. P. Arbatsky, B. Liu, K. A. Miroshnikov, N. V. Volozhantsev, Y. A. Knirel, Carbohydr. Res., 2015, 407, 154.

    Article  CAS  Google Scholar 

  13. J. J. Kenyon, A. M. Marzaioli, R. M. Hall, C. De Castro, Carbohydr. Res., 2015, 409, 30.

    Article  CAS  Google Scholar 

  14. B. Périchon, S. Goussard, V. Walewski, L. Krizova, G. Cerqueira, C. Murphy, M. Feldgarden, J. Wortman, D. Clermont, A. Nemec, P. Courvalin, Antimicrob. Agents Chemother., 2014, 58, 936.

    Article  Google Scholar 

  15. R. G. Lees-Miller, J. A. Iwashkiw, N. E. Scott, A. Seper, E. Vinogradov, S. Schild, M. F. Feldman, Mol. Microbiol., 2013, 89, 816.

    Article  CAS  Google Scholar 

  16. L. M. Willis, C. Whitfield, Proc. Natl. Acad. Sci. USA, 2013, 110, 20753.

    Article  CAS  Google Scholar 

  17. J. J. Kenyon, A. M. Marzaioli, C. De Castro, R. M. Hall, Glycobiology, 2015, 25, 644.

    Article  CAS  Google Scholar 

  18. M. Touchon, J. Cury, E.-J. Yoon, L. Krizova, G. C. Cerqueira, C. Murphy, M. Feldgarden, J. Wortman, D. Clermont, T. Thierry Lambert, C. Grillot-Courvalin, A. Nemec, P. Courvalin, E. P. C. Rocha, Genome Biol. Evol., 2014, 6, 2866.

    Article  CAS  Google Scholar 

  19. A. S. Shashkov, G. M. Lipkind, Y. A. Knirel, N. K. Kochetkov, Magn. Reson. Chem., 1988, 26, 735.

    Article  CAS  Google Scholar 

  20. S. N. Senchenkova, A. S. Shashkov, A. V. Popova, M. M. Shneider, N. P. Arbatsky, K. A. Miroshnikov, N. V. Volozhantsev, Y. A. Knirel, Carbohydr. Res., 2015, 408, 8.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Y. A. Knirel.

Additional information

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 2, pp. 0588—0591, February, 2016.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Arbatsky, N.P., Shneider, M.M., Shashkov, A.S. et al. Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67. Russ Chem Bull 65, 588–591 (2016). https://doi.org/10.1007/s11172-016-1342-y

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11172-016-1342-y

Key words

Navigation