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DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for taxonomic identification of Sideritis species growing in Greece

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Abstract

Identification of genotypes in Sideritis is complicated owing to the morphological similarity and common occurrence of natural hybridisation within Sideritis species. Species- and genotype-specific DNA markers are very useful for plant identification, breeding and preservation programs. Herein, a real-time polymerase chain reaction (PCR) of ITS2 barcode region coupled with high resolution melting-curve (HRM) analysis was evaluated for an accurate, rapid and sensitive tool for species identification focusing on seven Sideritis species growing in Greece. The HRM assay developed in this study is a rapid and straightforward method for the identification and discrimination of the investigated Sideritis species. This assay is simple compared to other genotyping methods as it does not require DNA sequencing or post-PCR processing. Therefore, this method offers a new alternative for rapid detection of Sideritis species.

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Acknowledgments

Part of this study was funded by the SEE-ERA.NET PLUS-ERA 135/01 MOUNTEA-CONSE Project. The authors would like to thank Prof Dr S. Kokkini for the valuable discussions on a sample of Sideritis.

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Authors and Affiliations

  1. Institute of Applied Biosciences, CERTH, Thermi, 570 01, Thessaloniki, Greece

    Ioannis Ganopoulos, Aliki Xanthopoulou, Athanasios Tsaftaris & Panagiotis Madesis

  2. Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, 54 124, Thessaloniki, Greece

    Ioannis Ganopoulos, Aliki Xanthopoulou & Athanasios Tsaftaris

  3. Cotton and Industrial Plants Institute, Hellenic Agricultural Organization “Demeter”, Thermi, 57001, Thessaloniki, Greece

    Apostolos Kalivas

  4. Department of Aromatic and Medicinal Plants, Agricultural Research Centre of Northern Greece, N.AG.RE.F., Thermi, Thessaloniki, Greece

    Paschalina Chatzopoulou

Authors
  1. Apostolos Kalivas
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  2. Ioannis Ganopoulos
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  3. Aliki Xanthopoulou
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  4. Paschalina Chatzopoulou
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  5. Athanasios Tsaftaris
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  6. Panagiotis Madesis
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Correspondence to Panagiotis Madesis.

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Kalivas, A., Ganopoulos, I., Xanthopoulou, A. et al. DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for taxonomic identification of Sideritis species growing in Greece. Mol Biol Rep 41, 5147–5155 (2014). https://doi.org/10.1007/s11033-014-3381-5

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  • DOI: https://doi.org/10.1007/s11033-014-3381-5

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