References
Hammond Jr J. Recovery and culture of tubal mouse ova. Nature. 1949;163:28.
Whitten WK. Culture of tubal mouse ova. Nature. 1956;177:96.
McLaren A, Biggers JD. Successful development and birth of mice cultivated in vitro as early embryos. Nature. 1958;182:877–8.
Biggers JD. Pioneering mammalian embryo culture. In: Bavister BD, editor. The mammalian preimplantation embryo. New York: Plenum Publishing Corporation; 1987. p. 1–22.
Biggers JD. The culture of the mammalian preimplantation embryo. In: Gianaroli L, Campana A, Trounson AO, editors. Implantation in mammals. New York: Serono Symposia Publications from Raven; 1993.
Leese HJ. Human embryo culture: back to nature. J Assist Reprod Genet. 1998;15:466–8.
Biggers JD. Fundamentals of the design of culture media that supports human preimplantation development. In: Van Blerkom J, editor. Essential IVF. Norwell: Klewer Adademic Press; 2003. p. 291–332.
Summers MC, Biggers JD. Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues. Hum Reprod Update. 2003;9:557–82.
Biggers JD, Rinaldini LM, Webb M. The study of growth factors in tissue culture. Symp Soc Exp Biol. 1957;11:264–97.
Biggers JD, Whitten WK, Whittingham DG. The culture of mouse embryos in vitro. In: Daniel JC, editor. Methods in mammalian embryology. San Francisco: Freeman Press; 1971. p. 86–116.
Lawitts JA, Biggers JD. Optimization of mouse embryo culture media using simplex methods. J Reprod Fertil. 1991;91:543–56.
Lawitts J, Biggers JD. Culture of preimplantation embryos. 1993.
Erbach GT, Lawitts JA, Papaioannou VE, Biggers JD. Differential growth of the mouse preimplantation embryo in chemically defined media. Biol Reprod. 1994;50:1027–33.
Ho Y, Wigglesworth K, Eppig JJ, Schultz RM. Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. Mol Reprod Dev. 1995;41:232–8.
Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–7.
Biggers JD, McGinnis LK, Raffin M. Amino acids and preimplantation development of the mouse in protein-free potassium simplex optimized medium. Biol Reprod. 2000;63:281–93.
Biggers JD, McGinnis LK, Summers MC. Discrepancies between the effects of glutamine in cultures of preimplantation mouse embryos. Reprod Biomed Online. 2004;9:70–3.
Biggers JD, McGinnis LK, Lawitts JA. Enhanced effect of glycyl-L-glutamine on mouse preimplantation embryos in vitro. Reprod Biomed Online. 2004;9:59–69.
Summers MC, Bhatnagar PR, Lawitts JA, Biggers JD. Fertilization in vitro of mouse ova from inbred and outbred strains: complete preimplantation embryo development in glucose-supplemented KSOM. Biol Reprod. 1995;53:431–7.
Lawitts JA, Biggers JD. Joint effects of sodium chloride, glutamine, and glucose in mouse preimplantation embryo culture media. Mol Reprod Dev. 1992;31:189–94.
Biggers JD, McGinnis LK. Evidence that glucose is not always an inhibitor of mouse preimplantation development in vitro. Hum Reprod. 2001;16:153–63.
Summers MC, McGinnis LK, Lawitts JA, Raffin M, Biggers JD. IVF of mouse ova in a simplex optimized medium supplemented with amino acids. Hum Reprod. 2000;15:1791–801.
Summers MC, McGinnis LK, Lawitts JA, Biggers JD. Mouse embryo development following IVF in media containing either L-glutamine or glycyl-L-glutamine. Hum Reprod. 2005;20:1364–71.
Gardner DK. Development of serum-free media for the culture and transfer of human blastocysts. Hum Reprod. 1998;13 Suppl 4:218–25.
Biggers JD, Summers MC. Choosing a culture medium: making informed choices. Fertil Steril. 2008;90:473–83.
Biggers JD, Racowsky C. The development of fertilized human ova to the blastocyst stage in KSOM(AA) medium: is a two-step protocol necessary? Reprod Biomed Online. 2002;5:133–40.
Macklon NS, Pieters MH, Hassan MA, Jeucken PH, Eijkemans MJ, Fauser BC. A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development. Hum Reprod. 2002;17:2700–5.
Biggers JD, Summers MC. Impact of hyperglycemia on early embryo development and embryopathy: in vitro experiments using a mouse model. Hum Reprod. 2008;23:2874–5.
Biggers JD, McGinnis LK, Lawitts JA. One-step versus two-step culture of mouse preimplantation embryos: is there a difference? Hum Reprod. 2005;20:3376–84.
Reed ML, Hamic A, Thompson DJ, Caperton CL. Continuous uninterrupted single medium culture without medium renewal versus sequential media culture: a sibling embryo study. Fertil Steril. 2009;92:1783–6.
Sepulveda S, Garcia J, Arriaga E, Diaz J, Noriega-Portella L, Noriega-Hoces L. In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system. Fertil Steril. 2009;91:1765–70.
Carrilo AJ, Yalcinkaya TM. Improved embryo development and comparable pregnancy rates using single vs. sequential culture media from the day of oocyte retrieval to the blastocyst stage of development. J Clin Embryol. 2010;13:35–42.
Summers MC, Bird S, Mirzai FM, Thornhill A, Biggers JD. Human preimplantation embryo development in vitro: a morphological assessment of sibling zygotes cultured in a single medium or in sequential media. Hum Fertil (Camb) 2013.
Doherty AS, Mann MR, Tremblay KD, Bartolomei MS, Schultz RM. Differential effects of culture on imprinted H19 expression in the preimplantation mouse embryo. Biol Reprod. 2000;62:1526–35.
Rinaudo P, Schultz RM. Effects of embryo culture on global pattern of gene expression in preimplantation mouse embryos. Reproduction. 2004;128:301–11.
Johnson MH. The problematic in-vitro embryo in the age of epigenetics. Reprod Biomed Online. 2005;10:88–96.
McElrath TF, Wise PH. Fertility therapy and the risk of very low birth weight. Obstet Gynecol. 1997;90:600–5.
Romundstad LB, Romundstad PR, Sunde A, von During V, Skjaerven R, Gunnell D, et al. Effects of technology or maternal factors on perinatal outcome after assisted fertilisation: a population-based cohort study. Lancet. 2008;372:737–43.
DeBaun MR, Niemitz EL, Feinberg AP. Association of in vitro fertilization with Beckwith-Wiedemann syndrome and epigenetic alterations of LIT1 and H19. Am J Hum Genet. 2003;72:156–60.
Gicquel C, Gaston V, Mandelbaum J, Siffroi JP, Flahault A, Le Bouc Y. In vitro fertilization may increase the risk of Beckwith-Wiedemann syndrome related to the abnormal imprinting of the KCN1OT gene. Am J Hum Genet. 2003;72:1338–41.
Ludwig M, Katalinic A, Gross S, Sutcliffe A, Varon R, Horsthemke B. Increased prevalence of imprinting defects in patients with Angelman syndrome born to subfertile couples. J Med Genet. 2005;42:289–91.
Dumoulin JC, Land JA, Van Montfoort AP, Nelissen EC, Coonen E, Derhaag JG, et al. Effect of in vitro culture of human embryos on birthweight of newborns. Hum Reprod. 2010;25:605–12.
Nelissen EC, Van Montfoort AP, Coonen E, Derhaag JG, Geraedts JP, Smits LJ, et al. Further evidence that culture media affect perinatal outcome: findings after transfer of fresh and cryopreserved embryos. Hum Reprod. 2012;27:1966–76.
Eaton JL, Lieberman ES, Stearns C, Chinchilla M, Racowsky C. Embryo culture media and neonatal birthweight following IVF. Hum Reprod. 2012;27:375–9.
Vergouw CG, Kostelijk EH, Doejaaren E, Hompes PG, Lambalk CB, Schats R. The influence of the type of embryo culture medium on neonatal birthweight after single embryo transfer in IVF. Hum Reprod. 2012;27:2619–26.
Lin S, Li M, Lian Y, Chen L, Liu P. No effect of embryo culture media on birthweight and length of newborns. Hum Reprod. 2013;28:1762–7.
Biggers JD. Ethical issues and the commercialization of embryo culture media. Reprod Biomed Online. 2000;1:74–6.
Chatot CL, Ziomek CA, Bavister BD, Lewis JL, Torres I. An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J Reprod Fertil. 1989;86:679–88.
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Capsule In honoring Professor Biggers on the ocassion of his 90th birthday, this article reviews his seminal contributions towards the development of KSOM media.
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Summers, M.C. A brief history of the development of the KSOM family of media. J Assist Reprod Genet 30, 995–999 (2013). https://doi.org/10.1007/s10815-013-0097-8
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DOI: https://doi.org/10.1007/s10815-013-0097-8