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Characterization of the psychrotolerant acetogen strain SyrA5 and the emended description of the species Acetobacterium carbinolicum

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Abstract

A psychrotolerant, obligate anaerobic, acetogenic bacterium designated strain SyrA5 was isolated from black anoxic sediment of a brackish fjord. Cells were Gram-positive, non-sporeforming rods. The isolate utilized H2/CO2, CO, fructose, glucose, ethanol, ethylene glycol, glycerol, pyruvate, lactate, betaine and the methyl-groups of several methoxylated benzoic derivatives such as syringate, trimethoxybenzoate and vallinate. The optimum temperature for growth was 29 °C, whilst slow growth occurred at 2 °C. The strain grew optimally with NaCl concentrations below 2.7% (w/v), but growth occurred up to 4.3% (w/v) NaCl. Growth was observed in the range from pH 5.9 to 8.5, optimum at pH 8. The G+C content was 44.1 mol%. Based upon 16S rRNA gene sequence analysis and DNA–DNA reassociation studies, the organism was classified in the genus Acetobacterium. Strain SyrA5 shared a 16S rRNA sequence similarity with A. carbinolicum of 100%, a fthfs gene (which codes for the N5,N10 tetrahydrofolate synthetase) sequence identity of 98.5–98.7% (amino acid sequence similarities were 99.4–100%) and a RNA–DNA hybridization homology of 64–68%. Despite a number of phenotypic differences between strain SyrA5 and A. carbinolicum we propose including strain SyrA5 as a subspecies of A. carbinolicum for which we propose the name Acetobacterium carbinolicum subspecies kysingense. The type strain is SyrA5 (=DSM 16427T, ATCC BAA-990).

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Acknowledgements

The study was supported by NSF grant No. 21-00-0309. The authors thank Peter Westermann for providing help with the measurement of acetate.

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  1. Department of Microbiology, Bldg. 540, Institute of Biological Sciences, University of Aarhus, Dk-8000, Aarhus C, Denmark

    Maiken Paarup & Kai Finster

  2. Max-Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse, D-35043, Marbug, Denmark

    Michael W. Friedrich

  3. DSMZ GmbH, Mascheroder Weg 1b, D-38124, Braunschweig, Germany

    Brian J. Tindall

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  1. Maiken Paarup
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Correspondence to Kai Finster.

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Paarup, M., Friedrich, M., Tindall, B. et al. Characterization of the psychrotolerant acetogen strain SyrA5 and the emended description of the species Acetobacterium carbinolicum . Antonie Van Leeuwenhoek 89, 55–69 (2006). https://doi.org/10.1007/s10482-005-9009-y

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