Skip to main content
Log in

Complete nucleotide sequence and latency of a novel blueberry-infecting closterovirus

  • Viral and Viroid Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

A novel latent closterovirus was detected from highbush blueberry in Japan and provisionally named blueberry virus A (BVA). The BVA genome (17,798 nucleotides) contains 10 open reading frames, but no minor coat protein could be identified in the virus genome. The BVA RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homolog (HSP70h), and major coat protein (CP) shared the highest amino acid sequence identities with those of viruses in the genus Closterovirus (61.2, 27.6, and 20.9 %, respectively). In a phylogenetic analysis of the RdRp, HSP70h, and CP, BVA did not cluster with any genus in the family Closteroviridae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

References

  • Agranovski AA, Koonin EV, Boyko VP, Maiss E, Frötschl R, Lunina NA, Atabekov JG (1994) Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease. Virology 198:311–324

    Article  Google Scholar 

  • Agranovsky AA, Lesemann DE, Maiss E, Hull R, Atabekov JG (1995) “Rattlesnake” structure of a filamentous plant RNA virus built of two capsid proteins. Proc Natl Acad Sci USA 92:2470–2473

    Article  PubMed  CAS  Google Scholar 

  • Bork P, Sander C, Valencia A (1992) An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins. Proc Natl Acad Sci USA 89:7290–7294

    Article  PubMed  CAS  Google Scholar 

  • Candresse T, Morch MD, Dunez J (1990) Multiple alignment and hierarchical clustering of conserved amino acid sequences in the replication-associated proteins of plant RNAviruses. Res Virol 141:315–329

    Article  PubMed  CAS  Google Scholar 

  • Caruso FL, Ramsdell DC (1995) Compendium of blueberry and cranberry disease. Disease compendium series. APS Press, St. Paul

    Google Scholar 

  • Dolja VV, Boyko VP, Agranovsky AA, Koonin EV (1991) Phylogeny of capsid proteins of rod-shaped and filamentous RNA plant viruses: two families with distinct patterns of sequence and probably structure conservation. Virology 184:79–86

    Article  PubMed  CAS  Google Scholar 

  • Dolja VV, Kreuze JF, Valkonen JPT (2006) Comparative and functional genomics of closteroviruses. Virus Res 117:38–51

    Article  PubMed  CAS  Google Scholar 

  • Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy. In: 8th report of the international committee on taxonomy of viruses. Elsevier Academic Press, Amsterdam, pp 1077–1087

  • Isogai M, Ishii K, Umemoto S, Watanabe M, Yoshikawa N (2009) First report of blueberry red ringspot disease caused by blueberry red ringspot virus in Japan. J Gen Plant Pathol 75:140–143

    Article  Google Scholar 

  • Isogai M, Nakamura T, Ishii K, Watanabe M, Yoshikawa N (2011) Histochemical detection of blueberry latent virus in highbush blueberry plant. J Gen Plant Pathol 77:304–306

    Article  CAS  Google Scholar 

  • Isogai M, Nakamura T, Ujiie C, Watanabe M, Yoshikawa N (2012) Identification and characterization of blueberry latent spherical virus, a new member of subgroup C in the genus Nepovirus. Arch Virol 157:297–303

    Article  PubMed  CAS  Google Scholar 

  • Jagadish MN, Huang D, Ward CW (1993) Site-directed mutagenesis of a potyvirus coat protein and its assembly in Escherichia coli. J Gen Virol 74:893–896

    Article  PubMed  CAS  Google Scholar 

  • Karasev AV (2000) Genetic diversity and evolution of closteroviruses. Annu Rev Phytopathol 38:293–324

    Article  PubMed  CAS  Google Scholar 

  • Koonin EV (1991) The phylogeny of RNA dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol 72:2197–2206

    Article  PubMed  Google Scholar 

  • Maliogka VI, Dovas CI, Lotos L, Efthimiou K, Katis NI (2009) Complete genome analysis and immunodetection of a member of a novel virus species belonging to the genus Ampelovirus. Arch Virol 154:209–218

    Article  PubMed  CAS  Google Scholar 

  • Martelli GP, Agranovsky AA, Bar-Joseph M, Boscia D, Candresse T, Coutts RHA, Dolja VV, Falk BW, Gonsalves D, Jelkmann W, Karasev AV, Minafra A, Namba S, Vetten HJ, Wisler GC, Yoshikawa N (2002) The family Closteroviridae revised. Arch Virol 147:2039–2044

    Article  PubMed  CAS  Google Scholar 

  • Martin RR, Zhou J, Tzanetakis IE (2011) Blueberry latent virus: an amalgam of the Partitiviridae and Totiviridae. Virus Res 155:175–180

    Article  PubMed  CAS  Google Scholar 

  • Melzer MJ, Sether DM, Karasev AV, Borth W, Hu JS (2008) Complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1. Arch Virol 153:707–714

    Article  PubMed  CAS  Google Scholar 

  • Page RDM (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    PubMed  CAS  Google Scholar 

  • Peng CW, Peremyslov VV, Mushegian AR, Dawson WO, Dolja VV (2001) Functional specialization and evolution of leader proteinases in the family Closteroviridae. J Virol 75:12153–12160

    Article  PubMed  CAS  Google Scholar 

  • Rozanov MN, Koonin EV, Gorbalenya AE (1992) Conservation of the putative methyltransferase domain: a hallmark of the ‘Sindbis-like’ supergroup of positive-strand RNA viruses. J Gen Virol 73:2129–2134

    Article  PubMed  CAS  Google Scholar 

  • Tamada T (2006) Blueberry production in Japan—today and in the future. Acta Hortic 715:267–272

    Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  • Tzanetakis IE, Martin RR (2008) A new method for extraction of double-stranded RNA from plants. J Virol Methods 149:167–170

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported in part by Grant-in-Aid for Scientific Research 21580051 from the Ministry of Education, Science, Sports, and Culture of Japan.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Masamichi Isogai.

Additional information

The nucleotide sequence data reported in this paper are available in the GenBank/EMBL/DDBJ database as accession AB733585 for blueberry virus A.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PPT 206 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Isogai, M., Muramatu, S., Watanabe, M. et al. Complete nucleotide sequence and latency of a novel blueberry-infecting closterovirus. J Gen Plant Pathol 79, 123–127 (2013). https://doi.org/10.1007/s10327-013-0431-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-013-0431-0

Keywords

Navigation