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Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars

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Abstract

Interspecific somatic hybrids between commercial cultivars of potato Solanum tuberosum L. Agave and Delikat and the wild diploid species Solanum cardiophyllum Lindl. (cph) were produced by protoplast electrofusion. The hybrid nature of the regenerated plants was confirmed by flow cytometry, simple sequence repeat (SSR), amplified fragment length polymorphism (AFLP), microsatellite-anchored fragment length polymorphism (MFLP) markers and morphological analysis. Somatic hybrids were assessed for their resistance to Colorado potato beetle (CPB) using a laboratory bioassay, to Potato virus Y (PVY) by mechanical inoculation and field trials, and foliage blight in a greenhouse and by field trials. Twenty-four and 26 somatic hybrids of cph + cv. Agave or cph + cv. Delikat, respectively, showed no symptoms of infection with PVY, of which 3 and 12, respectively, were also resistant to foliage blight. One hybrid of cph + Agave performed best in CPB and PVY resistance tests. Of the somatic hybrids that were evaluated for their morphology and tuber yield in the field for 3 years, four did not differ significantly in tuber yield from the parental and standard cultivars. Progeny of hybrids was obtained by pollinating them with pollen from a cultivar, selfing or cross-pollination. The results confirm that protoplast electrofusion can be used to transfer the CPB, PVY and late blight resistance of cph into somatic hybrids. These resistant somatic hybrids can be used in pre-breeding studies, molecular characterization and for increasing the genetic diversity available for potato breeding by marker-assisted combinatorial introgression into the potato gene pool.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

(∆)(r)AUDPC:

(delta)(relative) Area under disease progress curve

CPB:

Colorado potato beetle

cph :

Solanum cardiophyllum

cv.:

Commercial variety

DAS-ELISA:

Double antibody sandwich enzyme-linked immunosorbent assay

DNA:

Desoxyribonucleic acid

EBA:

Excised-bud-assay

EBN:

Endosperm balance number

GLKS:

The Gross Lüsewitz Potato Collections

IC-RT-PCR:

Immunocapture reverse transcription polymerase chain reaction

IHAR:

Plant breeding and acclimatization Institute

IPK:

Leibniz Institute of Plant Genetics and Crop Plant Research

JKI:

Julius Kühn Institute

MFLP:

Microsatellite-anchored fragment length polymorphism

MRGR:

Mean relative growth rate

Norika:

Nordring-Kartoffelzucht-und Vermehrungs-GmbH Groß Lüsewitz

PVY:

Potato virus Y

RAC:

Station de recherche Agroscope Changins

SASA:

The Scottish agricultural science agency

SH(s):

Somatic hybrid(s)

SSR:

Simple sequence repeat

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Acknowledgments

The German–Romanian and German-Russian Bilateral Projects are acknowledged for supporting part of this research (E. R-T, O. A, T. G). The authors wish to thank Helga Baumann, Hildegard Dreier, Ulrike Busch, Renate Ionasku, Brigitte Deumlich and Petra Hertling for excellent technical assistance. We gratefully thank T. Dixon for his helpful revision of the manuscript.

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Authors and Affiliations

  1. Julius Kühn Institute, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Ramona Thieme, Marion Nachtigall & Thilo Hammann

  2. Babeş-Bolyai University, Clinicilor Str. 5-7, 3400, Cluj-Napoca, Romania

    Elena Rakosy-Tican

  3. Julius Kühn Institute, Federal Research Centre for Cultivated Plants, Institute for Biosafety of Genetically Modified Plants, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Jörg Schubert

  4. Julius Kühn Institute, Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Field Crops and Grassland, Messeweg 11/12, 38104, Braunschweig, Germany

    Udo Heimbach

  5. NI Vavilov Institute of Plant Industry, B. Morskaya Str. 42-44, 190000, St. Petersburg, Russia

    Olga Antonova & Tatjana Gavrilenko

  6. BTL Bio-Test Lab GmbH Sagerheide, Birkenallee 19, 18184, Sagerheide, Germany

    Thomas Thieme

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  1. Ramona Thieme
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  2. Elena Rakosy-Tican
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  3. Marion Nachtigall
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  4. Jörg Schubert
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  7. Tatjana Gavrilenko
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  8. Udo Heimbach
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  9. Thomas Thieme
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Correspondence to Ramona Thieme.

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Communicated by M. Petersen.

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Thieme, R., Rakosy-Tican, E., Nachtigall, M. et al. Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars. Plant Cell Rep 29, 1187–1201 (2010). https://doi.org/10.1007/s00299-010-0905-x

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  • DOI: https://doi.org/10.1007/s00299-010-0905-x

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