Skip to main content

Advertisement

SpringerLink
Log in

Pyrosequencing Analysis of Oral Microbiota in Children with Severe Early Childhood Dental Caries

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Severe early childhood caries are a prevalent public health problem among preschool children throughout the world. However, little is known about the microbiota found in association with severe early childhood caries. Our study aimed to explore the bacterial microbiota of dental plaques to study the etiology of severe early childhood caries through pyrosequencing analysis based on 16S rRNA gene V1–V3 hypervariable regions. Forty participants were enrolled in the study, and we obtained twenty samples of supragingival plaque from caries-free subjects and twenty samples from subjects with severe early childhood caries. A total of 175,918 reads met the quality control standards, and the bacteria found belonged to fourteen phyla and sixty-three genera. Our results show the overall structure and microbial composition of oral bacterial communities, and they suggest that these bacteria may present a core microbiome in the dental plaque microbiota. Three genera, Streptococcus, Granulicatella, and Actinomyces, were increased significantly in children with severe dental cavities. These data may facilitate improvements in the prevention and treatment of severe early childhood caries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, Dewhirst FE, Leys EJ, Paster BJ (2008) Bacteria of dental caries in primary and permanent teeth in children and young adults. J Clin Microbiol 46:1407–1417

    Article  PubMed  CAS  Google Scholar 

  2. Amarante E, Raadal M, Espelid I (1998) Impact of diagnostic criteria on the prevalence of dental caries in Norwegian children aged 5, 12 and 18 years. Community Dent Oral Epidemiol 26:87–94

    Article  PubMed  CAS  Google Scholar 

  3. Arora A, Scott J, Bhole S, Do L, Schwarz E, Blinkhorn A (2011) Early childhood feeding practices and dental caries in preschool children: a multi-centre birth cohort study. BMC Public Health 11:28

    Article  PubMed  Google Scholar 

  4. Caporaso JG, Lauber CL, Costello EK, Berg-Lyons D, Gonzalez A, Stombaugh J, Knights D, Gajer P, Ravel J, Fierer N (2011) Moving pictures of the human microbiome. Genome Biol 12:R50

    Article  PubMed  Google Scholar 

  5. Chao A, Bunge J (2004) Estimating the number of species in a stochastic abundance model. Biometrics 58:531–539

    Article  Google Scholar 

  6. Chao A, Lee S, Jeng S (1992) Estimating population size for capture-recapture data when capture probabilities vary by time and individual animal. Biometrics 48(1):201–216

    Google Scholar 

  7. Cisar J, Kolenbrander P, McIntire F (1979) Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii. Infect Immun 24:742–752

    PubMed  CAS  Google Scholar 

  8. Gross EL, Beall CJ, Kutsch SR, Firestone ND, Leys EJ, Griffen AL (2012) Beyond Streptococcus mutans: dental caries onset linked to multiple species by 16S rRNA community analysis. PLoS One 7:e47722

    Article  PubMed  CAS  Google Scholar 

  9. Hamady M, Walker JJ, Harris JK, Gold NJ, Knight R (2008) Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex. Nat Methods 5:235–237

    Article  PubMed  CAS  Google Scholar 

  10. Hogg SD (1992) Lactic acid bacteria: the lactic acid bacteria in health and disease. 1:115–132

  11. Hong-Ying W, Petersen PE, Jin-You B, Bo-Xue Z (2011) The second national survey of oral health status of children and adults in China. Int Dent J 52:283–290

    Article  Google Scholar 

  12. Huse SM, Ye Y, Zhou Y, Fodor AA (2012) A core human microbiome as viewed through 16S rRNA sequence clusters. PLoS One 7:e34242

    Article  PubMed  CAS  Google Scholar 

  13. Kanasi E, Dewhirst F, Chalmers N, Kent R Jr, Moore A, Hughes C, Pradhan N, Loo C, Tanner A (2010) Clonal analysis of the microbiota of severe early childhood caries. Caries Res 44:485–497

    Article  PubMed  CAS  Google Scholar 

  14. Keijser BJ, Zaura E, Huse SM, van der Vossen JM, Schuren FH, Montijn RC, ten Cate JM, Crielaard W (2008) Pyrosequencing analysis of the oral microflora of healthy adults. J Dent Res 87:1016–1020

    Article  PubMed  CAS  Google Scholar 

  15. Lazarevic V, Whiteson K, Hernandez D, Francois P, Schrenzel J (2010) Study of inter- and intra-individual variations in the salivary microbiota. BMC Genomics 11:523

    Article  PubMed  Google Scholar 

  16. Lazarevic V, Whiteson K, Huse S, Hernandez D, Farinelli L, Osteras M, Schrenzel J, Francois P (2009) Metagenomic study of the oral microbiota by Illumina high-throughput sequencing. J Microbiol Methods 79:266–271

    Article  PubMed  CAS  Google Scholar 

  17. Ling Z, Kong J, Jia P, Wei C, Wang Y, Pan Z, Huang W, Li L, Chen H, Xiang C (2010) Analysis of oral microbiota in children with dental caries by PCR-DGGE and barcoded pyrosequencing. Microb Ecol 60:677–690

    Article  PubMed  CAS  Google Scholar 

  18. Marsh PD (2010) Microbiology of dental plaque biofilms and their role in oral health and caries. Dent Clin North Am 54:441

    Article  PubMed  Google Scholar 

  19. Messer LB (2000) Assessing caries risk in children. Aust Dent J 45:10–16

    Article  PubMed  CAS  Google Scholar 

  20. Peterson SN, Snesrud E, Schork NJ, Bretz WA (2011) Dental caries pathogenicity: a genomic and metagenomic perspective. Int Dent J 61(Suppl 1):11–22

    Article  PubMed  Google Scholar 

  21. Pruesse E, Quast C, Knittel K, Fuchs BM, Ludwig W, Peplies J, Glockner FO (2007) SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res 35:7188–7196

    Article  PubMed  CAS  Google Scholar 

  22. Quevedo B, Giertsen E, Zijnge V, Lüthi-Schaller H, Guggenheim B, Thurnheer T, Gmür R (2011) Phylogenetic group-and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms. BMC Microbiol 11:14

    Article  PubMed  CAS  Google Scholar 

  23. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ (2009) Introducing Mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541

    Article  PubMed  CAS  Google Scholar 

  24. Shen S, Samaranayake LP, Yip HK (2005) Coaggregation profiles of the microflora from root surface caries lesions. Arch Oral Biol 50:23–32

    Article  PubMed  CAS  Google Scholar 

  25. Siqueira JF Jr., Fouad AF, Rocas IN (2012) Pyrosequencing as a tool for better understanding of human microbiomes. J Oral Microbiol 4. doi:10.3402/jom.v4i0.10743

  26. Zaura E, Keijser BJF, Huse SM, Crielaard W (2009) Defining the healthy “core microbiome” of oral microbial communities. BMC Microbiol 9:259. doi:10.1186/1471-2180-9-259

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We acknowledge Dr. Jinhua FANG and colleagues from Affiliated Hospital of Stomatology, Zhejiang University for their clinical assistances. The study was founded by grants 2011 China State key clinical department grants.

Author information

Authors and Affiliations

  1. Department of Conservative Dentistry and Periodontics, Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University, Hangzhou, China

    Wen Jiang, Jie Zhang & Hui Chen

Authors
  1. Wen Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jie Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hui Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hui Chen.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 24 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jiang, W., Zhang, J. & Chen, H. Pyrosequencing Analysis of Oral Microbiota in Children with Severe Early Childhood Dental Caries. Curr Microbiol 67, 537–542 (2013). https://doi.org/10.1007/s00284-013-0393-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-013-0393-7

Keywords

Search

Navigation