Abstract
Pseudomonas stutzeri is a widely distributed species with very high genetic diversity and metabolic capacities, occupying many diverse ecological niches. A collection of 229 P. stutzeri strains isolated from different habitats and geographical locations has been previously characterised phylogenetically by rpoD gene sequencing analysis and in the present study 172 of them phenotypically by whole-cell MALDI-TOF mass spectrometry. Fifty-five strains were further analysed by multilocus sequencing analysis to determine the phylogenetic population structure. Both methods showed coherence in strain grouping; 226 strains were allocated in the 18 genomovars known presently. The remaining three strains are proposed as references for three novel genomovars in the species. The correlation and usefulness of sequence-based phylogenetic analysis and whole-cell MALDI-TOF mass spectrometry, which are essential for autoecological studies in microbial ecology, is discussed for the differentiation of P. stutzeri populations.
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Acknowledgements
C.S. was supported by a grant from the Government of the Balearic Islands (FSE cofunding) and M.G. by a “Juan de la Cierva” contract from the Spanish MINECO. Financial support was obtained from the Spanish MINECO through projects CGL2011-24318 and Consolider CSD2009-00006, as well as funds for competitive research groups from the Government of the Balearic Islands (the last two funds with FEDER cofunding). We thank J. Sikorski and B. Holmes for supplying strains and to J. F. González and R. M. Gomila for their technical assistance in the MALDI-TOF analysis.
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Claudia Scotta and Margarita Gomila contributed equally to this paper
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Scotta, C., Gomila, M., Mulet, M. et al. Whole-Cell MALDI-TOF Mass Spectrometry and Multilocus Sequence Analysis in the Discrimination of Pseudomonas stutzeri Populations: Three Novel Genomovars. Microb Ecol 66, 522–532 (2013). https://doi.org/10.1007/s00248-013-0246-8
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DOI: https://doi.org/10.1007/s00248-013-0246-8