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Fine mapping of a quantitative resistance gene for gray leaf spot of maize (Zea mays L.) derived from teosinte (Z. mays ssp. parviglumis)

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Abstract

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In this study we mapped the QTL Qgls8 for gray leaf spot (GLS) resistance in maize to a ~130 kb region on chromosome 8 including five predicted genes.

Abstract

In previous work, using near isogenic line (NIL) populations in which segments of the teosinte (Zea mays ssp. parviglumis) genome had been introgressed into the background of the maize line B73, we had identified a QTL on chromosome 8, here called Qgls8, for gray leaf spot (GLS) resistance. We identified alternate teosinte alleles at this QTL, one conferring increased GLS resistance and one increased susceptibility relative to the B73 allele. Using segregating populations derived from NIL parents carrying these contrasting alleles, we were able to delimit the QTL region to a ~130 kb (based on the B73 genome) which encompassed five predicted genes.

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Acknowledgements

QY is supported by NSF Grant #1127076, “Genetic and Histological Dissection of Phenotypic Variation in Quantitative Resistance to Maize Diseases”; XY is supported by a China Scholarship Council Fellowship. We are very grateful to Jose Santa-Cruz Hidalgo, Julie Taylor, and Monsanto Inc. for planting and managing our experiments in Andrews NC.

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Authors and Affiliations

  1. Maize Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, China

    Xinye Zhang

  2. Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA

    Xinye Zhang, Qin Yang & Peter Balint-Kurti

  3. Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA, 24061, USA

    Elizabeth Rucker & Wade Thomason

  4. U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS) Plant Science Research Unit, Raleigh, NC, USA

    Peter Balint-Kurti

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  1. Xinye Zhang
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Correspondence to Peter Balint-Kurti.

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Communicated by Mingliang Xu.

Xinye Zhang and Qin Yang have contributed equally to the work.

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Zhang, X., Yang, Q., Rucker, E. et al. Fine mapping of a quantitative resistance gene for gray leaf spot of maize (Zea mays L.) derived from teosinte (Z. mays ssp. parviglumis). Theor Appl Genet 130, 1285–1295 (2017). https://doi.org/10.1007/s00122-017-2888-2

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