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Late blight resistance linkages in a novel cross of the wild potato species Solanum paucissectum (series Piurana)

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Abstract

The cultivated potato, Solanum tuberosum, is affected by a variety of diseases with late blight, caused by Phytophthora infestans, being the most severe. Wild potato species have proven to be a continuing source of resistance, sometimes of an extreme type, to this disease. The present study constructs the first late blight linkage map of a member of series Piurana, S. paucissectum, a tuber-bearing relative of potato, using probes for conserved sequences from potato and tomato. Eight probes mapped to unexpected linkage groups, but syntenic differences with prior maps of potato were not supported by any blocks of rearranged chromosome segments. All 12 linkage groups were resolved and significant associations with late blight resistance were found on chromosomes 10, 11 and 12. A major quantitative trait locus (QTL) on chromosome 11 accounts for more than 25% of the phenotypic variance measured in a field trial. Crossing of S. paucissectum with cultivated potato resulted in very few seeds indicating partial reproductive barriers. Differential reactions of accessions of this potential donor species with simple and complex isolates of P. infestans suggest that it carries major resistance genes that are not those previously described from the Mexican species, S. demissum. However, the additivity of the QTL effects argues for the quantitative nature of resistance in this cross.

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Acknowledgements

We thank Robert Hanneman (deceased) for developing the initial crosses; Leticia Portal, Juan Huaccachi and Julio Patilla for technical assistance; Alberto Salas for species identification and many helpful suggestions; Adelmo Párraga, Universidad Nacional Daniel Alcides Carrión in Oxapampa, Peru, for supervision of field experiments; Ana Sinche and Felipe De Mendiburú of CIP for resistance assessment and statistical consulting; J. Kuhl for the initial screen of accessions for late blight reaction; B. Yandell for statistical consulting; and G. Forbes, M. Havey and J. Jiang for helpful comments on the manuscript. Cluster analysis of disease progress values was proposed by Bodo Trognitz. This work was partially funded by the United States Department of Agriculture-National Research Initiative Grant #00-35300-9411 to David Spooner, Robert Hanneman and M. Bonierbale.

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Authors and Affiliations

  1. Vegetable Crops Research Unit, USDA, Agricultural Research Service, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI, 53706-1590, USA

    F.G. Villamon & D.M. Spooner

  2. Department of Crop Improvement and Genetic Resources, Centro Internacional de la Papa (CIP), 1558, 12, Lima, Peru

    M. Orrillo, E. Mihovilovich, W. Pérez & M. Bonierbale

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  1. F.G. Villamon
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  2. D.M. Spooner
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  3. M. Orrillo
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  4. E. Mihovilovich
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  5. W. Pérez
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  6. M. Bonierbale
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Correspondence to D.M. Spooner.

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Communicated by G. Wenzel

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Villamon, F., Spooner, D., Orrillo, M. et al. Late blight resistance linkages in a novel cross of the wild potato species Solanum paucissectum (series Piurana). Theor Appl Genet 111, 1201–1214 (2005). https://doi.org/10.1007/s00122-005-0053-9

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