Abstract
We report the development of 13 primer pairs that allow the unambiguous amplification of 15 microsatellite (SSR) loci in white spruce (Picea glauca). Fourteen of these loci were polymorphic in trees sampled at three geographically separated regions of western Canada. Segregation analysis carried out on these loci confirmed a Mendelian inheritance pattern for all except two, which showed significant segregation distortion. All of these primer pairs amplified SSR loci in at least one of the other Picea species tested [black spruce (P. mariana), red spruce (P. rubens), Norway spruce (P. abies), Colorado spruce (P. pungens), sitka spruce (P. sitchensis) and Engelmann spruce (P. engelmannii)]. Given the important commercial and ecological roles of these species, this set of markers will be invaluable for their management, the improvement of commercially important traits, and the study of their ecology and genetics.
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Received: 18 August 2000 / Accepted: 28 September 2000
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Hodgetts, R., Aleksiuk, M., Brown, A. et al. Development of microsatellite markers for white spruce (Picea glauca) and related species. Theor Appl Genet 102, 1252–1258 (2001). https://doi.org/10.1007/s00122-001-0546-0
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DOI: https://doi.org/10.1007/s00122-001-0546-0