Abstract
Inflammatory bowel disease (IBD) is a group of severe chronic inflammatory conditions of the human gastrointestinal tract. Murine models of colitis have been invaluable tools to improve the understanding of IBD development and pathogenesis. While the disease etiology of IBD is complex and multifactorial, CD4+ T helper cells have been shown to strongly contribute to the disease pathogenesis of IBD. Here, we present a detailed protocol of the preclinical model of T-cell transfer colitis, which can easily be utilized in the laboratory to study T helper cell functions in intestinal inflammation.
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Acknowledgements
P.M. has been supported by the state of Berlin and the “European Regional Development Fund” (ERDF 2014–2020, EFRE 1.8/11, Deutsches Rheuma-Forschungszentrum) and by EUTRAIN, a FP7 Marie Curie Initial Training Network for Early Stage Researchers funded by the European Union (FP7-PEOPLE-2011-ITN-289903). J.Z. has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 744257. A.A.K. is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—SFB1340 and TRR241.
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Maschmeyer, P., Zimmermann, J., Kühl, A.A. (2021). Murine T-Cell Transfer Colitis as a Model for Inflammatory Bowel Disease. In: Annunziato, F., Maggi, L., Mazzoni, A. (eds) T-Helper Cells. Methods in Molecular Biology, vol 2285. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1311-5_26
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DOI: https://doi.org/10.1007/978-1-0716-1311-5_26
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