Elsevier

Virology

Volume 283, Issue 2, 10 May 2001, Pages 353-357
Virology

Regular Article
Induction of Type-Specific Neutralizing Antibodies by Capsomeres of Human Papillomavirus Type 33

https://doi.org/10.1006/viro.2000.0875Get rights and content
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Abstract

The immunogenicity of capsomeres of human papillomavirus type 33 was evaluated in a dose–response analysis. Capsomeres were obtained free of capsids by expression of L1 carrying the single point mutation C427S. Neutralizing antibodies were detected using an in vitro pseudoinfection assay. Capsomeres induced type-specific, neutralizing antibodies in mice even in the absence of adjuvant. The neutralization titers of immune sera raised without adjuvant were 10- to 20-fold lower than those of antisera to virus-like particles, but virtually identical using Freund's adjuvant. These data indicate that capsomeres may substitute for virus-like particles in future vaccines when used with an adjuvant appropriate for human vaccination.

Keywords

papillomavirus
capsomeres
vaccination
neutralization
dose response

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1

To whom correspondence and reprint requests should be addressed at Institute for Medical Microbiology and Hygiene, Johannes-Gutenberg-Universität Mainz, Hochhaus am Augustusplatz, D-55101 Mainz, Germany. Fax: +49-6131-393-2359. E-mail: [email protected].