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Trypanocidal action of Lippia alba and Lippia origanoides essential oils against Trypanosoma evansi in vitro and in vivo used mice as experimental model

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Abstract

Parasitic diseases have an enormous health and economic impact and are a particular problem in tropical regions of the world. Disease caused by protozoa, such as trypanosomiasis, are the cause of most parasite related morbidity and mortality. Thus, the aim of this study was to verify the trypanocidal effectiveness of Lippia alba and Lippia origanoides against Trypanosoma evansi in vitro and in vivo. L. alba and L. origanoides were used in vitro on trypomastigotes at different concentrations (0.5, 1.0 and 2.0 %) and exposure times (0, 1, 3, 6 and 9 h). The three concentrations tested showed trypanocidal activity in vitro, completely eliminating the parasites in small concentration after 6 h of assay. In vivo tests were performed using mice as the experimental model. T. evansi infected mice were treated with L. alba and L. origanoides with dose of 1.5 mL kg−1 during 5 days. These protocols did not provide curative efficacy, however the mice treated with L. origanoides showed a significant increase in the longevity when compared to control group. Active compounds present in essential oils, such as L. origanoides, may potentiate the treatment of trypanosomosis when associated with other trypanocidal drugs.

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Authors and Affiliations

  1. Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil

    Matheus D. Baldissera

  2. Department of Physiology and Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil

    Carine de Freitas Souza & Silvia G. Monteiro

  3. Graduate Program in Amazonian Natural Resources, Universidade Federal do Oeste do Pará - UFOPA, Santarém, PA, Brazil

    Rosa Helena Veras Mourão & Lenise Vargas Flores da Silva

Authors
  1. Matheus D. Baldissera
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  2. Carine de Freitas Souza
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  3. Rosa Helena Veras Mourão
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  4. Lenise Vargas Flores da Silva
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  5. Silvia G. Monteiro
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Correspondence to Matheus D. Baldissera.

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The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Ethical approval

The procedure was approved by the Animal Welfare Committee of Universidade Federal de Santa Maria under number 117/2014.

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Baldissera, M.D., de Freitas Souza, C., Mourão, R.H.V. et al. Trypanocidal action of Lippia alba and Lippia origanoides essential oils against Trypanosoma evansi in vitro and in vivo used mice as experimental model. J Parasit Dis 41, 345–351 (2017). https://doi.org/10.1007/s12639-016-0800-7

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