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Essential oils with insecticidal activity against larvae of Aedes aegypti (Diptera: Culicidae)

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Abstract

Insecticidal activity of the essential oils (EOs) isolated from Tagetes lucida, Lippia alba, Lippia origanoides, Eucalyptus citriodora, Cymbopogon citratus, Cymbopogon flexuosus, Citrus sinensis, Swinglea glutinosa, and Cananga odorata aromatic plants, grown in Colombia (Bucaramanga, Santander), and of a mixture of L. alba and L. origanoides EOs were evaluated on Aedes (Stegomyia) aegypti Rockefeller larvae. The EOs were extracted by microwave-assisted hydrodistillation and characterized by gas chromatography–mass spectrometry (GC-MS). The main components of the EOs were identified using their linear retention indices and mass spectra. The lethal concentrations (LCs) of the EOs were determined between the third and fourth instar of A. aegypti. LC50 was determined by probit analysis using mortality rates of bioassays. All essential oils tested showed insecticidal activity. The following values were obtained for C. flexuosus (LC50 = 17.1 ppm); C. sinensis (LC50 = 20.6 ppm); the mixture of L. alba and L. origanoides (LC50 = 40.1 ppm); L. alba (LC50 = 42.2 ppm); C. odorata (LC50 = 52.9 ppm); L. origanoides (LC50 = 53.3 ppm); S. glutinosa (LC50 = 65.7 ppm); T. lucida (LC50 = 66.2 ppm); E. citriodora (LC50 = 71.2 ppm); and C. citratus (LC50 = 123.3 ppm). The EO from C. flexuosus, with citral (geranial + neral) as main component, showed the highest larvicidal activity.

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Acknowledgments

This study was conducted thanks to the research support program of the “Vicerrectoría de Investigación of the Universidad Industrial de Santander for Project 5680” and the contributions of the “Patrimonio Autónomo, Fondo Nacional de Financiamiento para la Ciencia, Francisco Jose de Caldas,” contract RC-0572-2012- Bio-Red -CENIVAM. We would also like to thank Dr. German Eduardo Matiz of the University of Cartagena Colombia, for providing the Rockefeller strain.

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Authors and Affiliations

  1. Center for Research on Tropical Diseases (Centro de Investigación en Enfermedades Tropicales-CINTROP), Faculty of Health, School of Medicine, Department of Basic Sciences, Universidad Industrial de Santander, Bucaramanga, Colombia

    Sharon Smith Vera, Diego Fernando Zambrano, Fernando Rodríguez-Sanabria & Jonny E. Duque Luna

  2. Group for Research on Infectious and Metabolic Diseases (Grupo de Investigación en Enfermedades Infecciosas y Metabólicas-GINEM), Faculty of Health, School of Medicine, Department of Basic Sciences, Universidad Industrial de Santander, Bucaramanga, Colombia

    Fernando Rodríguez-Sanabria & Jonny E. Duque Luna

  3. Group for Research in Biochemistry and Microbiology (Grupo de Investigación en Bioquímica Y Microbiología-GIBIM), School of Chemistry, Universidad Industrial de Santander, Bucaramanga, Colombia

    Stelia Carolina Méndez-Sanchez

  4. Center for Research in Biomolecules (Centro de Investigación en Biomoléculas-CIBIMOL) and the National Research Center for the Agro-industrialization of Tropical Medicinal and Aromatic Plants (Centro Nacional de Investigación para la Agroindustrialización de Plantas Aromáticas y Medicinales Tropicales-CENIVAM), Universidad Industrial de Santander, Bucaramanga, Colombia

    Elena E. Stashenko

Authors
  1. Sharon Smith Vera
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  2. Diego Fernando Zambrano
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  3. Stelia Carolina Méndez-Sanchez
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  4. Fernando Rodríguez-Sanabria
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  6. Jonny E. Duque Luna
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Correspondence to Jonny E. Duque Luna.

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Vera, S.S., Zambrano, D.F., Méndez-Sanchez, S.C. et al. Essential oils with insecticidal activity against larvae of Aedes aegypti (Diptera: Culicidae). Parasitol Res 113, 2647–2654 (2014). https://doi.org/10.1007/s00436-014-3917-6

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