Abstract
Chum salmon (Oncorhynchus keta) is an endangered species in China. The numbers of chum salmon have declined sharply in the wild, especially the population entering into the Tumen River (Jilin Province) which is the boundary river between China and North Korea. In order to solve the conflict between China and North Korea about the ownership of the population, we developed ten novel microsatellite loci for future studies of paternity identification.The result showed that the observed and expected heterozygosity ranges were 0.480–0.900 and 0.645–0.885, respectively. The polymorphic information content (PIC) ranged from 0.595 to 0.864, with a mean of 0.776.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Afanas’ev, K.I., Rubtsova, G.A., Malinina, T.V., et al., Microsatellite variability and differentiation of hatchery stocks of chum salmon Oncorhynchus keta Walbaum in Sakhalin, Russ. J. Genet., 2006, vol. 42, pp. 1431–1438.
Beacham, T.D. and Withler, R.E., Population structure of sea-type and lake-type sockeye salmon and kokanee in the Fraser River and Columbia River drainages, PLoS One, 2017, vol. 12, no. 9, art. ID e0183713. https://doi.org/10.1371/journal.pone.0183713
Buchholz, W.G., Miller, S.J., and Spearman, W.J., Isolation and characterization of chum salmon microsatellite loci and use across species, Anim. Genet., 2001, vol. 32, pp. 162–165.
Chen, J.P., Sun, D.J., Dong, C.Z., et al., Genetic analysis of four wild chum salmon Oncorhynchus keta populations in China based on microsatellite markers, Environ. Biol. Fishes, 2005, vol. 73, pp. 181–188.
Guo, S.W. and Thompson, E.A., Performing the exact test of Hardy–Weinberg proportion for multiple alleles, Biometrics, 1992, vol. 48, pp. 361–372.
Kalinowski, S.T., Taper M.L., and Marshall, T.C., Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment, Mol. Ecol., 2007, vol. 16, pp. 1099–1106.
Li, Q. and Wan, J.-M., SSRHunter: development of a local searching software for SSR sites, Yichuan, 2005, vol. 27, pp. 808–810.
Nei, M., Estimation of average heterozygosity and genetic distance from a small number of individuals, Genetics, 1978, vol. 89, pp. 583–590.
Norris, A.T., Bradley, D.G., and Cunningham, E.P., Microsatellite genetic variation between and within farmed and wild Atlantic salmon (Salmo salar) populations, Aquaculture, 1999, vol. 180, pp. 247–264.
Zhan, A., Hu, J., Hu, X., et al., Fine-scale population genetic structure of Zhikong scallop (Chlamys farreri): Do local marine currents drive geographical differentiation?, Mar. Biotechnol., 2009, vol. 11, pp. 223–235.
Funding
This study was supported by the National Infrastructure of Fishery Germplasm Resources (grant no. 2017DKA30470).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interests. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Ye Han, Liu, S., Wan, J. et al. Isolation and Characterization of Ten Novel Microsatellite Loci in Chum Salmon (Oncorhynchus keta). Russ J Mar Biol 45, 491–493 (2019). https://doi.org/10.1134/S1063074019300011
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1063074019300011