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Effects of plant growth regulators on cell growth and alkaloids production by cell cultures of Lycopodiella inundata

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Abstract

Memory deficits associated with neurologically degenerative disorders including Alzheimer’s disease may be partly due to impairment of cholinergic neurotransmission implying that drugs acting as acetylcholinesterase inhibitors are the focus of many therapeutic strategies. Huperziaceae and Lycopodiaceae species have been used in traditional Chinese medicine for the treatment of memory deficiencies. Alkaloids produced by these club mosses species have been shown to produce positive effects on learning and memory. Natural plants are up to now the main source of these compounds and may soon become extinct if harvest practices are not curtailed. Alternative production methods of Lycopodiales alkaloids by plant cell cultures are therefore of growing interest. Previously established in vitro cultures of Lycopodiella inundata have been shown to produce alkaloids but cell growth was very slow. Since plant growth regulators (PGRs) are one of the most important factors affecting cell growth, differentiation and metabolite production, we investigated different cytokinins and auxins, alone or in combination, supplemented in solid media. The aim was to determine their effects on biomass increase and on alkaloids accumulation in plant cell cultures of Lycopodiella inundata. Results analyzed with response surface methodology showed that all PGRs and their interactions strongly affected the plant cell cultures: biomass has been increased by a factor of 5 in 4 months and alkaloid level, closely correlated to PGRs added in culture media, reached 1 % (alkaloid weight/DW) for the optimal conditions. This optimization would be beneficial to large-scale biomass obtention and secondary metabolites production for this species.

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Authors and Affiliations

  1. EDYSAN CNRS FRE 3498, Université de Picardie Jules Verne, 1 rue des Louvels, 80037, Amiens Cedex, France

    Christophe Bienaimé, Aurélie Melin, Lamine Bensaddek, Jacques Attoumbré, Edmundo Nava-Saucedo & Sylvie Baltora-Rosset

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  1. Christophe Bienaimé
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  2. Aurélie Melin
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  3. Lamine Bensaddek
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  4. Jacques Attoumbré
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  5. Edmundo Nava-Saucedo
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  6. Sylvie Baltora-Rosset
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Correspondence to Sylvie Baltora-Rosset.

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Bienaimé, C., Melin, A., Bensaddek, L. et al. Effects of plant growth regulators on cell growth and alkaloids production by cell cultures of Lycopodiella inundata . Plant Cell Tiss Organ Cult 123, 523–533 (2015). https://doi.org/10.1007/s11240-015-0856-6

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  • DOI: https://doi.org/10.1007/s11240-015-0856-6

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