Extraction Procedures for Free Radical Scavenging Activity from Noni Fruit (Morinda citrifolia)

곽미경; 최희선; 홍정화; Benya Manochai

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In this study, we investigated maximum yield of radical scavenging activity (RSA) using each of various extraction solvents and procedure from noni friut (Morinda citrifolia). Noni fruits were either sun-dried (FS) or hot-air dried (FO) at 60℃ after steam blanching. For optimum aqueous extraction, noni fruits should be sun dried and autoclave extraction time should not be over 30 min to produce extraction with high RSA with low cost. In case of 50% ethanol extraction, reflux extracts of FS and FO resulted in IC50 of 1.92 ㎎/mL and 3.06 ㎎/mL at 8 hr. When IC50 values were lower than 5 ㎎/ mL, coefficient of correlation was 0.71 indicating that 71% of the phenolic antioxidants in noni fruits were accounted for the activity by scavenging free DPPH. However, coefficient of correlation significantly decreased to 0.63 over IC50 values of 5 ㎎/ mL. Autoclave extraction contained chlorogenic acid of 14.69 ㎍/mL and scopoletin of 3.86 ㎍/mL. Reflux extraction showed all three compounds, chlorogenic acid (26.19 ㎍/mL), quercetin (19.59 ㎍/mL), and scopoletin (17.4 ㎍/mL). Therefore, the result of this study indicated that the potential antioxidant activities and functional values were obtained significantly with reflux extraction from noni fruit.

#DPPH #Chlorogenic Acid #Noni(Morinda citrifolia) #Quercetin #Scopoletin