1. Developmental Biology

Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling

  1. Xin Huang
  2. Sophie Balmer
  3. Fan Yang
  4. Miguel Fidalgo
  5. Dan Li
  6. Diana Guallar
  7. Anna-Katerina Hadjantonakis  Is a corresponding author
  8. Jianlong Wang  Is a corresponding author
  1. Icahn School of Medicine at Mount Sinai, United States
  2. Memorial Sloan Kettering Cancer Center, United States
  3. College of Veterinary Medicine, Northwest A&F University, China
  4. Centro de Investigacion en Medicina Molecular e Enfermidades Cronicas, Universidade de Santiago de Compostela, Spain
https://doi.org/10.7554/eLife.33333
Share this article
Cite this article
  1. Xin Huang
  2. Sophie Balmer
  3. Fan Yang
  4. Miguel Fidalgo
  5. Dan Li
  6. Diana Guallar
  7. Anna-Katerina Hadjantonakis
  8. Jianlong Wang
(2017)
Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling
eLife 6:e33333.
https://doi.org/10.7554/eLife.33333
  2. Download BibTeX
  3. Download .RIS

Abstract

Pluripotency is defined by a cell's potential to differentiate into any somatic cell type. How pluripotency is transited during embryo implantation, followed by cell lineage specification and establishment of the basic body plan, is poorly understood. Here we report the transcription factor Zfp281 functions in the exit from naive pluripotency occurring coincident with pre-to-post-implantation mouse embryonic development. By characterizing Zfp281 mutant phenotypes and identifying Zfp281 gene targets and protein partners in developing embryos and cultured pluripotent stem cells, we establish critical roles for Zfp281 in activating components of the Nodal signaling pathway and lineage-specific genes. Mechanistically, Zfp281 cooperates with histone acetylation and methylation complexes at target gene enhancers and promoters to exert transcriptional activation and repression, as well as epigenetic control of epiblast maturation leading up to anterior-posterior axis specification. Our study provides a comprehensive molecular model for understanding pluripotent state progressions in vivo during mammalian embryonic development.

https://doi.org/10.7554/eLife.33333.001

Introduction

Development of an organism from a fertilized egg involves the coordination of cell lineage specification coupled with the establishment of cardinal axes (including the anterior-posterior (A-P) axis), to build a blueprint for the body plan (Arnold and Robertson, 2009). The earliest stages of mammalian development culminate in the formation of a blastocyst comprising three cell lineages: the pluripotent epiblast (Epi) which gives rise to somatic and germ cells, and two extra-embryonic lineages, the primitive endoderm (PrE) and trophectoderm (TE) (Schrode et al., 2013). Mouse embryonic stem cells (ESCs) are derived from, and represent an in vitro self-renewing counterpart of, the so-called ‘naive’ pluripotent epiblast cells of the blastocyst (Nichols and Smith, 2009; Boroviak et al., 2014). Pluripotency comprises a continuum of states sequentially encompassing naive, formative and ultimately primed pluripotency (Smith, 2017). Upon blastocyst implantation into the maternal uterus, epiblast cells acquire characteristics of the more developmentally advanced formative and then primed states of pluripotency, which are represented by epiblast-like cells (EpiLCs) (Hayashi et al., 2011) and epiblast stem cells (EpiSCs) (Brons et al., 2007; Tesar et al., 2007; Kojima et al., 2014), respectively. Exploiting the ability of ESCs to transition into EpiLCs/EpiSCs has advanced the mechanistic understanding of epiblast maturation. However, while in vitro studies are instrumental, it remains critical to confirm whether the insights they provide are relevant to events taking place in vivo where the only bona fide pluripotent population, the epiblast, normally exists. The transition to more developmentally advanced states of pluripotency occurs in the mouse embryo coincident with its implantation into the maternal uterus, at around embryonic day (E)4.5–5.0, and is marked by several stereotypical morphological and molecular changes (Bedzhov and Zernicka-Goetz, 2014). Acquisition of the primed state of pluripotency precedes the onset of gastrulation (at E6.25), the process in which the three embryonic germ layers - ectoderm, mesoderm, and endoderm - are formed (Tam et al., 2006; Arnold and Robertson, 2009; Takaoka and Hamada, 2012).

Key events of epiblast maturation include coordinated expression of specific transcription factors (TFs) across developmental stages. Nanog, Klf4, and Rex1 (also named Zfp42) are highly expressed in the epiblast of the blastocyst and ESCs, whereas Fgf5, Oct6 (also named Pou3f1), and Otx2 are upregulated in the epiblast following embryo implantation, or when ESCs differentiate toward EpiSCs. Factors such as Eomes or T (also named Brachyury) are expressed in gastrulating embryos at the primitive streak (the site where pluripotent cells undergo lineage differentiation) and in EpiSCs. Other pluripotency-associated TFs, such as Oct4, Sox2, and Zfp281, are expressed in the pluripotent epiblast throughout these state transitions, suggesting they may play distinct roles in different pluripotent states, or enable transitions between them. Specific DNA modifications and reorganization of enhancer landscapes also occur during the naive-to-primed transition, together with genome-wide relocation of Oct4, as well as elevated binding of Otx2 and the P300 histone acetyltransferase at enhancers of genes specific to the primed state (Buecker et al., 2014; Yang et al., 2014). Concomitantly, during early post-implantation embryo development, the A-P axis is established. A-P patterning is not readily recapitulated in ESC or EpiSC cultures since it necessitates cross-talk between the epiblast and its adjacent extra-embryonic tissue, the visceral endoderm (VE) (Shen, 2007). In the mouse embryo, distal visceral endoderm (DVE) cells, specified at the late blastocyst stage as a sub-population of the PrE, are critical for A-P axis establishment (Beddington and Robertson, 1999; Takaoka and Hamada, 2012). At E5.5, DVE cells are localized at the distal tip of the embryo from where they migrate proximally towards the extra-embryonic/embryonic boundary, recruiting a second population (the anterior visceral endoderm or AVE) and defining an anterior to the embryo, thereby establishing an A-P axis (Stower and Srinivas, 2014). The TGF-beta ligand Nodal, which is expressed by the epiblast, and several of its pathway components, such as the left-right determination factors (Lefty1 and 2) (Brennan et al., 2001), Cripto (also named Tdgf1) (Ding et al., 1998), and Foxh1 (Yamamoto et al., 2001) are required for DVE/AVE specification, migration, and A-P axis formation (Brons et al., 2007; Takaoka and Hamada, 2012). Whether epiblast maturation and A-P axis specification can be mechanistically linked remains an open question.

Zfp281 was recently identified as a TF required for the commitment of ESCs to differentiation in culture (Betschinger et al., 2013; Fidalgo et al., 2016). In this study, we investigate pluripotent state transitions in vivo in their native context, and identify a key role for Zfp281 in early mammalian development. Mouse embryos lacking Zfp281 reach the blastocyst stage and establish a pluripotent epiblast lineage. However, they exhibit defects in epiblast maturation, indicated by the failure to robustly activate Nodal signaling and genes associated with the primed pluripotent state. Hence, they are unable to exit the naive pluripotent state, resulting in a failure to establish an A-P axis. Mechanistically, we demonstrate that Zfp281 functions specifically within the epiblast to coordinate the epigenetic regulators acting to initiate expression of lineage-specific genes and modulate the Nodal signaling pathway.

Results

Zfp281 is expressed in early mouse embryos and required for early post-implantation development

To begin to investigate the role of Zfp281 in vivo during mouse embryonic development when the pluripotent epiblast population is established and matures, we determined the localization of the Zfp281 protein by immunohistochemistry (Figure 1A–C, Figure 1—figure supplement 1A). At E3.5, representing the mid-blastocyst stage, Zfp281 was nuclear-localized and detected at low levels throughout the inner cell mass (ICM) and trophectoderm (TE) (Figure 1B, Figure 1—figure supplement 1A). This widespread expression was maintained until the late blastocyst stage (E4.5) in both ICM derivatives including Epi and PrE, as well as the TE (Figure 1A–B, Figure 1—figure supplement 1A). Single-cell quantitative immunofluorescence (Lou et al., 2014; Saiz et al., 2016) and single-cell microarray data (Ohnishi et al., 2014) revealed that Zfp281 expression was elevated in the epiblast of late stage blastocysts (Figure 1B, Figure 1—figure supplement 1B). At later (post-implantation) stages, Zfp281 expression was restricted to the epiblast and its derivatives (Figure 1A) and absent from the visceral endoderm (VE), a squamous epithelium derived from the PrE, which encapsulates the epiblast (Figure 1C, Figure 1—figure supplement 1C).

Figure 1 with 3 supplements see all
Download asset Open asset
Zfp281 is expressed in the pluripotent epiblast and is required for early post-implantation embryo development.

(A) Single optical sections depicting Zfp281 protein expression in pre- and post-implantation. At pre-implantation, nuclear-localized Zfp281 protein is observed in epiblast (Epi), primitive endoderm (PrE) and trophectoderm (TE) cells, as quantified in (B). Zfp281 expression is specific to epiblast at post-implantation (E5.75), quantified in (B). Immunohistochemistry of Zfp281KO embryo at E5.75 shows that the protein is not expressed, confirming the mutant as a protein null. It also reveals VE-specific background. High-magnification insets (top-right) show protein distribution in regions highlighted, white dashed lines delimit the VE layer. At onset of gastrulation (E6.5), Zfp281 is expressed in all epiblast-derived cells. (B) Quantification of nuclear levels of Zfp281 using MINS software at mid- (E3.5) and late (E4.25–4.5) blastocyst stage, revealing protein expression in all three cell types. n = 5 embryos (308 cells) for mid-blastocyst stage and three embryos (448 cells) for late blastocyst stage. See Figure 1—figure supplement 1 for immunohistochemistry of additional stages. (C) Quantification of nuclear levels of Zfp281 in VE and Epi cells in WT and Zfp281KO embryos at E5.75 using Imaris software. n = 6 embryos for the WT (20 cells per genotype) and n = 3 embryos for Zfp281KO. The expression of Zfp281 in the VE was ruled out through quantitative fluorescence level comparisons of wild-type (WT) and Zfp281KO embryos, which lack Zfp281 protein. (D) At E6.5, the A-P axis is established and WT embryos initiate gastrulation at their posterior, while Zfp281KO embryos display a thickened visceral endoderm epithelium (black arrowhead) and no A-P polarity. Insets (top-right) depict thickened VE layer (delineated by black dashed lines) in Zfp281KO embryo compared to the WT. (E) Zfp281KO embryos die around E8.0 and exhibit aberrant gross morphology at E7.75 when compared to WT with either cells of the epiblast layer undergoing apoptosis and/or constriction at the embryonic/extra-embryonic junction (white arrowheads). A = Anterior, p=Posterior, Pr = proximal, D = Distal, BF = brightfield, Scale bars represent 50 µm. Statistical significance was calculated on the average level of corrected fluorescence per embryo using Student T-test.

https://doi.org/10.7554/eLife.33333.002

Despite its critical function in maintaining ESC pluripotency (Wang et al., 2008; Fidalgo et al., 2011) and promoting the transition from the naive-to-primed state of pluripotency in vitro (Fidalgo et al., 2016), whether Zfp281 plays a role within the epiblast lineage of the developing embryo remained an open question. A conventional knockout (KO) allele of Zfp281 generated using a gene targeting approach (Fidalgo et al., 2011) was used for embryo analysis. No homozygous mutant mice were recovered at birth from intercrosses of heterozygous animals, demonstrating a requirement for Zfp281 in embryonic development (Figure 1—figure supplement 2A). The analysis of staged embryos revealed that mutants died around E8.0 (Figure 1—figure supplement 2B). At E5.5, Zfp281KO embryos were recovered at Mendelian ratios and were indistinguishable from their WT and heterozygous littermates by gross morphology (Figure 1—figure supplement 3A). However, by E6.0–6.5, Zfp281 mutants became readily distinguishable from their WT littermates by their smaller size and distinct morphology, exhibiting a thickened VE layer (Figure 1D, insets, Figure 1—figure supplement 3B, insets with yellow bars). These results suggest a requirement of Zfp281 for both the VE and epiblast, which are likely to be non-cell-autonomous and cell-autonomous, respectively, given the epiblast-specific expression of Zfp281.

To confirm the epiblast-specific function of Zfp281, we produced embryos in which Zfp281 was specifically absent in the epiblast, but in which TE and PrE derivatives were wild-type (WT). To do this we generated tetraploid (4n) WT <->Zfp281KO ESC chimeras and analyzed them at early post-implantation stages (Figure 1—figure supplement 3C). Epiblast-specific loss of Zfp281 produced embryos with a comparable phenotype to that of constitutive gene ablation, with both types of mutant embryos exhibiting a thickened VE (Figure 1—figure supplement 3D). The defect observed in tetraploid chimeras comprising Zfp281KO ESCs could be partially rescued when a Zfp281 cDNA was transfected into Zfp281KO ESCs (referred to as Zfp281KO + Zfp281 cDNA), further confirming the epiblast tissue specificity and Zfp281 gene specificity in the observed mutant phenotypes.

By E7.75, mutant embryos appeared grossly abnormal and displayed either a constriction at the embryonic and extra-embryonic boundary (Figure 1E, white arrowheads), resembling the DVE/AVE defects observed in mutant embryos lacking Eomes (Nowotschin et al., 2013), Otx2 (Ang et al., 1996), Lim1/Lhx1 (Shawlot et al., 1998; Costello et al., 2015), Cripto (Ding et al., 1998), Foxh1 (Yamamoto et al., 2001), and Foxa2 (Ang and Rossant, 1994), or they exhibited extensive cell death and a massive loss of epiblast cells (Figure 1E). Together, these results suggest a cell-autonomous requirement for Zfp281 within the epiblast, and a non-cell-autonomous requirement within the VE.

Deregulation of Nodal signaling and A-P axis specification related genes in Zfp281KO embryos

To identify the molecular changes associated with loss of Zfp281, we characterized the transcriptomic differences between WT and Zfp281KO embryos. We performed RNA sequencing (RNA-seq) on individual E6.5 embryos, corresponding to the earliest stage at which mutants could be morphologically distinguished from WT littermates (Figure 2—figure supplement 1). We identified 968 and 792 transcripts that were significantly downregulated and upregulated, respectively, in Zfp281KO versus WT embryos (Figure 2A, Figure 2—source data 1). Among the significantly downregulated genes were components of the Nodal signaling pathway (e.g., Nodal, Foxh1, Cripto, Lefty1, Lefty2), and genes regionally-restricted in the epiblast and its derivatives (e.g., Fgf5, Otx2, Gsc) or the VE (e.g., Cer1, Lhx1, Dkk1, Hex, Hesx1) (Figure 2A, Figure 2—figure supplement 2). We also identified a number of genes that were significantly upregulated in mutant embryos, including Afp, Patched1 (Ptch1), Gsn (Figure 2A). Gene ontology (GO) analysis for the downregulated genes revealed the top enriched biological process to be A-P axis specification (Figure 2B). Gene set enrichment analysis (GSEA) revealed that Nodal was the top-ranking signaling pathway enriched in the downregulated genes (Figure 2C). Of note, this gene set is also included in the GO term A-P axis specification. We next performed RT-qPCR analysis on E6.5 embryos, and confirmed differential expression of genes that are components of the Nodal signaling pathway, Anterior/AVE markers, and posterior/lineage markers, as well as a few genes that were upregulated in Zfp281KO embryos compared to WT embryos (Figure 2D), consistent with our transcriptome data (Figure 2A). Together, these data demonstrate that Nodal signaling and A-P axis specification, two key events associated with epiblast maturation, were perturbed in the absence of Zfp281.

Figure 2 with 2 supplements see all
Download asset Open asset
Zfp281KO embryos exhibit defects in Nodal signaling and expression of genes associated with anterior-posterior patterning.

(A) Heatmap of genes differentially regulated between WT and Zfp281KO embryos (E6.5). (B) Gene ontology (GO) analysis for significant downregulated genes (fold-change <4, p-value<0.05) in Zfp281KO embryos. (C) Gene set enrichment analysis (GSEA) shows Nodal signaling as top pathway in downregulated genes in Zfp281KO embryos. (D) RT-qPCR for expression of genes at E6.5. For each genotype, n = 6 embryos. mRNA levels of WT embryos were normalized to 1. Student T-test was used examine statistical significance: ns = non significant, *p<0.05, **p<0.01, ***p<0.001. RT-qPCR primer sequences are provided in Figure 2—source data 2.

https://doi.org/10.7554/eLife.33333.006
Figure 2—source data 1

RPKM values of significantly up- and down-regulated genes in WT and Zpf281KO embryos RNA-seq data.

https://doi.org/10.7554/eLife.33333.009
Figure 2—source data 2

RT-qPCR primer sequences.

https://doi.org/10.7554/eLife.33333.010

Zfp281 controls hallmark molecular events in the exit from naive pluripotency

Changes in the expression of some of the stage-specific pluripotency-associated genes (Figure 2) prompted us to investigate this hallmark molecular event involved in the naive-to-primed transition. Under normal development, the pluripotency factors Sox2 and Oct4 are similarly expressed throughout the naive-to-primed transition. On the other hand, Nanog is rapidly shut down after E4.5 (Chambers et al., 2003) and expressed again from E6.0 onwards in the epiblast (Hart et al., 2004). Nanog is also downregulated in EpiSCs (Silva et al., 2009). Consistent with our RNA-seq data, we noted that Sox2 and Nanog protein levels were unaffected in Zfp281KO embryos (Figure 3A,D and Figure 3—figure supplement 1) or in tetraploid chimeras (Figure 3—figure supplement 2A). In contrast, Oct4 protein was significantly downregulated in E6.25 Zfp281KO embryos (Figure 3B,D). Additionally, Otx2, which is activated during the naive-to-primed transition and critical for activation of epiblast gene-related enhancers (Buecker et al., 2014), was expressed at significantly reduced levels (Figure 3C,D) and mislocalized at the distal tip of mutant embryos, rather than being anteriorly restricted as in WT (Figure 3—figure supplement 2B). This downregulation of Otx2 protein expression was already visible at an earlier stage in Zfp281KO embryos and in tetraploid chimeras, together with the downregulation of another marker of primed pluripotency, Oct6 (Figure 3—figure supplement 2C), further indicating that epiblast maturation is defective in Zfp281KO embryos.

Figure 3 with 3 supplements see all
Download asset Open asset
Zfp281 regulates pluripotency-associated factors and Otx2.

(A) Immunostaining of Nanog and Sox2 in WT and Zfp281KO embryos (E6.25). (B) Immunostaining of Oct4 in WT and Zfp281KO embryos (E6.25). (C) Immunostaining of Otx2 in WT and Zfp281KO embryos (E6.75). (D) Fluorescent intensity quantification of Nanog, Sox2, Oct4 and Otx2 proteins (each dot representing the mean corrected fluorescence level per epiblast cell) using Imaris software. For each genotype, n = 3 embryos (30 cells quantified per embryo). Statistical significance was calculated on the average level of corrected fluorescence per embryo using Student T-test. (E) Immunohistochemistry of pluripotency factor Nanog which is localized to posterior epiblast in WT embryos, and the primitive streak marker T, exhibit radialized expression in mutant compared to WT (E7.0). Additionally, in Zfp281KO, the mutually-exclusive pattern of Nanog and T observed in WT is lost and proteins colocalize in a subset of cells. High magnification insets (top-left) show distribution in regions highlighted. (F) WISH of E6.75 Zfp281KO and WT littermate embryos. Markers of the primitive streak T and Fgf8 are radialized in Zfp281KO embryos compared to WT. A = Anterior, p=Posterior, Pr = proximal, D = Distal, Scale bars represent 50 µm.

https://doi.org/10.7554/eLife.33333.011

Another hallmark of the naive-to-primed transition is the expression of lineage-specific genes at the posterior part of the embryo, marking the site of the primitive streak and onset of gastrulation. We noted that the T protein is expressed in Zfp281KO embryos (Figure 3E). However, cells that started expressing T, had not downregulated Nanog (see inset in Figure 3E), indicating a failure in the extinguishment of pluripotency-associated genes as epiblast cells committed to differentiate in Zfp281KO embryos. The domains of both T and Nanog expression in mutant embryos were also proximally radialized, instead of being posteriorly restricted as in WT embryos (Figure 3E). This radialization of normally posteriorly-localized markers was also observed in tetraploid Zfp281KO ESCs chimeras (Figure 3—figure supplement 3A). Notably, this phenotype was rescued in tetraploid WT <->Zfp281KO + Zfp281 cDNA ESC chimeras (Figure 3—figure supplement 3A). Analysis of RNA expression by wholemount mRNA in situ hybridization (WISH) of other markers of the primitive streak such as Fgf8, Axin2 and Eomes showed that, similarly to T, they were expressed and proximally radialized in mutant embryos (Figure 3F and Figure 3—figure supplement 3B,C). Our WISH and RNA-seq data both also revealed that levels of Fgf8 were reduced upon Zfp281 loss. However, T, Axin2 and Eomes RNA levels were not significantly reduced in Zfp281KO embryos (Figure 2D). Therefore, Zfp281 is required for induction and proper localization of lineage specification markers.

Together, our data establish a requirement for Zfp281 in controlling key molecular events involved in the naive-to-primed transition in embryos, including extinguishment of pluripotency-associated and induction (or localization) of lineage specification transcriptional programs during epiblast maturation.

Zfp281 plays a critical role in Nodal signaling activation to promote DVE/AVE migration

Execution of the naive-to-primed transition in the epiblast of early post-implantation embryos culminates in A-P axis formation, determined via cross-talk between the epiblast and VE through Nodal and Wnt signals (Kiecker et al., 2016). The radialized expression of Nanog and several lineage specification factors in Zfp281KO embryos (Figure 3E–F, Figure 3—figure supplement 2B and Figure 3—figure supplement 3A–B), as well as deregulation of genes involved in A-P axis specification (Figure 2) prompted us to examine the expression and localization of AVE/DVE markers. WISH of Hesx1, Dkk1, Cer1, Hex, and Lefty1 revealed their absence or significantly reduced expression in Zfp281KO embryos (Figure 4A and Figure 4—figure supplement 1). WISH for Dkk1 of Zfp281KO tetraploid chimera confirmed this phenotype and showed a partial rescue using Zfp281KO + Zfp281 cDNA ESCs (Figure 4—figure supplement 2A). In the few cases where expression was detected (e.g., Hesx1 in Zfp281KO or Dkk1 in tetraploid mutant chimera), it was localized at the distal tip of embryos, but not anteriorly as in WT (Figure 4A, Figure 4—figure supplement 1 and Figure 4—figure supplement 2A), suggesting that the DVE/AVE population was not specified, or could not be maintained or migrate. To distinguish these possibilities, we employed a Hex-GFP reporter line (Rodriguez et al., 2001) to visualize the AVE in Zfp281KO embryos. In agreement with our WISH data, Hex-GFP expression was reduced and distally-localized, consistent with a failure in maintenance leading to impaired migration of the DVE/AVE population (Figure 4B and Figure 4—figure supplement 2B). By contrast, the expression of proteins labeling the entire VE, such as Gata6, Lhx1 and Eomes, was unaffected in Zfp281 mutants (Figure 4—figure supplement 2C,D).

Figure 4 with 2 supplements see all
Download asset Open asset
Loss of Zfp281 affects pluripotent state progression and results in failure in anterior-posterior axis establishment.

(A) WISH of E6.5 Zfp281KO and WT littermate embryos. Markers of DVE/AVE such as Hesx1, Dkk1, Cer1, Hex and Lefty1 are not expressed in Zfp281KO embryos compared to WT littermates, which exhibit distal-anterior domains of expression. Red arrowheads indicate the position of most anterior cells of the DVE/AVE. (B) Reduced expression and failure of anterior migration of Hex-GFP reporter in Zfp281KO embryos. Cell shape and organization within the VE layer is affected by absence of Zfp281 as indicated by distribution of F-Actin. White dashed line indicates the limit between extra-embryonic and embryonic regions. Quantification of nuclear levels of Hex-GFP at E5.75 (n = 2 embryos, 89 cells for WT and n = 3 embryos, 78 cells for Zfp281KO) and at E6.5 (n = 2 embryos, 193 cells for WT and n = 2 embryos, 89 cells for Zfp281KO) using Imaris software. Statistical significance was calculated on the average level of corrected fluorescence per embryo using Student’s T-test. *p<0.05. (C) WISH analysis of the Nodal target Lefty2 shows loss of expression in Zfp281KO embryos. Nodal is radialized in Zfp281KO embryos compared to WT due to lack of A-P axis. A = Anterior, p=Posterior, Pr = Proximal, D = Distal, 2D = single optical section, 3D = projection of several optical section. Scale bars represent 50 µm.

https://doi.org/10.7554/eLife.33333.015

Nodal and Wnt signaling pathways play a key role in DVE/AVE specification and migration (Kiecker et al., 2016), and rank as top enriched GO terms in the downregulated genes from our RNA-seq data (Figure 2C). We already showed that levels of Axin2 and T, two Wnt signaling targets, were unaffected by loss of Zfp281, although their expression was radialized due to failure in A-P axis specification (Figures 2D and 3F and Figure 3—figure supplement 3B). By contrast, the Nodal target and negative regulator Lefty2 (expressed in the posterior epiblast of WT embryos) were absent in the epiblast of Zfp281KO embryos (Figure 4C), similarly to the related Lefty1 gene, which is expressed earlier in the VE, and also absent in mutants (Figure 4A). Nodal and Eomes are usually posteriorly localized in the epiblast at the site of the primitive streak (Nowotschin et al., 2013). However, their expression was radialized in Zfp281KO embryos (Figure 4C and Figure 3—figure supplement 3B) while Nodal expression levels were reduced (Figure 2D and Figure 4—figure supplement 2E).

Together, our data suggest an impairment of Nodal signaling in Zfp281KO embryos, leading to defects in DVE/AVE specification and migration, further corroborating a critical role for Zfp281 in promoting epiblast maturation.

Zfp281 regulates lineage-specific genes for transcriptional activation during epiblast maturation

To understand the molecular mechanisms by which Zfp281 promotes epiblast maturation, we turned to the in vitro naive-to-primed transition model to identify Zfp281-regulated genes. We first examined expression of Zfp281-regulated genes in WT and Zfp281KO ESCs and epiblast-like cells (EpiLCs), which is an alternative of primed cells because Zfp281KO is detrimental to the self-renewal of EpiSCs (Tsakiridis et al., 2014; Fidalgo et al., 2016). EpiLCs are derived by short-time (48 hr) adaption of ESCs to the primed cell culture condition, representing an intermediate state, known as the formative state (Kalkan and Smith, 2014; Smith, 2017), in the naive-to-primed transition (Hayashi et al., 2011; Buecker et al., 2014). As expected, EpiLCs (WT) expressed higher levels of lineage-specific genes than ESCs (Figure 5A). However, levels of expression of these genes were reduced or abrogated in Zfp281KO relative to WT EpiLCs (Figure 5A), indicating Zfp281 is important for establishing or maintaining expression of these genes during the transition. In addition, while genes involved in Nodal signaling were similarly expressed in both WT ESCs and EpiLCs, their levels of expression were downregulated in Zfp281KO versus WT EpiLCs (Figure 5—figure supplement 1), consistent with their downregulation in Zfp281KO versus WT embryos (Figure 2A,D).

Figure 5 with 2 supplements see all
Download asset Open asset
Zfp281 activates lineage-specific genes during epiblast maturation.

(A) Expressions of lineage-specific genes in WT and Zfp281KO ESCs and EpiLCs, from a previous published RNA-seq dataset (Fidalgo et al., 2016). mRNA expression level is mapped reads per million total mapped reads per kilobase (RPKM). (B) Zfp281 and H3K27me3 ChIP-seq tracks at loci of T, Otx2, Eomes, and Gsc. Black bar under track indicates region called as Zfp281 peak by MACS software. (C) ChIP-qPCR of Zfp281 at promoters (Pro) of T, Otx2, Eomes, and Gsc in ESCs and EpiSCs. Promoter and negative regions for PCR at each gene are shown in panel B. (D) ChIP-qPCR of H3K27me3 at promoters of T, Otx2, Eomes, and Gsc. mCh6 is a negative control mapping to a gene desert region on chromosome 6 (Boyer et al., 2006). (E) Zfp281 and H3K27ac ChIP-seq tracks at loci of Fgf5 and Fgf8. Black bar under track indicates region called as Zfp281 peak by MACS software. (F) ChIP-qPCR of Zfp281 at regulatory regions of Fgf5 and Fgf8 in ESCs and EpiSCs. Positions for PCR at each gene are shown in panel E. (G) ChIP-qPCR of H3K27me3 at regulatory regions of Fgf5 and Fgf8. mCh6 is a negative control mapping to a gene desert region on chromosome 6 (Boyer et al., 2006). ChIP-qPCR primer sequences are provided in Figure 5—source data 1.

https://doi.org/10.7554/eLife.33333.018
Figure 5—source data 1

ChIP-qPCR primer sequences.

https://doi.org/10.7554/eLife.33333.021

To investigate how Zfp281 exerts transcriptional control of downstream target genes, we performed chromatin immunoprecipitation (ChIP) with massively parallel sequencing (ChIP-seq) in both WT ESCs and EpiSCs, which revealed enrichment of Zfp281 binding at regions near gene transcription start sites (TSSs) and enhancers (Figure 5—figure supplement 2A,B), suggesting that Zfp281 is actively involved in transcriptional regulation in both naive and primed pluripotent states. There were 9358 common peaks for Zfp281, and 11,408 and 3467 peaks specific to ESCs and EpiSCs, respectively, which were lost and gained during the transition between these two states (Figure 5—figure supplement 2C,D), suggesting Zfp281 targets may be dynamically regulated during pluripotent state transition.

Next, we determined whether Zfp281 coordinately controls transcriptional programs associated with pluripotent state transition and lineage commitment through binding of regulatory regions of its target genes. Surprisingly, promoters (Pro) of lineage-specific genes such as T, Otx2, Eomes, Gsc were bound by Zfp281 with a higher enrichment in ESCs than in EpiSCs (Figure 5B). ChIP-qPCR analyses confirmed that Zfp281 binding intensities were reduced at these promoters in EpiSCs (Figure 5C). Consistent with the fact that these lineage-specific genes will be activated in primed cells, we also observed diminished intensities of repressive histone mark H3K27me3 on their promoters (Figure 5D). By contrast, there was no Zfp281 peak at promoters of Fgf5 or Fgf8, indicating a promoter-independent regulation of Zfp281 on these two genes (Figure 5E). However, Zfp281 bound at promoter-distal regions of Fgf5 and Fgf8 (Figure 5E–F) accompanied with increased H3K27ac (Figure 5G) in EpiSCs, suggesting Zfp281 may be involved in enhancer activation on these two targets during the naive-to-primed transition. Indeed, a previous study has shown that the two Zfp281 peaks (P1, P2) comprising enhancers of Fgf5 are critical for the naive-to-primed transition (Buecker et al., 2014). Taken together, our data indicate that Zfp281 regulates lineage-specific genes during epiblast maturation through both promoter- and enhancer-related mechanisms.

Zfp281 is associated with chromatin modifiers for promoter activation of lineage-specific genes

To further understand how Zfp281 controls transcription of target genes in relation to their promoter chromatin architecture, we investigated the genome-wide association of Zfp281 with other epigenetic regulators and TFs in ESCs. Hierarchical clustering analysis for ChIP-seq association revealed that Zfp281 and Ep400 have the most similar binding patterns. Furthermore, Zfp281 and Ep400 also show similar binding patterns with the Polycomb repressive complex 2 (PRC2) components Ezh2 and Suz12 (Figure 6A). Ep400 is a component of the Tip60-Ep400 histone acetyltransferase complex that is necessary to maintain ESC self-renewal (Fazzio et al., 2008; Chen et al., 2015). A previous study showed that Ep400 localization to promoters depends on H3K4me3, and Ep400 promotes histone H4 acetylation at both active and silent target promoters in ESCs (Fazzio et al., 2008). We profiled ChIP-seq intensities of Ep400, Suz12 (a component of PRC2 that modifies H3K27me3), Mbd3 and histone marks H3K4me3, H3K27ac, and H3K27me3 at Zfp281 peak regions (Figure 6B). Mbd3 is a core component of NuRD histone deacetylation complex that can be recruited by Zfp281 for repression of pluripotency genes (Fidalgo et al., 2012). Zfp281 and Ep400 peaks exhibited a high correlation across the genome, but were mutually exclusive with Suz12 and Mbd3 peaks, dividing Zfp281 peaks into two classes: (I) Zfp281/Ep400/Suz12-cobound, and (II) Zfp281/Ep400/Mbd3-cobound (Figure 6B). Class I and Class II characterize the epigenetic features of target genes in ESCs, and are highly enriched for GO terms that signify development and pluripotency, respectively (Figure 6—figure supplement 1). The Class I genes are bivalent with a feature of H3K4me3 and H3K27me3 co-enrichment (Figure 6B). Bivalent genes remain silent in ESCs while undergoing fast activation in response to differentiation signals (Bernstein et al., 2006). Our study demonstrates that Zfp281 regulates active and bivalent genes by associating with identical (Ep400 for both Class I and Class II) or distinct (PRC2 for Class I versus NuRD for Class II) epigenetic regulators.

Figure 6 with 1 supplement see all
Download asset Open asset
Zfp281 associates with PRC2 and Ep400 complexes for promoter regulation of bivalent lineage genes.

(A) Heatmap of ChIP-seq association analysis of Zfp281 with other transcription factors and epigenetic regulators in ESCs. The ChIP-seq datasets were compiled from published studies (see Figure 6—source data 1 for the accessions). The scale represents Phi correlation coefficient. (B) ChIP-seq profiles of Zfp281, Ep400, Suz12, Mbd3, and histone marks H3K4me3, H3K27me3, and H3K27ac at Zfp281 peak regions. Intensity is plotted within 5 kb around Zfp281 peak center. Two classes of Zfp281 peaks are shown: Class I, Zfp281-Mll2-Ep400-Suz12 co-bound; Class II, Zfp281-Mll2-Ep400-Mbd3 co-bound. (C) Co-immunoprecipitation (co-IP) of Zfp281 with other epigenetic regulators in ESCs and EpiSCs. (D) ChIP-seq tracks of Zfp281 and PRC2 complex (Suz12) at bivalent T and Eomes promoters. (E) ChIP-qPCR shows Suz12 binding at T and Eomes promoters decreases in Zfp281KO ESCs.

https://doi.org/10.7554/eLife.33333.022
Figure 6—source data 1

Accession numbers of ChIP-seq data used in Figure 6A.

https://doi.org/10.7554/eLife.33333.024

We next asked whether Zfp281 associates with these epigenetic modifiers during the naive-to-primed transition. While we confirmed interactions of the PRC2 (Suz12) and NuRD (Chd4, Mbd3) complexes with Zfp281 in ESCs, we found their associations with Zfp281 were reduced in EpiSCs despite their expression levels being similar in the two populations (Figure 6C). By contrast, Zfp281's interaction with components of the Tip60-Ep400 complex (Ep400, Trrap) was maintained in both ESCs and EpiSCs (Figure 6C). Loss of interaction between Zfp281 and the PRC2 complex may be responsible for the activation of Zfp281 target genes during the transition, providing a parsimonious explanation for the upregulation of lineage specification genes (Figure 5B–C) and reduction of H3K27me3 (Figure 5D) in EpiSCs versus ESCs. The lack of association between Zfp281 and NuRD in EpiSCs may also explain why pluripotency genes including Sox2 and Nanog are not regulated by Zfp281 in the epiblast (Figure 3A,D), which is in contrast with NuRD-mediated Nanog repression in ESCs due to their physical association (Fidalgo et al., 2012). We confirmed the reduced chromatin occupancy of Suz12 at bivalent promoters of T and Eomes in Zfp281KO relative to WT ESCs (Figure 6D–E), indicating a Zfp281-dependent recruitment of PRC2 at the bivalent promoters. However, we cannot exclude the possibility that the residual binding of Zfp281 to bivalent promoters (e.g., T and Eomes in Figure 5C) with maintained Tip60-Ep400 complex association during the naive-to-primed transition may further reinforce the activation of lineage-specific genes through the presumed role of Tip60-Ep400 in outcompeting PRC2, and thus downregulating the promoter H3K27me3 levels (Chen et al., 2015).

Taken together, our data reveal an important role of Zfp281 in regulating bivalent promoters during the naive-to-primed pluripotency transition. Reduced association between Zfp281 and the PRC2 complex, but preservation of the Zfp281-Ep400 association, at bivalent promoters results in decreased H3K27me3 during this transition, leading to transcriptional activation of lineage-specific genes concomitant with epiblast maturation.

Zfp281 cooperates with Oct4 and P300 for regulation of Nodal signaling components in epiblast maturation

We have shown that Zfp281 is necessary for activation of Nodal signaling components in the embryo (Figure 2 and Figure 4). Next, we investigated activity of Nodal signaling by examining Smad2 phosphorylation (p-Smad2) in WT and Zfp281KO ESCs. P-Smad2 is significantly reduced in Zfp281KO ESCs compared to that in WT ESCs (Figure 7A). Since Zfp281KO EpiSCs cannot be maintained in long-term culture (Fidalgo et al., 2016), we performed shRNA-mediated knockdown (KD) of Zfp281 in EpiSCs. P-Smad2 is not affected by Zfp281KD in EpiSCs, probably because of the Activin in culture constitutively activating p-Smad2. However, protein expression of the Nodal signaling target Lefty significantly decreased by Zfp281KD (Figure 7—figure supplement 1A), suggesting that Zfp281 may directly regulate Lefty expression. Similarly, reduction of Lefty protein expression is reproduced by treatment of ALK receptor inhibitor (ALKi) that specifically blocks p-Smad2 and Nodal signaling (Figure 7—figure supplement 1B). In addition, as activation of WNT and Nodal signaling pathways can differentiate ESCs to primitive-streak (PS)-like cells (Mulas et al., 2017), we also investigated the role of Zfp281 in this differentiation. ESCs were treated with Activin (a Nodal ligand) and CHIR (a GSK3β inhibitor to activate WNT pathway), and with ALKi or Zfp281 shRNAs. The morphology of Zfp281KD cells revealed a strong phenotype of differentiation resistance, which is similar to that of ALKi treatment. Dome-shaped ESC-like colonies persisted in Zfp281KD cell cultures after Activin/CHIR treatment, a striking difference compared to WT control cells (Figure 7—figure supplement 2A). RT-qPCR for up to 3 days after treatment indicated both Zfp281KD- and ALKi-treated cells exhibited decreased expression of the PS marker genes T and Lefty2 (a Nodal signaling target gene also expressed in PS) (Figure 7—figure supplement 2B,C).

Figure 7 with 3 supplements see all
Download asset Open asset
Zfp281 cooperates with Oct4 and P300 for regulation of genes encoding Nodal pathway components.

(A) Protein expression of p-Smad2 and Smad2 in WT (J1, CJ7, WT clone no 3), Zfp281KO (clone no. 2.6, 7, 3.34) ESC and WT EpiSC (OEC2, EpiSC9) cells. P-Smad2/Smad2 density ratios were quantified on the right panel. Student T-test was used examine statistical significance, *p<0.05. (B) Co-IP of Zfp281 with Oct4 and P300 in EpiSCs. The non-specific band is IgG heavy chain. (C) ChIP-seq tracks of Zfp281, Oct4 and P300 in ESCs and EpiSCs/EpiLCs at Nodal, Lefty2, and Foxh1 loci. Intensities are shown as mapped reads per million total mapped (RPM). (D) ChIP-qPCR of Zfp281 at Nodal, Lefty2, and Foxh1 regulatory regions in in ESCs and EpiSCs. (E) ChIP-qPCR of histone markers H3K27me at Nodal, Lefty2, and Foxh1 regulatory regions in ESCs and EpiSCs. (F) Diagram of ChIP experiments on E6.5 (WT) embryos. (G) ChIP-qPCR of Zfp281 at Nodal, Lefty2, and Foxh1 regulatory regions in E6.5 (WT) embryos. ChIP experiments were performed in two independent replicates. ChIP-qPCR primer sequences are provided in Figure 7—source data 1.

https://doi.org/10.7554/eLife.33333.025
Figure 7—source data 1

ChIP-qPCR primer sequences.

https://doi.org/10.7554/eLife.33333.029

To understand the molecular regulation of the Nodal signaling pathway by Zfp281, we employed ChIP-seq analysis and revealed that Zfp281 localizes to distal regions of Nodal and Lefty2, as well as promoters of Foxh1 (Figure 7C). Previous studies indicated that transcription factors Oct4, Otx2, and histone acetyltransferase P300 (the writer of H3K27ac) associate with enhancer reorganization in the naive-to-primed transition (Buecker et al., 2014; Yang et al., 2014). We first confirmed that Zfp281 interacts with Oct4 and P300 in EpiSCs (Figure 7B). However, we did not detect the Zfp281-Otx2 interaction in EpiSCs (data not shown). Furthermore, ChIP-seq analysis indicates that Zfp281 co-localizes with Oct4 and P300 (Buecker et al., 2014) in almost all Zfp281 peaks (Figure 7C, Figure 7—figure supplement 3), suggesting that Zfp281 may be involved in widespread relocation of Oct4 and P300, including both promoters and enhancers, in the naive-to-primed transition, that is independent of Otx2 association.

Zfp281 localizes to characterized enhancers within the Nodal locus (PEE, NDE, HBE, but not ASE, nomenclature from [Papanayotou et al., 2014]) (Figure 7C). ChIP-qPCR analysis confirmed that Zfp281 intensities are comparable between PEE and NDE, but decreased at HBE in EpiSCs compared to ESCs (Figure 7D). Zfp281 intensity by ChIP-qPCR was relatively low at ASE, consistent with our ChIP-seq data (Figure 7C–D). It is reported that, during the naive-to-primed transition, Nodal enhancer activity relocates from the HBE to the ASE (Papanayotou et al., 2014). Indeed, H3K27ac intensity was also reduced at HBE (Figure 7E), consistent with reduced Zfp281 binding at this locus during the naive-to-primed transition (Figure 7D). We also evaluated Zfp281 intensities by ChIP-qPCR at an enhancer of Lefty2 and the promoter of Foxh1, and found high intensities of Zfp281 and H3K27ac, colocalizing with P300 and Oct4 peaks at these loci in ESCs and EpiSCs (Figure 7C–E). Zfp281 binding at regulatory regions of these Nodal signaling-related genes is biologically important, as expression of Lefty2 is downregulated by Zfp281KD in EpiSCs (Figure 7—figure supplement 1), and many of Nodal signaling genes were perturbed in Zfp281KO EpiLCs relative to their WT counterparts (Figure 5—figure supplement 1), supporting a critical role of Zfp281 in transcriptional activation of Nodal signaling during the transition to primed pluripotency in vitro. To provide direct evidence that Zfp281 binds and regulates these Nodal signaling related genes in vivo, we performed ChIP experiments on E6.5 WT embryos (Figure 7F). Zfp281 exhibited high chromatin-binding activity at the HBE enhancer of the Nodal locus, as well as the regulatory regions within the Lefty2 and Foxh1 loci (Figure 7G), which would be abrogated in Zfp281 mutant embryos. Together, our data demonstrate that Zfp281, together with P300 and Oct4, are important for regulation of components of the Nodal signaling pathway during the maturation of the epiblast of the mouse embryo.

Discussion

Pluripotency is a continuum where the naive and primed states represent the initial and final stages, corresponding to the establishment of pluripotency in the pre-implantation blastocyst and the exit from pluripotency as cells of the post-implantation epiblast initiate gastrulation. While the in vitro pluripotent state transition model provides a useful tool to infer how the epiblast transitions between states and prepares for germ layer differentiation (Weinberger et al., 2016), it cannot substitute for direct investigations into the transition occurring during epiblast maturation in vivo in embryos. Notably, none of the pluripotency-associated factors, for which mouse mutants are available, have described phenotypes directly associated with the exit from naive pluripotency in the embryo. Zfp281 is the first factor demonstrated to be critical in vivo for this developmental transition; its loss results in embryonic lethality due to a failure in epiblast maturation. Our data reveal that Zfp281 is concomitantly required for the initiation of expression of genes encoding Nodal signaling components and the lineage specification program during epiblast maturation. Zfp281 coordinates crosstalk among multiple epigenetic pathways through physical association with histone methylation (PRC2) and acetylation (Ep400, P300) complexes within epiblast cells. Crosstalk converges on the coordinated regulation of bivalent promoters and reorganization of enhancers leading to activation of lineage-specific genes and downregulation of pluripotency genes during the naive-to-primed transition, arguing for a master regulator status of Zfp281 in controlling key molecular events leading to epiblast maturation (Figure 8, top panel). Accordingly, loss of Zfp281 results in a series of developmental defects within the epiblast, which non-cell-autonomously lead to a failure to establish or maintain the DVE/AVE resulting in A-P axis specification defects (Figure 8, bottom panel).

Figure 8
Download asset Open asset
Working model for the role of Zfp281 in epiblast maturation.

Upper panel describes a working model for Zfp281 function during epiblast maturation in the post-implantation embryo. Zfp281 is expressed in epiblast cells (blue) and directs the activation of target genes. The lower panel describes the Zfp281KO embryo phenotype. Defects in epiblast maturation and Nodal signaling lead to failure in anterior-posterior axis establishment. Markers of the posterior epiblast (red) are radialized.

https://doi.org/10.7554/eLife.33333.030

The in vitro pluripotent stem cell models overcome the limitation of embryo material and so have been instrumental in dissecting molecular events involved in the naive-to-primed transition (Buecker et al., 2014; Factor et al., 2014) as well as the regulation of the Nodal signaling pathway during epiblast maturation (Papanayotou et al., 2014; Papanayotou and Collignon, 2014). Our combined in vitro and in vivo studies have demonstrated high consistency of the regulatory functions of Zfp281 on the lineage-specific genes (Figure 2 and Figure 5). However, there are also some differences between our findings in embryos and those made in pluripotent stem cell models. For instance, embryo RNA-seq data indicated non-significant changes in expression of lineage markers T and Eomes between WT and Zfp281KO embryos (Figure 2D), while their expression was markedly downregulated in Zfp281KO EpiLCs (Figure 5A). This disparity could be due to EpiLC representing a pluripotent state that does not match with the embryonic stage examined for RNA-seq in embryos, and/or extra-embryonic expression, in the case of Eomes in vivo (Nowotschin et al., 2013) (Figure 3—figure supplement 3B), that is not represented in EpiLC culture. Similarly, Zfp281KO embryonic phenotypes described for the VE layer cannot be reproduced in vitro since there is no equivalent cell population in ESC/EpiSC cultures. We noted that the morphology of VE cells in Zfp281KO embryos was different from the WT and resembled their adjacent extra-embryonic VE cells (Figure 1—figure supplement 3). As we identified that Afp, a pan-VE marker whose downregulation is coincident with the onset of gastrulation (Viotti et al., 2014), is upregulated in our mutant embryos (Figure 2A,D), we speculate that Zfp281KO embryos may also fail in VE maturation.

Furthermore, we also observed that Nodal signaling was significantly reduced in Zfp281KO embryos (Figure 2D), whereas in EpiSCs, p-Smad2 level was not affected by Zfp281KD (Figure 7—figure supplement 1). We speculate that this discrepancy may be attributable to the presence of Activin in the primed cell culture medium, which constitutively activates Nodal signaling. Compared to the in vitro system, crosstalk between the in vivo epiblast and its adjacent extra-embryonic tissues is dynamic and context-dependent, and unable to be precisely captured in a single defined cell culture system. Moreover, mRNA and protein levels of Oct4 and Otx2, the two factors required for reorganization of the enhancer landscape during the naive-to-primed transition (Buecker et al., 2014), are significantly downregulated in Zfp281KO embryos (Figure 2D and Figure 3B–D), which may also explain why Nodal signaling fail to be activated in epiblast maturation (Figure 7). Taken together, our studies highlight the importance of direct in vivo functional investigations using mouse models to refine and/or authenticate in vitro findings of pluripotent state transitions for a better understanding of epiblast maturation.

Nodal signaling has been extensively studied in key sequential events ranging from epiblast maturation to left-right patterning (Shen, 2007). In Nodal mutant embryos, epiblast cells do not mature properly and embryos exhibit a failure in DVE/AVE establishment (Brennan et al., 2001; Mesnard et al., 2006). A recent study suggests that Nodal guides the transition from naive to formative pluripotency in vitro (Mulas et al., 2017). Although pluripotency factors have been reported to occupy and regulate enhancers at the Nodal locus during the ESC-to-EpiSC transition (Papanayotou et al., 2014), how they contribute to lineage specification during the exit from a naive pluripotent state in vivo has remained an open question. Our work reveals that Zfp281 acts as a master regulator of epiblast maturation through the regulation of primed pluripotency genes and Nodal signaling components. Zfp281 binds the PEE, NDE, and HBE Nodal enhancers in vitro and in vivo, with binding at the HBE decreasing during epiblast maturation. In ESCs, HBE is bound by Oct4/Sox2/Nanog/Klf4 (Papanayotou et al., 2014), as well as Zfp281, however neither Zfp281 nor Oct4 binds ASE (Figure 7C), suggesting that loss of Zfp281 binding at HBE may be a prerequisite for decommissioning the naive-specific Nodal enhancer in transitioning to the primed state. In addition, Nodal expression is reduced or not restricted proximally in Zfp281KO embryos, which could further indicate a failure in enhancer switching. Our findings are thus in agreement with the proposed switch of Nodal enhancers, from HBE to ASE, concomitant with the transition from ESCs to EpiSCs (Papanayotou et al., 2014). Together with the crosstalk between Zfp281 and mulitiple histone modifying complexes (PRC2/Ep400/NuRD) (Figure 6), our data have thus uncovered a critical role for the pluripotency factor Zfp281 in coordinating transcriptional and epigenetic control of the exit from naive pluripotency through promoter and enhancer remodeling, leading to activation of lineage-specific genes and genes encoding Nodal signaling components while embryos are progressing to a primed pluripotency state during epiblast maturation (Figure 8).

As the targets and antagonists of Nodal signaling in embryo development, Lefty genes (Lefty1 and Lefty2) are sensitive to status of DNA methylation at promoters. It is reported that ESCs depleted of Tet1 diminished expression of Lefty1 with DNA hyper-methylation at Lefty1 promoter (Koh et al., 2011). Our previous work indicated that Zfp281 physically interacts with Tet1 (Fidalgo et al., 2016), therefore Zfp281 may recruit Tet1 at Lefty1/2 promotors and maintain Lefty expression, suggesting a Nodal signaling-independent role of Zfp281. Although, developmental arrest of Zfp281 mutant embryos (~E6.5) precedes that of Tet-TKO (triple KO) mutants (~E7.5), and defects observed in Tet-TKO mutants were attributed to downregulation of Lefty1/2 through promoter hyper-methylation, leading to constitutive activation of the Nodal signaling pathway due to the disruption of Lefty-mediated negative-feedback (Dai et al., 2016). Therefore, these results raise the question of a possible link between Zfp281 and DNA modification for epigenetic control of proper embryonic development, which is worthy of future investigation.

Materials and methods

Mouse strains, embryo collection and staining

Request a detailed protocol

Mouse strains used in the study were Zfp281KO (Fidalgo et al., 2011) and Hex-GFP (Rodriguez et al., 2001). All mice used in this study were maintained in accordance with the guidelines of the Memorial Sloan Kettering Cancer Center (MSKCC) Institutional Animal Care and Use Committee (IACUC) under protocol number 03-12-017 (PI Hadjantonakis).

Pre-implantation embryos were flushed in M2 medium and processed according to standard protocols (Saiz et al., 2016). Post-implantation embryos were dissected in DMEM/F12 medium supplemented with 5% newborn calf serum and fixed for 20 min in PBS-4% PFA at room temperature for immunohistochemistry or overnight (o/n) at 4°C for wholemount RNA in situ. Following washes in PBS, embryos were permeabilized in PBS-0.5% Triton for 20 min at room temperature, washed in PBS-0.1% Triton (PBT) and blocked at 4°C o/n in PBT-3% BSA. Primary and secondary antibody stainings were performed o/n at 4°C. Counterstaining with Hoechst and fluorophore-coupled phalloidin (Life Technologies, Carlsbad, CA) were performed for 1 hr at room temperature prior to imaging on a Zeiss LSM880 laser scanning confocal microscope. Details of antibodies used in this study can be found below. Fluorescence intensity levels were measured on data acquired with the same imaging parameters. Pre-implantation fluorescence was quantified with our semi-automated MINS 3D nuclear segmentation software (Lou et al., 2014; Saiz et al., 2016). Post-implantation nuclear protein levels were quantified using Imaris software (Bitplane) by manually creating individual nuclear surfaces for each cell and quantifying the fluorescence level inside the volume defined by these surfaces. Fluorescence levels were corrected for fluorescence decay along the z axis. Statistical significance was calculated on the average level of corrected fluorescence per embryo using unpaired two-tailed Student T-test (with Welch's correction when standard deviations differed between samples). Each graph represents either corrected fluorescence values per cell or transformed values using natural log (to represent different protein levels on a similar scale). Primary antibodies used for embryo staining were: Zfp281 (Santa Cruz, sc-166933, 1:200, RRID:AB_10612046), Nanog (Reprocell, RCAB002P-F, 1:500, RRID:AB_2616320), Gata6 (R and D, AF1700, 1:100, RRID:AB_2108901), Brachyury/T (R and D, AF2085, 1:100, RRID:AB_2200235), Oct4 (Santa Cruz, sc-9081X, 1:1000, RRID:AB_2167703), Sox2 (R and D, AF2018, 1:50, RRID:AB_355110), Lhx1 (R and D, MAB2725, 1:250, RRID:AB_2135636), Eomes (Abcam, ab183991, 1:500), Otx2 (R and D, AF1979, 1:1000, RRID:AB_2157172), Oct6 (sc-376143, 1:100, RRID:AB_10989975).

Whole-mount in situ hybridization (WISH)

Request a detailed protocol

Wholemount mRNA in situ hybridizations were performed according to standard protocols (Behringer et al., 2014). The list and citation for each antisense probe used can be found in the following references: Hesx1 (Thomas and Beddington, 1996), Dkk1 (Glinka et al., 1998), CerI (Belo et al., 1997), Hex (Thomas et al., 1998), Lefty1 (Oulad-Abdelghani et al., 1998), Lefty2 (Meno et al., 1997), Nodal (Zhou et al., 1993), Otx2 (Ang and Rossant, 1994), T (Wilkinson et al., 1990), Eomes (Ciruna and Rossant, 1999), Axin2 (Jho et al., 2002), and Fgf8 (Crossley and Martin, 1995).

ESCs and EpiSCs cell culture

Request a detailed protocol

Mouse embryonic stem cells (mESCs) J1 (strain 129S4/SvJae, RRID:CVCL_6412,) and CJ7 (strain 129S1/SvImJ, RRID:CVCL_C316) were cultured on 0.1% gelatin-coated plates in ESM medium: DMEM supplemented with 15% fetal bovine serum (FBS), 1000 units/mL recombinant leukemia inhibitory factor (LIF), 0.1 mM 2-mercaptoethanol, 2 mM L-glutamine, 0.1 mM MEM non-essential amino acids (NEAA), 1% nucleoside mix (100X stock, Sigma), and 50 U/mL Penicillin/Streptomycin). Primed mouse epiblast stem cells (EpiSCs) were culture on fibronectin-coated plates (10 μg/mL/cm2) in N2B27 medium supplemented with Activin A (20 ng/mL) and Fgf2 (12 ng/mL) (Fidalgo et al., 2016). Generation of Zfp281KO ESCs (from CJ7 background, clone no. 2.6 (XX), 3.34 (XY) and 7 (XX)) has been previously described (Fidalgo et al., 2011). All cell lines are from authenticated sources and mycoplasma contamination test was performed routinely.

Tetraploid WT <-> ESCs chimera

Request a detailed protocol

Tetraploid chimeras were generated according to standard protocols (Eakin and Hadjantonakis, 2006; Eggan et al., 2001). Briefly, females were superovulated by i.p. injection of pregnant mares' serum and human chorionic gonadotropin (HCG), and were then mated with males. Fertilized zygotes were isolated from oviducts 24 hr later, cultured until they reached the 2-cell stage, at which point they were electrofused. Fused 1-cell embryos were carefully identified, cultured for another 2 days, and then injected with about fifteen ESCs. ESCs from two Zfp281KO clones 2.6 (XX) and 3.34 (XY), and a Zfp281 cDNA rescued 3.34 clone (XY) were used for tetraploid injection. About 40 ~ 60 injected blastocysts were collected for each ES clone, and transferred into 2 ~ 3 pseudo-pregnant foster females. All experiments involving 2C-embryo electrofusion and tetraploid injection were performed at the Rodent Genetic Engineering Laboratory at New York University, with dissections of resulting post-implantation embryo chimeras taking place at MSKCC.

Knockdown of Zfp281, ALK inhibitor (ALKi) treatment and western blot analysis

Request a detailed protocol

Two shRNAs for Zfp281 knockdown were previously validated in our study (Fidalgo et al., 2016). Lentivirus production and infection were performed as described (Ivanova et al., 2006). Concentrated viral supernatants were incubated with ESCs or EpiSCs for 1 hr, then cells were diluted with fresh medium. Blasticidin (10 µg/mL) was used for selection 24 hr later, and cells were harvested 72 hr after viral infection.

For ALKi treatment, ESCs and EpiSCs were treated with ALK inhibitor A83-01 (1 µM, #2939, Tocris Bioscience) for 48 hr with DMSO as a vehicle control. Western blot analysis was carried out using the following primary antibodies: Zfp281 (sc-166933, Santa Cruz, RRID:AB_10612046), p-Smad2 (#3108, Cell Signaling, RRID:AB_490941), Smad2 (#3103, Cell Signaling, RRID:AB_490816), Lefty (sc-365845, Santa Cruz, RRID:AB_10847353, detecting both Lefty1 and Lefty2), Gapdh (10494–1-AP, ProteinTech, RRID:AB_2263076). Blot intensities were quantified using ImageJ software and Student T-test was used to examine statistical significance.

Induction of primitive streak (PS)-like cells

Request a detailed protocol

ESCs were seeded on fibronectin-coated plates, and treated with Activin A (20 ng/mL) and CHIR99021 (3 µM) for up to 3 days for PS-like cells inductions. Cells were also treated with ALK inhibitor (1 µM) or Zfp281 shRNAs to investigate the effects of Nodal signaling inhibition or Zfp281 knockdown during this differentiation. RNA was collected at different days after treatment.

Co-immunoprecipitation (coIP)

Request a detailed protocol

Two 15 cm dishes containing comparable numbers of confluent ESCs and EpiSCs were harvested, and nuclear extracts were prepared as described previously (Costa et al., 2013). For immunoprecipitation, nuclear extracts of ESCs and EpiSCs were prepared and incubated with pre-bound 4 μg Zfp281 (Abcam, ab101318) or IgG (Millipore, PP64) antibodies with protein G-Agarose beads (#11243233001, Roche) overnight at 4°C. Immunoprecipitates were washed five times with IP buffer, eluted from the beads by boiling, and separated by SDS-PAGE. Western blot analyses were carried out using the following primary antibodies: Zfp281 (sc-166933, Santa Cruz, RRID:AB_10612046), Ep400 (Bethyl, A300-541A, RRID:AB_2098208), Trrap (Santa Cruz, sc-5405, RRID:AB_2209666), Chd4 (Abcam, ab70469, RRID:AB_2229454), Mbd3 (Abcam, ab157464), Suz12 (Abcam, ab12073), Oct4 (Santa Cruz, sc-5279, RRID:AB_628051), and P300 (Santa Cruz, sc-584, RRID:AB_2293429).

RT-qPCR

Request a detailed protocol

Total RNA was extracted from ESCs or EpiSCs using the RNeasy kit (Qiagen), and from E6.5 embryos using the TRIZOL reagent (Ambion, #15596018). Reverse transcription was performed and cDNA was generated using the qScript kit (Quanta, Cat# 95048). Relative expression levels were determined using a LightCycler 480 SYBR green mix (Roche, 4729749001). qRT-PCR experiments were performed on a LightCycler Real Time PCR System (Roche). Gene expression levels were normalized to Gapdh. Error bars indicate standard error for average expression of two technical replicates. Primers for qPCR are listed in Figure 2—source data 2.

ChIP-qPCR

Request a detailed protocol

ChIP assays were performed as described (Lee et al., 2006). Briefly, cells were cross-linked with 1% (w/v) formaldehyde for 10 min at room temperature, and formaldehyde was inactivated by the addition of 125 mM glycine. For embryo ChIP, the protocol was modified. Briefly, embryonic regions were dissected from 24 (Experiment#1) or 25 (Experiment#2) E6.5 WT embryos. After formaldehyde crosslinking and quenched by glycine, embryos were washed once with lysis buffer #1, then resuspend in lysis buffer #3 for sonication. Sonication was performed on a Bioruptor system, with 30 s ON, 30 s OFF, 30 cycles, high amplitude. Chromatin extracts containing DNA fragments were immunoprecipitated by incubating with primary antibody-conjugated DynaBeads (Novex, 10003D) overnight with rotation at 4°C. The immunoprecipitated DNA was purified with ChIP DNA Clean and Concentrator columns (Zymo Research), analyzed by qPCR using Roche SYBR Green reagents and a LightCycler480 machine, and percentage of input recovery was calculated. Error bars indicate standard error for average expression of two technical replicates. The primary antibodies used for ChIP: Zfp281 (Abcam, ab101318, RRID:AB_11157929), H3K27ac (Abcam, ab4729, RRID:AB_2118291), H3K27me3 (Millipore, 07–449, lot 2194165, RRID:AB_310624), Suz12 (Active Motif, #39357, RRID:AB_2614929), and IgG (Millipore, PP64, RRID:AB_97852). The primers used for qPCR are listed in Figure 5—source data 1 and Figure 7—source data 1.

RNA-seq analysis of embryos

Request a detailed protocol

Mouse embryos were dissected at E6.5 and total RNAs were extracted using TRIZOL reagent (Ambion, #15596018) following standard protocols. RNA quality was evaluated with an Agilent 2100 BioAnalyzer system, and embryo genotype was determined by morphology and confirmed by expression of Zfp281 by RT-qPCR. Ten to one-hundred ng total RNA from each embryo was processed for RNA-seq library construction using the Ovation Mouse RNA-seq kit (NuGEN, #0348–32) following the manufacturer’s protocol, then massively parallel sequencing was performed on an Illumina HiSeq 4000 Sequencing System. Between 20 and 50 million 100 bp single-end reads were obtained per sample.

RNA-seq reads were aligned to the genome using TopHat (v2.0.10) and Bowtie2 (v2.1.0) with the default parameter settings. UCSC mm9 mouse genome, as well as the transcript annotation, was downloaded from the iGenomes site. Transcript assembly and differential expression analyses were performed using Cufflinks (v2.1.1). Assembly of novel transcripts was not allowed (-G), other parameters of Cufflinks followed the default setting. The summed RPKM (reads per kilobase per million mapped reads) of transcripts sharing each gene_id were calculated and exported by the Cuffdiff program. In the RPKM data matrix, a minimal RPKM value of 0.1 was applied if gene expression was less than this minimal value. P-values were calculated using T-test.

RNA-seq data of WT and Zfp281KO ESCs and EpiLCs from our previous study (accession: GSE81042) were processed with the same settings.

Gene Ontology (GO) analysis

Request a detailed protocol

Gene ontology analyses were performed using the DAVID gene ontology functional annotation tool (http://david.abcc.ncifcrf.gov/tools.jsp) with all NCBI Mus musculus genes as a reference list.

Geneset enrichment analysis (GSEA)

Request a detailed protocol

GSEA (v2.1.0, available at http://www.broadinstitute.org/gsea) was used to determine whether the set of genes of interest was statistically enriched in Zfp281KO versus WT embryo RNA-seq data. The genesets used for this study were derived from the Reactome pathway database. The enrichment plot, normalized enrichment score (NES) and q-value (FDR) were indicated for each enrichment test.

ChIP-seq analysis

Request a detailed protocol

ChIP was performed as previously described (Lee et al., 2006) using a Zfp281 antibody (Abcam, ab101318) in EpiSCs. ChIP-seq data of Zfp281 in ESCs were from our previous data set at GEO (accession: GSE81042). Massively parallel sequencing was performed with the Illumina HiSeq 2500 Sequencing System according to the manufacturer’s protocol. All libraries were sequenced as 100 bp single-end reads. ChIP-seq raw data were processed as previously described (Fidalgo et al., 2016). Briefly, reads were aligned to the mouse genome (NCBI build 37, mm9) using the Bowtie (v1.0.0) program, with parameters -m 1. The aligned reads were sorted, and duplicated reads were removed using samtools (v0.1.19). BAM files were converted to a binary tiled file (tdf), and visualized using IGV (v2.3) software.

Zfp281 ChIP-seq peaks were determined using the MACS2 program (v2.0.10) with input ChIP-seq as the control. MACS2 parameters followed the default settings. Zfp281 ChIP-seq peaks were annotated using the annotatePeaks module in the HOMER program (v4.6) against the mm9 genome. Overlap of Zfp281 ChIP-seq peaks in ESCs and EpiSCs was determined by Bedtools (v2.18.1). The diffbind package (available from http://bioconductor.org/, v1.16.3) was used to determine the ChIP-seq intensities (by aligned reads per million total reads, RBM) of Zfp281 peaks.

Public ChIP-seq data were downloaded from GEO, and processed with the same settings. Colocalization of Zfp281 and other transcription and epigenetic factors (see accessions in Figure 6—source data 1) was performed with an in-house Python (v2.7.6) program as previously described (Ding et al., 2015). Briefly, ChIP-seq reads were downloaded and processed to determine a peak list by MACS2. A phi correlation coefficient was used to calculate the correlation between the peak lists of every two ChIP-seq data. Heatmap of correlations was shown with the TreeView program.

Accession number

Request a detailed protocol

Zfp281 ChIP-seq data and Zfp281KO embryo RNA-seq data are available in GEO under accession GSE93044.

Data availability

The following data sets were generated
The following previously published data sets were used

References

    1. Ang SL
    2. Jin O
    3. Rhinn M
    4. Daigle N
    5. Stevenson L
    6. Rossant J
    (1996)
    A targeted mouse Otx2 mutation leads to severe defects in gastrulation and formation of axial mesoderm and to deletion of rostral brain
    Development 122:243–252.
    1. Behringer R
    2. Gertsenstein M
    3. Nagy K
    4. Nagy A
    (2014)
    Manipulating the Mouse Embryo: A Laboratory Manual
    Manipulating the Mouse Embryo: A Laboratory Manual.
    1. Crossley PH
    2. Martin GR
    (1995)
    The mouse Fgf8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo
    Development 121:439–451.
    1. Kalkan T
    2. Smith A
    (2014) Mapping the route from naive pluripotency to lineage specification
    Philosophical Transactions of the Royal Society B: Biological Sciences 369:20130540.
    https://doi.org/10.1098/rstb.2013.0540
    1. Oulad-Abdelghani M
    2. Chazaud C
    3. Bouillet P
    4. Mattei MG
    5. Dollé P
    6. Chambon P
    (1998)
    Stra3/lefty, a retinoic acid-inducible novel member of the transforming growth factor-beta superfamily
    The International Journal of Developmental Biology 42:23–32.
    1. Thomas PQ
    2. Brown A
    3. Beddington RS
    (1998)
    Hex: a homeobox gene revealing peri-implantation asymmetry in the mouse embryo and an early transient marker of endothelial cell precursors
    Development 125:85–94.

Article and author information

Author details

  1. Xin Huang

    1. The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, United States
    2. Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States
    Contribution
    Conceptualization, Resources, Data curation, Methodology, Writing—original draft, Writing—review and editing
    Contributed equally with
    Sophie Balmer
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-6778-8849

  2. Sophie Balmer

    Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, United States
    Contribution
    Conceptualization, Data curation, Methodology, Writing—original draft, Writing—review and editing
    Contributed equally with
    Xin Huang
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6561-552X

  3. Fan Yang

    1. The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, United States
    2. Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States
    3. Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University, Yangling, China
    Contribution
    Methodology
    Competing interests
    No competing interests declared

  4. Miguel Fidalgo

    1. The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, United States
    2. Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States
    3. Departamento de Fisioloxia, Centro de Investigacion en Medicina Molecular e Enfermidades Cronicas, Universidade de Santiago de Compostela, Santiago, Spain
    Contribution
    Methodology
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1134-2674

  5. Dan Li

    1. The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, United States
    2. Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States
    3. The Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, United States
    Contribution
    Methodology
    Competing interests
    No competing interests declared

  6. Diana Guallar

    1. The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, United States
    2. Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States
    Contribution
    Methodology
    Competing interests
    No competing interests declared

  7. Anna-Katerina Hadjantonakis

    Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, United States
    Contribution
    Conceptualization, Writing—original draft, Project administration, Writing—review and editing
    For correspondence
    hadj@mskcc.org
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7580-5124

  8. Jianlong Wang

    1. The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, United States
    2. Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States
    3. The Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, United States
    Contribution
    Conceptualization, Writing—original draft, Project administration, Writing—review and editing
    For correspondence
    jianlong.wang@mssm.edu
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1317-6457

Funding

National Institutes of Health (R01-GM095942)

  • Jianlong Wang

National Institutes of Health (R21-HD087722)

  • Jianlong Wang

New York State Department of Health (C028103)

  • Jianlong Wang

New York State Department of Health (C028121)

  • Jianlong Wang

National Institutes of Health (R01-DK084391)

  • Anna-Katerina Hadjantonakis

National Institutes of Health (P30-CA008748)

  • Anna-Katerina Hadjantonakis

New York State Department of Health (C029568)

  • Anna-Katerina Hadjantonakis

The Irma T. Hirschl/Monique Weill-Caulier Trust (Career Scientist Award)

  • Jianlong Wang

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Dr. SY Kim and the Rodent Genetic Engineering Laboratory at NYU for the generation of tetraploid embryo chimeras; Drs. M Goll, S Nowotschin, X Shen, C Simon, P Soriano and T Wu for critical reading of the manuscript. This research was funded by grants from the National Institutes of Health (NIH) to JW (R01-GM095942 and R21-HD087722), the Empire State Stem Cell Fund through New York State Department of Health (NYSTEM) to JW (C028103, C028121). JW is a recipient of Irma T Hirschl and Weill-Caulier Trusts Career Scientist Award. Work in A-KH’s lab is funded by NYSTEM (C029568) and the NIH (R01-DK084391 and P30-CA008748).

Ethics

Animal experimentation: All mice used in this study were maintained in accordance with the guidelines of the Memorial Sloan Kettering Cancer Center (MSKCC) Institutional Animal Care and Use Committee (IACUC) under protocol number 03-12-017 (PI Hadjantonakis).

Version history

  1. Received: November 6, 2017
  2. Accepted: November 17, 2017
  3. Accepted Manuscript published: November 23, 2017 (version 1)
  4. Version of Record published: November 30, 2017 (version 2)

Copyright

© 2017, Huang et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 3,203
    views
  • 492
    downloads
  • 25
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Xin Huang
  2. Sophie Balmer
  3. Fan Yang
  4. Miguel Fidalgo
  5. Dan Li
  6. Diana Guallar
  7. Anna-Katerina Hadjantonakis
  8. Jianlong Wang
(2017)
Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling
eLife 6:e33333.
https://doi.org/10.7554/eLife.33333

Share this article

https://doi.org/10.7554/eLife.33333

Categories and tags

Research organism

Further reading

    1. Developmental Biology
    2. Structural Biology and Molecular Biophysics

    Deep learning insights into the architecture of the mammalian egg-sperm fusion synapse

    Arne Elofsson, Ling Han ... Luca Jovine
    Research Article

    A crucial event in sexual reproduction is when haploid sperm and egg fuse to form a new diploid organism at fertilization. In mammals, direct interaction between egg JUNO and sperm IZUMO1 mediates gamete membrane adhesion, yet their role in fusion remains enigmatic. We used AlphaFold to predict the structure of other extracellular proteins essential for fertilization to determine if they could form a complex that may mediate fusion. We first identified TMEM81, whose gene is expressed by mouse and human spermatids, as a protein having structural homologies with both IZUMO1 and another sperm molecule essential for gamete fusion, SPACA6. Using a set of proteins known to be important for fertilization and TMEM81, we then systematically searched for predicted binary interactions using an unguided approach and identified a pentameric complex involving sperm IZUMO1, SPACA6, TMEM81 and egg JUNO, CD9. This complex is structurally consistent with both the expected topology on opposing gamete membranes and the location of predicted N-glycans not modeled by AlphaFold-Multimer, suggesting that its components could organize into a synapse-like assembly at the point of fusion. Finally, the structural modeling approach described here could be more generally useful to gain insights into transient protein complexes difficult to detect experimentally.

    1. Developmental Biology

    Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis

    Amandine Jarysta, Abigail LD Tadenev ... Basile Tarchini
    Research Article

    Inhibitory G alpha (GNAI or Gαi) proteins are critical for the polarized morphogenesis of sensory hair cells and for hearing. The extent and nature of their actual contributions remains unclear, however, as previous studies did not investigate all GNAI proteins and included non-physiological approaches. Pertussis toxin can downregulate functionally redundant GNAI1, GNAI2, GNAI3, and GNAO proteins, but may also induce unrelated defects. Here, we directly and systematically determine the role(s) of each individual GNAI protein in mouse auditory hair cells. GNAI2 and GNAI3 are similarly polarized at the hair cell apex with their binding partner G protein signaling modulator 2 (GPSM2), whereas GNAI1 and GNAO are not detected. In Gnai3 mutants, GNAI2 progressively fails to fully occupy the sub-cellular compartments where GNAI3 is missing. In contrast, GNAI3 can fully compensate for the loss of GNAI2 and is essential for hair bundle morphogenesis and auditory function. Simultaneous inactivation of Gnai2 and Gnai3 recapitulates for the first time two distinct types of defects only observed so far with pertussis toxin: (1) a delay or failure of the basal body to migrate off-center in prospective hair cells, and (2) a reversal in the orientation of some hair cell types. We conclude that GNAI proteins are critical for hair cells to break planar symmetry and to orient properly before GNAI2/3 regulate hair bundle morphogenesis with GPSM2.

    1. Computational and Systems Biology
    2. Developmental Biology

    A logic-incorporated gene regulatory network deciphers principles in cell fate decisions

    Gang Xue, Xiaoyi Zhang ... Zhiyuan Li
    Research Article

    Organisms utilize gene regulatory networks (GRN) to make fate decisions, but the regulatory mechanisms of transcription factors (TF) in GRNs are exceedingly intricate. A longstanding question in this field is how these tangled interactions synergistically contribute to decision-making procedures. To comprehensively understand the role of regulatory logic in cell fate decisions, we constructed a logic-incorporated GRN model and examined its behavior under two distinct driving forces (noise-driven and signal-driven). Under the noise-driven mode, we distilled the relationship among fate bias, regulatory logic, and noise profile. Under the signal-driven mode, we bridged regulatory logic and progression-accuracy trade-off, and uncovered distinctive trajectories of reprogramming influenced by logic motifs. In differentiation, we characterized a special logic-dependent priming stage by the solution landscape. Finally, we applied our findings to decipher three biological instances: hematopoiesis, embryogenesis, and trans-differentiation. Orthogonal to the classical analysis of expression profile, we harnessed noise patterns to construct the GRN corresponding to fate transition. Our work presents a generalizable framework for top-down fate-decision studies and a practical approach to the taxonomy of cell fate decisions.