食品科学

• 生物工程 • 上一篇    下一篇

高产DHA寇氏隐甲藻突变株的筛选

佘 隽1,2,田 华1,陈 涛3,何东平1,2,*   

  1. 1.武汉轻工大学食品科学与工程学院,湖北 武汉 430023;2.湖北百信食品有限公司研发中心,湖北 武汉 430040;
    3.中国科学院武汉病毒研究所,湖北 武汉 430071
  • 出版日期:2013-09-15 发布日期:2013-09-27
  • 通讯作者: 何东平

Screening of High-yield DHA-producing Crypthecodium cohnii Mutant

SHE Jun1,2,TIAN Hua1,CHEN Tao3,HE Dong-ping1,2,*   

  1. 1. College of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China;2. Hubei Pashun Foods
    Co. Ltd., Wuhan 430040, China;3. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: HE Dong-ping

摘要:

以寇氏隐甲藻ATCC30772(C. cohnii ATCC30772)为出发菌株,经60Co-γ射线辐照诱变,根据生长发育、苏丹黑B染色镜检、生物量、含油率和DHA含量等指标,经初筛、复筛,选出最佳突变株2.4k-2A2-5,其平均DHA含量达到39.04%,比出发株提高了3.39%,油脂产量和含油率比出发株分别提高了51.66%和63.37%,其DHA产量为7.14g/L发酵液,比出发株提高了近56.92%。

关键词: 寇式隐甲藻, 60Co-&gamma, 射线, DHA

Abstract:

The original strain C. cohnii ATCC 30772 was irradiated by 60Co-γ ray. Mutant 2.4k-2A2-5 was selected as a
desired positive mutant according to its growth and development, Sudan black B staining, biomass, oil content and DHA
content, following screening and rescreening. The average DHA production was 39.04%, which was 3.39% higher than the
original strain. The yield and content of oil produced by this mutant was 51.66% and 63.37%, respectively, which were also
higher than those by the original strain. The DHA yield (measured in 7.14 g/L fermented liquid) was increased by 56.92%
compared to that produced by the original strain.

Key words: Crypthecodium cohnii;60Co-&gamma, rays;DHA

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