目的 评价不同批次虎杖饮片一致性与差异性,为其质量控制提供方法参考。方法 采用HPLC法建立11批虎杖饮片的化学指纹图谱;采用总量统计矩法计算总量零阶矩(AUC_T)、总量响应率(AUCPW_T)、总量一阶矩(MCRT_T)、总量二阶矩(VCRT_T)等参数;采用总量统计矩法、夹角余弦法和相关系数法进行相似度评价;采用聚类分析法与主成分分析(PCA)法对虎杖样品进行分类分析。结果 对11批虎杖饮片进行测定,共标定了13个共有峰,指认了虎杖苷、白藜芦醇、大黄素苷、大黄素的色谱峰;总量统计矩参数AUC_T、AUCPW_T、MCRT_T、VCRT_T的平均值分别为1.52×10⁶ AU·min、2.31×10³ AU·min·mL/mg、22.0 min、110.0 min²;各批次样品的相似度在0.85以上;聚类分析法与PCA法均将样品分为4类。结论 化学指纹图谱结合总量统计矩分析、聚类分析与PCA可评价虎杖饮片一致性与差异性,是适合中药特点的质量控制方法。

Objective To establish a method to evaluate the consistency and difference of Polygoni Cuspidati Rhizoma et Radix from different batches. Methods Chemical fingerprints of 11 batches of Polygoni Cuspidati Rhizoma et Radix were established by HPLC; The follow parameters, such as under curve of total quantum (AUC_T), area under curve of total quantum perweight (AUCPW_T), mean chromatographic retention time of total quantum (MCRT_T), and variance of chromatographic retention time of total quantum (VCRT_T), were also calculated using total statistical moment method. The similarities were obtained by total statistical moment method, cosine method, and correlation coefficient method. Cluster analysis (CA) and principal component analysis (PCA) were adopted to classify the samples. Results Chemical fingerprints were established with 13 common peaks and polydatin, resveratrol, emodin glycosides, and emodin were identified. AUC_T, AUCPW_T, MCRT_T, and VCRT_Twere calculated as 1.52×10⁶ AU·min, 2.31×10³ AU·min·mL/mg, 22.0 min, and 110.0 min², respectively. The similarities of all batches of samples were over 0.85. The samples were divided into four categories by CA or PCA. Conclusion Chemical fingerprint combined with total statistical moment method, CA, and PCA can evaluate the consistency and difference of Polygoni Cuspidati Rhizoma et Radix, which is suitable for the quality control of Chinese materia medica.

国家自然科学基金面上项目(81173194);湖南省中药活性物质筛选工程技术研究中心项目资助(湘科计字[2013]83号);湖南省高校"中药新药创制与资源综合持续利用"科技创新团队资助(湘教通[2010]212号);湖南省"中药学"重点学科资助(湘教通[2011]76号);国家级大学生创新创业训练计划项目(201410538015);湖南省大学生研究性学习与创新性实验计划项目(2014194);中南林业科技大学大学生研究性学习与创新性实验计划项目(201457)