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Original Article
Changes in Antimicrobial Susceptibility Pattern of Blood Isolates at a University Hospital in the Kyungnam area during 2005-2014
Dahae Yang, Woonhyoung Lee
Kosin Medical Journal 2018;33(1):29-40.
DOI: https://doi.org/10.7180/kmj.2018.33.1.29
Published online: January 21, 2018

Department of Laboratory Medicine, College of Medicine, Kosin University Busan, Korea

Corresponding Author: Woonhyoung Lee, Department of Laboratory Medicine, College of Medicine, Kosin University, 262, Gamchen-ro, Seo-gu, Busan 49267, Korea Tel: +82-51-990-6373 Fax: +82-51-990-3034 E-mail: lukerubicon@gmail.com
• Received: January 12, 2017   • Accepted: March 15, 2017

Copyright © 2018 Kosin University School of Medicine Proceedings

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Objectives
    Blood culture is a one of the most important procedure for diagnosis and treatment of infectious disease, but distribution of pathogenic species and the antimicrobial susceptibility can be vary from pathogen, individual trait, regional or environmental features. In this study, we investigated the changes in frequency of occurrence and antimicrobial susceptibility pattern of blood isolates from 2005 to 2014.
  • Methods
    Data of blood isolates from Kosin Gospel Hospital during 2005 to 2014 were analyzed retrospectively. Blood isolates were cultured for 5 days using BACTEC Plus Aerobic/F and BACTEC lytic/10 Anaerobic/F. Identification and antimicrobial susceptibility test was performed using VITEK 1 system, VITEK 2 XL, PHOENIX 100 and conventional method.
  • Results
    9,847 isolates were identified during 10 years. Among the isolates aerobic or falcutative anaerobic bacteria were isolated in 99.5% specimens, anaerobic were 0.1%, and fugi were 0.4%. Most commonly isolated bacteria were coagulase-negative Staphylococcus (CoNS) followed by Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae. Candida parapsilosis were most frequently isolated among fungi. The proportion of S. aureus, A. baumannii and E. faecium were increased, while Pseudomonas aeruginosa and Streptococcus pneumoniae decreased over decennium. Imipenem resistant K. pneumoniae were identified. Vancomycin resistant E. faecium and imipenem resistant A. baumannii were increased (7.1% in 2005 to 12.3% in 2014, 0% in 2005 to 55.6% in 2014, respectively).
  • Conclusions
    Over the last 10 year, CoNS were the most frequently isolated pathogen. Imipenem resistant K. pneumoniae was emerged. Vancomycin resistant E. faecium and imipenem resistant A. baumannii increased during this period.
Fig. 1.
Annual isolation of relatively common species of bacteria during 2005-2014
kmj-33-29f1.jpg
Fig. 2.
Trends in antimicrobial resistance (%) of (A) S. aureus, (B) E. faecalis, (C) E. faecium, (D) E. coli (E) K. pneumonia (F) P. aeruginosa and (G) A. baumannii by year.
kmj-33-29f2.jpg
Table 1.
Microorganisms isolated by year
Oreauisin No. of isolate according to years
2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 Tola] (%)
Gram-positive Cocci 239 349 372 566 584 748 699 674 614 563 5408 (54.9)
Gram-negativ cocci 1 0 1 1 1 0 0 0 1 0 5 (0.1)
Garm-positive bacilli 7 22 24 2 1 4 2 8 3 3 76 (0.8)
Gram-negativ bacilli 269 284 325 386 409 436 518 526 563 587 4303 (43.7)
Abaerobic 0 0 0 0 0 1 3 3 3 1 11 (0.1)
Fungi 0 1 4 5 5 8 8 6 6 4 44 (0.4)
Tolal 516 656 726 960 1000 1197 1227 1217 1190 1158 9847 (100.0)
Table 2.
Distribution of blood isolates
Organisms No. ofisolates %
Gram positive organisms
Staphylococcus, coagulase-negative 2761 28.0
Staphylococcus aureus 1074 10.9
α- hemolytic Staphylococcus 502 5.1
Entero coccus faecium 392 4.0
Enterococcus faecalis 238 2.4
Streptococcus pneumoniae 136 1.4
Bacillus spp. 4.5 0.5
Corym bacterium spp. 29 0.3
Other gram positive 307 3.1
Gram negative organisms
Escherichia coli 2078 21.1
Klebsiella pneumoniae 857 3.7
Enterobacter spp. 273 2.3
Acinetobacter baumannii 225 2.3
Pseudomonas aeruginosa 211 2.1
Serratia spp. 74 0.3
Aemmonas spp. 73 0.7
Stenotrqphomonas maltophilia 72 0.7
Other gram negative 445 4.5
Anaerobic organi ans
Lactococcus garvieae 1 <0.1
Propionibacterium acnes 8 0.1
Bacteroides fragilis 1 <0.1
Chromo bacterium violaceum 1 <0.1
Fungal organisms
Candida albicans 8 0.1
Candida tropicalis 3 <0.1
Candida glabrata 8 0.1
Candidaparaps “dosis 17 0.2
Other Yeast 8 0.1
Total 9847 100.0
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