Two new antibiotics, iturin D and iturin E, were isolated from a strain of Bacillus subtilis producing iturin A. These compounds belong to the iturin group, the acid hydrolysates contained α-amino acids Asp₃, Glu₁, Pro₁, Ser₁ Tyr₁ and a mixture of n-C₁₄, iso-C₁₅, anteiso-C₁₅, iso-C₁₆ and n-C₁₆ β-amino acids.
They differ from iturin A by the presence of a free carboxyl group in iturin D and a carboxymethyl group in iturin E.