In previous studies, we found that overexpression ADH3p in BJ2168 will let mitochondria fission. BJ2168 is protease-deficient stain. In order to verify the result in BY4742 and ΔADH3(BY4741) strain, we did the same experiments to verify the results. Finally, in BY4742 strain, it had the same result. But in ADH3Δstrain, it looked different. It might be due to insufficient amount ADH3p.