The effects of membrane morphology on the membrane blocking and particle fouling in a “dead-end” microfiltration system are studied by use of membrane blocking models. Three kinds of membranes, Isopore, Durapore, and MF-Millipore membranes manufactured by Millipore Company in USA, with two mean pore sizes are used for filtering 0.15μm PMMA particles in experiments. The values of blocking index and resistance coefficient during filtration under various operating conditions are analyzed and discussed. The membrane blocking charts are established for relating the blocking index, filtration rate, and particle accumulation for the used membranes. The resistance coefficients obtained under various conditions can be correlated to a unique function of blocking index. Different modes of membrane blockings occur in the early period of a filtration, the blocking index decreases continuously during that period. The blocking index then suddenly drops to zero at a critical point, and the filtration follows the cake filtration model henceforward. The experimental data show that MF-Millipore membrane is the easiest to be blocked under a fixed filtration rate. However, Isopore is also easier to be blocked than Durapore membrane due to its low pore density. In addition, Isopore membrane has the highest blocking index while MF-Millipore membrane has the lowest under a given particle accumulation. To compare the critical conditions of three kinds of membranes, the critical condition of MF-Millipore membrane occurs at the lowest particle accumulation and the highest filtration rate, however, the critical condition of Isopore membrane occurs at the highest particle accumulation and the lowest filtration rate. Those results are due to different membrane surface morphologies and membrane pore structures.