Catheter-associated urinary tract infection (CAUTI) increases health care costs and the lengths of hospital stays. The incidence density of the CAUTI at our unit in 2013 was 5.1 per mille which was higher than the average (3.9 per mille) of all surgical intensive care units. Therefore, this project aimed to decrease the incidence density to below 3.9 per mille. The causes of the high incidence density are the (1) incorrect cognition of CAUTI, (2) incorrect nursing skills of urinary catheter insertion and perineal care, (3) lack of the indications of using catheterization, (4) lack of developing standards of nursing care for urinary catheter maintenance, and (5) the insufficient equipment of glove replacement. After establishing the in-service education, the technical audit, indications of using catheterization, and the standard of nursing skills, the incidence densities of CAUTI decreased from 5.1 per mille to 3.5 per mille. This project effectively increased the nurses' knowledge about CAUTI and improved quality of nursing care and patient safety.