Expression of RANTES and CCR5 in Rats with Experimental Autoimmune Anterior Uveitis

目的：急性前虹彩炎是人類最常見的虹彩炎，也是造成視力不良的重要原因之一。目前對於虹彩炎的病因及致病機轉並不清楚。因此在治療方面只能用非特異性類固醇來做症狀的治療。但是類固醇只能暫時壓制發炎反應。病人還是會反復發作，並且會產生一些嚴重的副作用。RANTES是一種細胞激素，主要與T淋巴球與單核球的聚集及浸潤有很大的相關。目前為止證實很多自體免疫疾病與RANTES有很大的相關。本研究藉由探討一種與人類急性前虹彩炎類似動物模式實驗性自體免疫前房虹彩炎的致病機轉，研究RANTES和其受體所扮演的角色，來更加瞭解人類急性前虹彩炎的致病機制及治療方式。方法：先在大鼠腳底板打入牛黑色素相關搞原引發實驗性自體免疫前房虹彩炎。在不同時間點上萃取出鼠虹彩及睫狀體的RNA，設計RANTES和CCR5引子進行反轉錄和聚合鍊鎖反酶。並且在不同時間點上測量房水內RANTES蛋白質濃度。結果：RANTES mRNA和蛋白質在注射抗原後第十一天開始升高，和臨床開始發病及白血球開始聚集的時間相同，並隨著臨床疾病減弱而降低表現。此外CCR5也同樣在第十一天開始表現。結論：RANTES在實驗性自體免疫前房虹彩炎的致病機轉中扮演重要角色。抗RANTES治療似乎可成爲將來治療人類急性前虹彩炎的重要方法。

關鍵字

自體免疫前虹彩炎；化學激素； RANTES ；化學激素受體

並列摘要

Objective: To determine the pattern of expression of RANTES and its receptor, CCR5, and to establish their roles in experimental autoimmune anterior uveitis, an animal model of human acute anterior uveitis. Methods: Uveitis was induced in the Lewis rats with the injection melanin-associated antigen into the left footpad. The rats were scored for the development of clinical disease. At defined tine points, RANTES and CCR5 mRNA expression were semiquantified by using RT-PCR. The concentration of RANTES protein in aqueous humor was quantified by ELSIA. Results: RANTES mRNA started increasing on day 11, concurrent with the infiltration of leukocytes in aqueous humor and onset of clinical disease. The concurrent with the infiltration of leukocytes in aqueous humor and onset of clinical disease. The concentration of RANTES protein also increased on day 11, Furthermore in accord with its ligand RANTES contributed to the recruitment of inflammatory cells into the eye and to the disease activity. RANTES could be potential targets for acute anterior uveitis therapy.

並列關鍵字

RANTES ； CCR5 ； chemokine ； chemokine ； receptor ； uveitis

國際替代計量

Expression of RANTES and CCR5 in Rats with Experimental Autoimmune Anterior Uveitis

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