The incidence of cancer continues to increase worldwide. Prevention is the key to effectively controlling the rising number of cancer patients. Although the Taiwanese government has provided medical subsidies for four specific types of cancer treatment modalities, the screening rate is still low. Therefore, researchers were motivated to determine methods to enhance the persuasive effects of cancer guidance messages. The study explored the persuasive effects of posters and evaluated the appropriateness of the information being presented by analysing the framing of the message and the method of information presentation, which affect people's willingness to participate in cancer screening. This study targeted people aged 20 years or older. It adopted a convenient sampling method and questionnaire survey procedure. The researchers distributed 469 questionnaires, of which 436 were valid. Subsequently, they employed two-way ANOVA to analyse the information collected. The major findings of this health communication study are as follows: (1) loss-framed messages have a stronger persuasive effect than gain-framed messages do, (2) the persuasive effect of the conscience-raising method is stronger than those of the other approaches, and (3) message framing and information presentation are interrelated. The results of this study may serve as an avenue for future research and design practices.