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CC BY-NC 4.0 · Arch Plast Surg 2013; 40(05): 496-504
DOI: 10.5999/aps.2013.40.5.496
Original Article

Effect of Amniotic Fluid Stem Cells and Amniotic Fluid Cells on the Wound Healing Process in a White Rat Model

Jung Dug Yang
1Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, Korea
,
Dong Sik Choi
1Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, Korea
,
Young Kyoo Cho
1Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, Korea
,
Taek Kyun Kim
1Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, Korea
,
Jeong Woo Lee
1Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, Korea
,
Kang Young Choi
1Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, Korea
,
Ho Yun Chung
1Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, Korea
,
Byung Chae Cho
1Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, Korea
,
Jin Suk Byun
KBS Aesthetic Clinic, Daegu, Korea
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Background Amniotic-fluid-derived stem cells and amniocytes have recently been determined to have wound healing effects, but their mechanism is not yet clearly understood. In this study, the effects of amniotic fluid stem cells and amniocytes on wound healing were investigated through animal experiments.

Methods On the back of Sprague-Dawley rats, four circular full-thickness skin wounds 2 cm in diameter were created. The wounds were classified into the following four types: a control group using Tegaderm disc wound dressings and experimental groups using collagen discs, amniotic fluid stem cell discs, and amniocyte discs. The wounds were assessed through macroscopic histological examination and immunohistochemistry over a period of time.

Results The amniotic fluid stem cell and amniocyte groups showed higher wound healing rates compared with the control group; histologically, the inflammatory cell invasion disappeared more quickly in these groups, and there was more significant angiogenesis. In particular, these groups had significant promotion of epithelial cell reproduction, collagen fiber formation, and angiogenesis during the initial 10 days of the wound healing process. The potency of transforming growth factor-β and fibronectin in the experimental group was much greater than that in the control group in the early stage of the wound healing process. In later stages, however, no significant difference was observed.

Conclusions The amniotic fluid stem cells and amniocytes were confirmed to have accelerated the inflammatory stage to contribute to an enhanced cure rate and shortened wound healing period. Therefore, they hold promise as wound treatment agents.

Keywords

Amniotic fluid - Stem cells - Wound healing

Supplemental Fig. S1.

    Serial gross photos of the rat’s wound.

    (A) Four days after application. (B) Six days after application. (C) Ten days after application.

    (D) Fourteen days after application. (E) Seventeen days after application. (F) Twenty-one days after application.

    Supplemental data can be found at: http://e-aps.org/src/sm/aps-40-496-s001.pdf

  • Supplementary material


Publication History

Received: 22 April 2013

Accepted: 27 July 2013

Article published online:
01 May 2022

© 2013. The Korean Society of Plastic and Reconstructive Surgeons. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonCommercial License, permitting unrestricted noncommercial use, distribution, and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes. (https://creativecommons.org/licenses/by-nc/4.0/)

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