Applied Science and Convergence Technology

The Korean Vacuum Society (한국진공학회)
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A Portable Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella typhimurium

  • Nguyen, Hoang Hiep (BioNanotechnology Research Center) ;
  • Yi, So Yeon (BioNano H-Guard Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Woubit, Abdela (Department of Pathobiology, College of Veterinary Medicine Nursing & Allied Health (CVMNAH), Tuskegee University) ;
  • Kim, Moonil (BioNanotechnology Research Center)
  • Received : 2016.05.15
  • Accepted : 2016.05.30
  • Published : 2016.05.30
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Abstract

Here, the rapid detection of Salmonella typhimurium by a portable surface plasmon resonance (SPR) biosensor in which the beam from a diode laser is modulated by a rotating mirror is reported. Using this system, immunoassay based on lipopolysaccharides (LPS)-specific monoclonal anti-Salmonella antibody was performed. For the purpose of orientation-controlled immobilization of antibodies on the SPR chip surface, the cysteine-mediated immobilization method, which is based on interaction between a gold surface and a thiol group (-SH) of cysteine, was adopted. As a result, using the portable SPR-based immunoassay, we detected S. typhimurium in the range from 10^7 CFU/mL to 10^9 CFU/mL within 1 hour. The results indicate that the portable SPR system could be potentially applied for general laboratory detection as well as on-site monitoring of foodborne, clinical, and environmental agents of interest.

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