Secondary Metabolites of an of Streptomyces griseorubens Isolate Are Predominantly Pyrrole- and Linoleic-acid like Compounds

Serap Çetinkaya; Ali Fazıl Yenidünya; Kubilay Arslan; Dönsel Arslan; Özgül Doğan; Taner Daştan

doi:10.5650/jos.ess20161

Biochemistry and Biotechnology

Secondary Metabolites of an of Streptomyces griseorubens Isolate Are Predominantly Pyrrole- and Linoleic-acid like Compounds

Serap Çetinkaya, Ali Fazıl Yenidünya, Kubilay Arslan, Dönsel Arslan, Özgül Doğan, Taner Daştan

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Keywords: erucic acid, linoleic acid, pyrrolo[1,2-a]pyrazine-1,4-dione hexahydro, Streptomyces griseorubens

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Supplementary material

2020 Volume 69 Issue 10 Pages 1273-1280

DOI https://doi.org/10.5650/jos.ess20161

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Abstract

The study involved the isolation and identification of a member of Streptomyces griseorubens and the identification of its secondary metabolite content. Two extract samples were prepared by using butanol and chloroform. In the analyses of the extracts TLC, FT-IR, and GC-MS were employed. Butanol extract appeared to be dominated by three different pyrrole compounds (43.59%), while two fatty acids, linoleic- and erucic acids, were the most abundant secondary metabolites in the chloroform extract, 27.57% and 12.34%, respectively. Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-compound was represented by a single and distinct band on the thin layer chromatography plate. In GC-MS spectra, it also constituted 13.50% of the butanol extract.

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