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Overexpression of Rictor protein and Rictor-H. pylori interaction has impact on tumor progression and prognosis in patients with gastric cancer
, 1, 2, 1, 3, 1, 1, 4, 4, 1, 1, 5, 16, 1
Affiliations- Department of Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China
- Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei 230032, Anhui, China
- Department of Pharmacy, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, Hefei, China
- Department of Pathology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China
- Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032, Anhui, China
- Institute for Liver Diseases of Anhui Medical University, Hefei 230032, Anhui, Hefei, China
open access
Abstract
Introduction. Growing evidence indicates that Rictor (Rapamycin-insensitive companion of mTOR) is overexpressed across several malignancies and associated with poor survival. However, only limited data indicate that Rictor plays a role in gastric cancer (GC). We sought to explore the prognostic value of Rictor in GC and present interaction analysis between Rictor expression and H. pylori status regarding their effects over the prognosis of GC patient.
Materials and methods. 250 GC tissues and 124 lymph node metastases were collected for the detection of Rictor by immunohistochemistry. Cox regression model was used to assess the association between Rictor expression and patient prognosis. Functional experiments were examined in transfected cells using Rictor siRNA. Additive and multiplicative interactions of Rictor and H. pylori were evaluated.
Results. In this study, the positive rate of Rictor was 51.6% (129/150) in GC tissues. Multivariate analyses showed that Rictor was independent unfavorable predictor for OS (HR = 1.554, 95% CI = 1.076–2.244, P = 0.019) and DFS (HR = 1.556, 95% CI = 1.081–2.240, P = 0.017). Patients with upregulated Rictor in the primary tumor and lymph node metastases had the worst prognosis. We observed significant additive and multiplicative interactions between Rictor expression and H. pylori status for OS and DFS (P < 0.05). Our in vitro experiment showed that knockdown of Rictor could suppress cell proliferation, induce apoptosis and inhibit tumor migration and invasion.
Conclusion. Our results demonstrate that Rictor, acting as an oncogene, might be a potential prognostic biomarker and therapeutic target in GC. We suggest that Rictor expression and H. pylori status may be a prognostic marker in gastric cancer.
Abstract
Introduction. Growing evidence indicates that Rictor (Rapamycin-insensitive companion of mTOR) is overexpressed across several malignancies and associated with poor survival. However, only limited data indicate that Rictor plays a role in gastric cancer (GC). We sought to explore the prognostic value of Rictor in GC and present interaction analysis between Rictor expression and H. pylori status regarding their effects over the prognosis of GC patient.
Materials and methods. 250 GC tissues and 124 lymph node metastases were collected for the detection of Rictor by immunohistochemistry. Cox regression model was used to assess the association between Rictor expression and patient prognosis. Functional experiments were examined in transfected cells using Rictor siRNA. Additive and multiplicative interactions of Rictor and H. pylori were evaluated.
Results. In this study, the positive rate of Rictor was 51.6% (129/150) in GC tissues. Multivariate analyses showed that Rictor was independent unfavorable predictor for OS (HR = 1.554, 95% CI = 1.076–2.244, P = 0.019) and DFS (HR = 1.556, 95% CI = 1.081–2.240, P = 0.017). Patients with upregulated Rictor in the primary tumor and lymph node metastases had the worst prognosis. We observed significant additive and multiplicative interactions between Rictor expression and H. pylori status for OS and DFS (P < 0.05). Our in vitro experiment showed that knockdown of Rictor could suppress cell proliferation, induce apoptosis and inhibit tumor migration and invasion.
Conclusion. Our results demonstrate that Rictor, acting as an oncogene, might be a potential prognostic biomarker and therapeutic target in GC. We suggest that Rictor expression and H. pylori status may be a prognostic marker in gastric cancer.
Keywords
gastric cancer; Rictor; H. pylori; prognosis; progression; IHC; siRNA
About this articleTitle
Overexpression of Rictor protein and Rictor-H. pylori interaction has impact on tumor progression and prognosis in patients with gastric cancer
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
96-107
Published online
2020-06-25
Page views
1416
Article views/downloads
869
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2020;58(2):96-107.
Keywords
gastric cancer
Rictor
H. pylori
prognosis
progression
IHC
siRNA
Authors
Fang Wang
Xiaoqi Lou
Yanfeng Zou
Dingtao Hu
Jiatao Liu
Jie Ning
Yang Jiao
Zuoyang Zhang
Feng Yang
Lulu Fan
Hanqing Yu
Wei Wei
Hua Wang
Guoping Sun
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