Rapid colorimetric pyrazinamide susceptibility testing of Mycobacterium tuberculosis
OBJECTIVE: To develop a rapid and reliable colorimetric method for PZA DST using a monotetrazolium redox dye, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), as indicator of viability.
DESIGN: A total of 50 clinical isolates and three standard strains were tested using this colorimetric method and the BACTEC™ MGIT™ 960 system.
RESULTS: Initial test results showed that the PZA DST results were available in 4–6 days; the overall sensitivity and specificity of the CTC colorimetric method were respectively 97.1% and 81.3% in comparison with the MGIT 960 results.
CONCLUSION: These results suggest that the CTC colorimetric method is most rapid among the current PZA DST methods based on culture, and could be used for determining susceptibility to PZA of M. tuberculosis isolates.
Keywords: 5-cyano-2,3-ditolyl tetrazolium chloride; BACTEC MGIT 960 system; PZA; colorimetric methods
Document Type: Research Article
Affiliations: 1: *Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, †University of Chinese Academy of Sciences, Beijing 2: ‡Tuberculosis Reference Laboratory, Hubei Provincial Institute of Tuberculosis Control And Prevention, Wuhan, China 3: *Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan
Publication date: 01 April 2016
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