The structure of the ovary epidermis emitting odorous compounds in Allium karataviense Regel

Beata Żuraw, Elżbieta Weryszko-Chmielewska, Halina Laskowska, Elżbieta Pogroszewska

Abstract


Many plants develop in the petal epidermis papillae emitting essential oils. In species of the genus Allium, papillae occur in the ovary epidermis. The aim of this study was to determine the structure of papillae in Allium karataviense Regel and to perform histochemical tests that would allow the components of their secretion to be investigated, mainly the presence of essential oils. Examination was performed using light and scanning electron microscopy. It was found that in conical papillae, with a height of about 83 μm, there were large cell nuclei, a thick outer wall with a layer of striated cuticle, and a varying degree of cell vacuolation, depending on the age of the ovary. Using histochemical reactions, we demonstrated neutral lipids, acid lipids and pectins to occur in the papillae. Neutral lipids are characteristic of essential oils.

Keywords


Allium L.; ovary; epidermis; micromorphology; structure; histochemistry

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References


Kamenetsky R, Fritsch RM. Ornamental alliums. [In:] Allium Crop Science: recent advances – ed. By Rabinowitch H.D. Currah L., Wallingford, Oxon: CABI Publishing. 2002; 459–491. http://dx.doi.org/10.1079/9780851995106.0459

Krzymińska A. Fenologia i morfologia wybranych gatunków ozdobnych czosnku (Allium L.). Zesz Prob Post Nauk Roln. 2003; 491: 161–168. (in Polish)

Kamenetsky R, Rabinowitch HD. Florogenesis. [In:] Allium Crop Science: recent advances – ed. By Rabinowitch H.D. Currah L., Wallingford, Oxon: CABI Publishing. 2002; 459–491. http://dx.doi.org/10.1079/9780851995106.0031

Krause J. Kwitnące cebule. Ozdobne rośliny cebulowe i bulwiaste. Polski Związek Działkowców. Warszawa. 1992. (in Polish)

Osvald Z. Leksykon roślin cebulowych. Oficyna Wydawnicza MULTICO. Warszawa. 1992. (in Polish)

Świdzińska M (red.). Rośliny kwiatowe 2. Wyd. MUZA, Warszawa. 1998. (in Polish)

Żuraw B. Nektarowanie kwiatów ozdobnych gatunków czosnku z podrodzaju Allium i Melanocrommyum. Mat.

z XLV Nauk. Konf. Pszczel., 11–12 marca 2008, Puławy. 2008: 153–154. (in Polish)

Lipiński M. Pożytki pszczele, zapylanie i miododajność roślin. PWRiL, Warszawa; Wydawnictwo Sądecki Bartnik, Stróże. 2010. (in Polish)

Banaszak J. Pszczoły i zapylanie roślin. Państwowe Wydawnictwo Rolnicze i Leśne, Poznań. 1987. (in Polish)

Vogel S. The role of scent glands in pollination on the structure and function of osmophores. A Merind Publishing Co., New Dehli, India. 1990.

Harborne JB. Ekologia biochemiczna. PWN, Warszawa. 1997. (in Polish)

Esau K. Anatomia roślin. PWRiL, Warszawa. 1973. (in Polish)

Evert RF. Esau’s Plant Anatomy, Third Edition. John Wiley & Sons. Inc. 2006.

Żuraw B, Weryszko-Chmielewska E, Laskowska H, Pogroszewska E. The structure of septal nectaries and nectar presentation in the flowers of Allium aflatunense B. Fedtsch. Acta Agrobot. 2009; 62(2): 31–41. http://dx.doi.org/10.5586/aa.2009.024

Żuraw B, Weryszko-Chmielewska E, Laskowska H, Pogroszewska E. The location of nectaries and nectar secretion in the flowers of Allium giganteum Regel. Acta Agrobot. 2010; 64(2): 33–40. http://dx.doi.org/10.5586/aa.2009.024

Broda B. Metody histochemii roślinnej. PZWL. Warszawa 1971. (in Polish)

Brundrett MC, Kendrick B, Peterson CA. Efficient lipid staining in plant material with Sudan Red 7B or Fluoral Yellow 088 in polyethylene glycol-glycerol. Biotechnic and Histochemistry. 1991; 66: 111–116. http://dx.doi.org/10.3109/10520299109110562

Lison L. Histochemie et cytochemie animals. Principes et mèthods, v.1, 2. Gauthier-Villars, Paris. 1960

Cain AJ. The use of Nile blue in the examination of lipids. Quarterly J Microscopy Sci. 1947; 88: 383–392.

Johansen DA. Plant microtechnique. New York, London: McGraw-Hill. 1940.

Szafranek J, Szafranek B. Substancje powierzchniowe roślin i ich rola w oddziaływaniach roślina – owad. [In:] Biochemiczne oddziaływania środowiskowe. W. Oleszek, K. Głowniak, B. Leszczyński (ed.). Akademia Medyczna, Lublin. 2001; 231–249. (in Polish)

Seigler DS. Role of lipids in plant resistance to insects. [In:] Plant Resistance to Insects. ACS Symposium Series, No. 208, P.A. Hedin (ed.), American Chemical Society, Washington, USA. 1983; 303–327. http://dx.doi.org/10.1021/bk-1983-0208.ch018

Weberling F. Morphology of flowers and inflorescences. Cambridge Univ. Press, Cambridge. 1992.

Stpiczyńska M. Anatomy and ultrastructure of osmophores of Cymbidium tracyanum Rolfe (Orchidaceae). Acta Soc Bot Pol. 1993; 62: 5–9. http://dx.doi.org/10.5586/asbp.1993.001

Stpiczyńska M. Osmophores of the fragrant orchid Gymnadenia conopsea L. (Orchidaceae). Acta Soc Bot Pol. 2001; 70: 91–96. http://dx.doi.org/10.5586/asbp.2001.012

Weryszko-Chmielewska E, Chwil M. Ecological adaptations of the floral structures of Galanthus nivalis L. Acta Agrobot. 2010; 63 (2): 41–49. http://dx.doi.org/10.5586/aa.2010.031

Weryszko-Chmielewska E, Chwil M. Structure of the floral parts of Crocus vernus (L.) Hill. Acta Agrobot. 2011; 64 (4): 35–46. http://dx.doi.org/10.5586/aa.2011.044

Weryszko-Chmielewska E, Sulborska A. Diversity in the structure of the petal epidermis emitting odorous compounds in Viola ×wittrockiana Gams. Acta Sci Pol, Hortorum Cultus. 2012; 11(6): 155–167.

Sulborska A, Weryszko-Chmielewska E, Chwil M. Micromorphology of Rosa rugosa Thunb. Petal epidermis secreting fragrant substances. Acta Agrobot. 2012; 65 (4): 21–28. http://dx.doi.org/10.5586/aa.2012.018

Bergougnoux V, Caissard J-C, Jullien F, Magnard J-L, Scalliet G, Cock JM, Hugueney P, Baudino S. Both the adaxial and abaxial epidermis layers of the rose petal emit volatile scent compounds. Planta. 2007; 226 (4): 853–866. http://dx.doi.org/10.1007/s00425-007-0531-1

Haratym W, Weryszko-Chmielewska E. The ecological features of flowers and inflorescences of two species of the genus Petasites Miller (Asteraceae). Acta Agrobot. 2012; 65(2): 37–46. http://dx.doi.org/10.5586/aa.2012.056

Weryszko-Chmielewska E, Chwil M, Sawidis T. Micromorphology and histochemical traits of staminal osmophores in Asphodelus aestivus Brot. flower. Acta Agrobot. 2007; 60(1): 13–23. http://dx.doi.org/10.5586/aa.2007.002

Sawidis T, Weryszko-Chmielewska E, Anastasiou V, Bosabalidis AM. The secretory glands of Asphodelus aestivus flower. Biologia. 2008; 63: 1118–1123. http://dx.doi.org/10.2478/s11756-008-0151-7

Ventrella MC, Marinho CR. Morphology and histochemistry of glandular trichomes of Cordia verbenacea DC. (Boraginaceae) leaves. Revista Brasil Bot. 2008; 31(3): 457–467. http://dx.doi.org/10.1590/S0100-84042008000300010

Corsi G, Bottega S. Glandular hairs of Salvia officinalis: new data on morphology, localization and histochemistry in relation to function. Ann Bot. 1999; 84: 657–664. http://www.idealibrary.com http://dx.doi.org/10.1006/anbo.1999.0961

Marin M, Ascensao L, Lakušić B. Trichomes of Satureja horvatii Šilić (Lamiaceae) – micromorphology and histochemistry. Arch Biol Sci. Belgrade. 2012; 64(3): 995–1000. http://dx.doi.org/10.2298/ABS1203995M

Whitney HM, Chittka L, Bruce TJA, Glover BJ. Conical epidermal cells allow bees to grip flowers and increase foraging efficiency. Burr Biol. 2009; 19: 948–953. http://dx.doi.org/10.1016/j.cub.2009.04.051

Whitney HM, Bennett KMV, Dorling M, Sandbach L, Prince D, Chittka L, Glover BJ. Why do so many petals have conical epidermis cells? Ann Bot. 2011; 108: 609–616. http://dx.doi.org/10.1093/aob/mcr065




DOI: https://doi.org/10.5586/aa.2013.047

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