Endothelial Lipase Exerts its Anti-Atherogenic Effect through Increased Catabolism of β-VLDLs

Haizhao Yan; Manabu Niimi; Chuan Wang; Yajie Chen; Huanjin Zhou; Fumikazu Matsuhisa; Kazutoshi Nishijima; Shuji Kitajima; Bo Zhang; Hiroshi Yokomichi; Katsuyuki Nakajima; Masami Murakami; Jifeng Zhang; Y. Eugene Chen; Jianglin Fan

doi:10.5551/jat.55244

Original Article

Endothelial Lipase Exerts its Anti-Atherogenic Effect through Increased Catabolism of β-VLDLs

Haizhao Yan, Manabu Niimi, Chuan Wang, Yajie Chen, Huanjin Zhou, Fumikazu Matsuhisa, Kazutoshi Nishijima, Shuji Kitajima, Bo Zhang, Hiroshi Yokomichi, Katsuyuki Nakajima, Masami Murakami, Jifeng Zhang, Y. Eugene Chen, Jianglin Fan

Author information

Haizhao Yan
Department of Molecular Pathology, Faculty of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi
Manabu Niimi
Department of Molecular Pathology, Faculty of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi
Chuan Wang
Department of Molecular Pathology, Faculty of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi
Department of Pharmacology, College of Pharmacy, Shaanxi University of Chinese Medicine
Yajie Chen
Department of Molecular Pathology, Faculty of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi
School of Biotechnology and Health Sciences, Wuyi University
Huanjin Zhou
Department of Molecular Pathology, Faculty of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi
Fumikazu Matsuhisa
Analytical Research Center for Experimental Sciences, Saga University
Kazutoshi Nishijima
Animal Research Laboratory, Bioscience Education-Research Support Center, Akita University
Shuji Kitajima
Analytical Research Center for Experimental Sciences, Saga University
Bo Zhang
Department of Biochemistry, Fukuoka University School of Medicine
Hiroshi Yokomichi
Department of Health Sciences, University of Yamanashi
Katsuyuki Nakajima
Department of Clinical Laboratory Medicine, Graduate School of Medicine, Gunma University
Masami Murakami
Department of Clinical Laboratory Medicine, Graduate School of Medicine, Gunma University
Jifeng Zhang
Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Y. Eugene Chen
Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Jianglin Fan
Department of Molecular Pathology, Faculty of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi
School of Biotechnology and Health Sciences, Wuyi University

Keywords: Endothelial lipase, Hypercholesterolemia, VLDL metabolism, Atherosclerosis, Transgenic rabbits

JOURNAL OPEN ACCESS

2021 Volume 28 Issue 2 Pages 157-168

DOI https://doi.org/10.5551/jat.55244

Browse “Advance online publication” version

Details

Abstract

Aim: Endothelial lipase (EL) plays an important role in lipoprotein metabolism. Our recent study showed that increased hepatic expression of EL attenuates diet-induced hypercholesterolemia, thus subsequently reducing atherosclerosis in transgenic (Tg) rabbits. However, it is yet to be determined whether increased EL activity itself per se is anti-atherogenic or whether the anti-atherogenic effect of EL is exclusively dependent on its lipid-lowering effect.

Methods: To determine the mechanisms underlying EL-mediated anti-atherogenic effect, we fed Tg and non-Tg rabbits diets containing different amounts of cholesterol to make their plasma cholesterol levels similarly high. Sixteen weeks later, we examined their lipoprotein profiles and compared their susceptibility to atherosclerosis.

Results: With Tg and non-Tg rabbits having hypercholesterolemia, the plasma lipids and lipoprotein profiles were observed to be similar, while pathological examinations revealed that lesion areas of both aortic and coronary atherosclerosis of Tg rabbits were not significantly different from non-Tg rabbits. Moreover, Tg rabbits exhibited faster clearance of DiI-labeled β-VLDLs than non-Tg rabbits.

Conclusion: The results of our study suggest that the enhancement of β-VLDL catabolism is the major mechanism for atheroprotective effects of EL in Tg rabbits.

Corresponding author

Register with J-STAGE for free!