Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2013, 157(4):387-391 | DOI: 10.5507/bp.2012.109

Our experience using real-time PCR for the detection of the gene that encodes the superficial lipoprotein LipL32 of the pathogenic leptospires to confirm the acute form of human leptospirosis

Petra Kucerovaa, Zuzana Cermakovaa,b, Lenka Pliskovac, Oto Pavlisb,d, Pavla Kubickovab, Hana Kleprlikovae, Zbynek Valentab,d
a Department of Clinical Microbiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital in Hradec Kralove, Hradec Kralove, Czech Republic
b Central Military Health Institute in Prague, Techonin
c Department of Clinical Biochemistry and Diagnostics, Charles University in Prague, Faculty of Medicine in Hradec Kralove and University Hospital in Hradec Kralove
d Faculty of Military Health Sciences, University of Defence, Hradec Kralove
e Department of Antropology, Faculty of Science, Masaryk University, Brno

Aims: To examine biological materials (blood, urine, cerebrospinal fluid) of patients with suspected leptospirosis using real-time PCR for detecting the gene that codes the superficial LipL32 lipoprotein, and to evaluate the contribution of the real-time PCR method for the laboratory diagnosis of the acute form of leptospirosis.

Material and Methods: During the monitored period of April 2010 - December 2011, a total of 340 biological materials samples were examined (177x blood plasma, 88x urine, 68x, cerebrospinal fluid, 6x bronchoalveolar lavage and 1x sputum) from 216 patients with suspected leptospirosis using real-time PCR LipL32 gene detection.

Results: From the mentioned 216 patients suspected of leptospirosis, 8 patients were evaluated as being PCR LipL32 positive, from which 14 positive biological materials originated (9 x urine, 4x blood and 1x liquor).

Conclusion: As demonstrated in the study, the real-time PCR method for detecting the gene for the superficial lipoprotein LipL32 is an appropriate, quick and reliable method for the diagnosis of the acute form of leptospirosis.

Keywords: leptospirosis, real-time PCR, LipL32, MAT

Received: June 1, 2012; Accepted: December 3, 2012; Prepublished online: December 7, 2012; Published: December 12, 2013  Show citation

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Kucerova, P., Cermakova, Z., Pliskova, L., Pavlis, O., Kubickova, P., Kleprlikova, H., & Valenta, Z. (2013). Our experience using real-time PCR for the detection of the gene that encodes the superficial lipoprotein LipL32 of the pathogenic leptospires to confirm the acute form of human leptospirosis. Biomedical papers, 157(4), 387-391. doi: 10.5507/bp.2012.109
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