Open Access, Peer-reviewed
eISSN 2234-7666
    J Korean Acad Conserv Dent. 2011 Sep;36(5):397-408. Korean.
    Published online Sep 30, 2011.
    https://doi.org/10.5395/JKACD.2011.36.5.397
    Copyright © 2011 Korean Academy of Conservative Dentistry
    Original Article

    Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide

    Yong-Beom Kim, DDS, MSD, PhD,1 Won-Jun Shon, DDS, MSD, PhD, Associate professor ,2 Woocheol Lee, DDS, MSD, PhD, Associate professor ,2 Kee-Yeon Kum, DDS, MSD, PhD, Associate professor ,2 Seung-Ho Baek, DDS, MSD, PhD, Assistant professor ,2 and Kwang-Shik Bae, DDS, MSD, PhD, Professor 2
      • 1Program in Conservative Dentistry, Seoul National University Graduate School, Seoul, Korea.
      • 2Department of Conservative Dentistry, Seoul National University School of Dentistry and Dental Research Institute, Seoul, Korea.
    • Correspondence to Kwang-Shik Bae, DDS, MSD, PhD. Professor, Department of Conservative Dentistry, Seoul National University School of Dentistry, Dental Research Institute, 28-2 Yongon-dong, Jongno-gu, Seoul, Korea 110-749. Tel, +82-2-2072-2651; Fax, +82-2-2072-3859; Email: baeks@snu.ac.kr
    Received July 20, 2011; Revised August 17, 2011; Accepted August 21, 2011.

    Abstract

    Objectives

    This study investigated changes in gene expressions concerning of differentiation, proliferation, mineralization and inflammation using Human-8 expression bead arrays when white Mineral Trioxide Aggregate and calcium hydroxide-containing cement were applied in vitro to human dental pulp cells (HDPCs).

    Materials and Methods

    wMTA (white ProRoot MTA, Dentsply) and Dycal (Dentsply Caulk) in a Teflon tube (inner diameter 10 mm, height 1 mm) were applied to HDPCs. Empty tube-applied HDPCs were used as negative control. Total RNA was extracted at 3, 6, 9 and 24 hr after wMTA and Dycal application. The results of microarray were confirmed by reverse transcriptase polymerase chain reaction.

    Results

    Out of the 24,546 genes, 43 genes (e.g., BMP2, FOSB, THBS1, EDN1, IL11, COL10A1, TUFT1, HMOX1) were up-regulated greater than two-fold and 25 genes (e.g., SMAD6, TIMP2, DCN, SOCS2, CEBPD, KIAA1199) were down-regulated below 50% by wMTA. Two hundred thirty nine genes (e.g., BMP2, BMP6, SMAD6, IL11, FOS, VEGFA, PlGF, HMOX1, SOCS2, CEBPD, KIAA1199) were up-regulated greater than two-fold and 358 genes (e.g., EDN1, FGF) were down-regulated below 50% by Dycal.

    Conclusions

    Both wMTA and Dycal induced changes in gene expressions related with differentiation and proliferation of pulp cells. wMTA induced changes in gene expressions related with mineralization, and Dycal induced those related with angiogenesis. The genes related with inflammation were more expressed by Dycal than by wMTA. It was confirmed that both wMTA and Dycal were able to induce gene expression changes concerned with the pulp repair in different ways.

    Keywords
    Calcium hydroxide; Microarray; Mineral trioxide aggregate; Pulp capping; Pulp cell; Reverse transcriptase polymerase chain reaction

    Figures

    Figure 1
    Classification of expressed genes by wMTA treatment. The genes which were up-or down-regulated by at least two-fold following a cellular treatment with wMTA at any time point were classified using the panther classification system (http://www.pantherdb.org).

    Figure 2
    Classification of expressed genes by Dycal treatment. The genes which were up-or down-regulated by at least two-fold following a cellular treatment with Dycal at any time point were classified using the panther classification system.

    Tables

    Table 1
    Primer sequence list used in RT-PCR

    Table 2
    Summary of expression profiles of genes differentially regulated by wMTA and Dycal

    Table 3
    The relative mRNA expression levels of genes analyzed by RT-PCR

    Notes

    Conflict of Interest: No potential conflict of interest relevant to this article was reported.

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    MeSH Terms
    Aluminum Compounds
    Calcium
    Calcium Compounds
    Calcium Hydroxide
    Dental Pulp
    Dental Pulp Capping
    Drug Combinations
    Gene Expression
    Glutamates
    Guanine
    Humans
    Hydroxides
    Inflammation
    Interleukin-11
    Minerals
    Oxides
    Pemetrexed
    Polytetrafluoroethylene
    RNA
    RNA-Directed DNA Polymerase
    Reverse Transcriptase Polymerase Chain Reaction
    Silicates
    Transcriptome
    Metrics
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