Open Access, Peer-reviewed
eISSN 2234-7666
    J Korean Acad Conserv Dent. 2010 Mar;35(2):96-105. Korean.
    Published online Mar 31, 2010.
    https://doi.org/10.5395/JKACD.2010.35.2.096
    Copyright © 2010 Korean Academy of Conservative Dentistry
    Original Article

    The remineralization aspect of enamel according to change of the degree of saturation of the organic acid buffering solution in pH 5.5

    Jin-Sung Park, Sung-Ho Park, Jeong-Won Park and Chan-Young Lee
      • Department of Dentistry, the Graduate School, Yonsei University, Seoul, Korea.
    • Corresponding Author: Chan-Young Lee. Department of Conservative Dentistry College of Dentistry, Yonsei University, 134 Shinchon-dong, Seodaemun-ku, Seoul, 120-752, Korea. Tel: 82-2-2228-8700, Fax: 82-2-313-7575, Email: chanyoungl@yuhs.ac
    Received December 28, 2009; Revised January 17, 2010; Accepted March 11, 2010.

    Abstract

    The purpose of this study is to observe and compare the remineralization tendencies of artificial enamel caries lesion by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel).

    For this study, 48 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 2 days in order to produce artificial dental caries that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffering remineralization solution of four different degrees of saturation (0.507, 0.394, 0.301, and 0.251) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (×100). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were estimated.

    While the group with the lowest degree of saturation (0.251) showed total remineralization feature from the surface to the subsurface of the lesion, the group with the highest degree of saturation (0.507) showed demineralization mainly on the surface of the lesion at the constant organic acid concentration 0.01 M and pH 5.5.

    Keywords
    Demineralization; Remineralization; pH; Degree of saturation; Artificial cares; Enamel

    Figures

    Figure 1
    Polarizing microscopic observation of demineralized enamel (Group 1, × 100).

    Figure 2
    Polarizing microscopic observation of remineralized enamel (Group 1, × 100).

    Figure 3
    Polarizing microscopic observation of demineralized enamel (Group 2, × 100).

    Figure 4
    Polarizing microscopic observation of remineralized enamel (Group 2, × 100).

    Figure 5
    Polarizing microscopic observation of demineralized enamel (Group 3, × 100).

    Figure 6
    Polarizing microscopic observation of remineralized enamel (Group 3, × 100).

    Figure 7
    Polarizing microscopic observation of demineralized enamel (Group 4, × 100).

    Figure 8
    Polarizing microscopic observation of remineralized enamel (Group 4, × 100).

    Figure 9
    Change ratio of demineralized depth

    (demineralized depth after remineralization / demineralized depth before remineralization) × 100 (%).

    Figure 10
    Change rate of surface lesion width

    (surface lesion width after remineralization / surface lesion width before remineralization) × 100 (%).

    Figure 11
    Comparison of density in enamel area before and after remineralization (Group 1).

    Figure 12
    Comparison of density in enamel area before and after remineralization (Group 2).

    Figure 13
    Comparison of density in enamel area before and after remineralization (Group 3).

    Figure 14
    Comparison of density in enamel area before and after remineralization (Group 4).

    Figure 15
    Change rate of remineralizalized amount before and after remineralization ((demineralized area before remineralization-demineralized area after remineralization/demineralized area before remineralization) ×100 + 100 (%)).

    Tables

    Table 1
    Initial composition of demineralization solution

    Table 2
    Initial composition of remineralization solution

    Table 3
    One-way ANOVA on the remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel

    Table 4
    Demineralization depth (mm) and value change (%) during de- and remineralization

    Table 5
    Surface lesion width (mm) and value change (%) during de-and remineralization

    Table 6
    Remineralization amount (mm2) and value change (%) during de-and remineralization

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    MeSH Terms
    Dental Caries
    Dental Enamel
    Hydrogen-Ion Concentration
    Lactic Acid
    Software
    Tooth
    Metrics
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