Open Access, Peer-reviewed
eISSN 2234-7666
    J Korean Acad Conserv Dent. 2009 Sep;34(5):430-441. Korean.
    Published online Sep 30, 2009.
    https://doi.org/10.5395/JKACD.2009.34.5.430
    Copyright © 2009 The Korean Academy of Conservative Dentistry
    Original Article

    The comparison of gene expression from human dental pulp cells and periodontal ligament cells

    Hyoun So, Sang-Hyuk Park and Gi-Woon Choi
      • Department of Conservative Dentistry, Division of Dentistry, Graduate of Kyung Hee University, Korea.
    • Corresponding Author: Gi-Woon Choi. Department of Conservative Dentistry, Division of Dentistry, Graduate of Kyung Hee University, 1, Hoegi Dong, Dongdaemun Gu, Seoul, Korea, 130-702. Tel: 82-2-958-9336, Email: gwchoi@khu.ac.kr
    Received August 04, 2009; Revised September 03, 2009; Accepted September 04, 2009.

    This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Abstract

    The purpose of this study was to characterize functional distinction between human dental pulp cells(PC) and periodontal ligament cells(PDLC) using cDNA microarray assay and to confirm the results of the microarray assay using RT-PCR. 3 genes out of 51 genes which were found to be more expressed(>2 fold) in PC were selected, and 3 genes out of 19 genes which were found to be more expressed(>2 fold) in PDLC were selected for RT-PCR as well.

    According to this study, the results were as follows:

    1. From the microarray assay, 51 genes were more expressed (2 fold) from PC than PDLC.

    2. RT-PCR confirmed that ITGA4 and TGF β2 were more expressed in PC than in PDLC.

    3. From the microarray assay, 19 genes were more expressed (2 fold) from PDLC than PC.

    4. RT-PCR confirmed that LUM, WISP1, and MMP1 were more expressed in PDLC than in PC.

    From the present study, different expression of the genes between the PC and PDLC were characterized to show the genes which play an important role in dentinogenesis were more expressed from PC than PDLC, while the genes which were related with collagen synthesis were more expressed from PDLC than PC.

    Keywords
    cDNA microarray assay; human dental pulp cells; human periodontal ligament cells; RT-PCR; dentinogenesis; collagen synthesis

    Figures

    Figure 1
    Overview of DNA microarray methology

    Figure 2
    Microarray Scanning Image

    Figure 3
    M-A plot

    The log ratios(M=log2R/G) are plotted on the y-axis against the log of the geometric mean of the signal intensities (A=log2R×G/2). M, expression ratio; A, signal intensity; R, Red for Cy5; G, Green for Cy3.

    Figure 4
    Confirmation of differentially expressed genes observed in microarray results. Three genes (ITGA, NEFH, TGF β2) selected from the genes express more than two fold in pulp cells than periodontal ligament cells were analysed by RT-PCR.

    Figure 5
    Confirmation of differentially expressed genes observed in microarray results. Three genes (MMP1, WISP, LUM) selected from the genes express more than two fold in periodontal ligament cells than pulp cells were analysed by RT-PCR.

    Tables

    Table 1
    Primers for RT-PCR

    Table 2
    The genes expressed more than two fold from the cells cultured with PC than PDLC Ratio = log2 Cy5/Cy3 (Cy5: PDLC, Cy3: PC)

    Table 3
    The genes expressed more than two fold from the cells cultured with PDLC than PC Ratio = log2 Cy5/Cy3 (Cy5: PDLC, Cy3: PC)

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    MeSH Terms
    Collagen
    Dental Pulp
    Dentinogenesis
    Gene Expression
    Humans
    Oligonucleotide Array Sequence Analysis
    Periodontal Ligament
    Metrics
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