Open Access, Peer-reviewed
eISSN 2234-7666
    J Korean Acad Conserv Dent. 2002 May;27(3):257-269. Korean.
    Published online May 31, 2002.
    https://doi.org/10.5395/JKACD.2002.27.3.257
    Copyright © 2002 Korean Academy of Conservative Dentistry
    Original Article

    A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities

    Hyung-Su Kim and Sung-Ho Park
      • Department of Dentistry, The Graduate School of Dentistry, Yonsei University, Korea.

    This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Abstract

    Objective

    The purpose of this study was to evaluate the resin infiltration into dentin of one-bottle adhesive systems and self-etching primer bonded to Class V cavities using confocal laser scanning microscope(CLSM).

    Material and Methods

    Forty Class V cavities were prepared from freshly extracted caries-free human teeth. These teeth were divided into two groups based on the presence of cervical abrasion: Group I, cervical abrasion; Group II, wedge-shaped cavity preparation. Resin-dentin interfaces were produced with two one-bottle dentin bonding systems-ONE COAT BOND(OCB; Coltene®) and Syntac®Srint™(SS; VIVADENT)-, one self-etching priming system-CLEARFIL™ SE BOND(SB; KURARAY)- and one multi-step dentin bonding system-Scotchbond™Multi-Purpose(SBMP, 3M Dental Products)-as control according to manufacturers'instructions. Cavities were restored with Spectrum®(Dentsply). Specimens were immersed in saline for 24 hours and sectioned longitudinally with a low-speed diamond disc. The resin-dentin interfaces were microscopically observed using CLSM. The quality of resin-infiltrated dentin layers were evaluated by five dentists using 0-4 scale.

    Results

    Confocal laser scanning microscopal investigations using primer labeled with rhodamine B showed that the penetration of the primer occurred along the cavity margins.

    Statistical analysis using one-way ANOVA followed by Duncan's Multiple Range test revealed that the primer penetration of the group 2(wedge-shaped cavity preparation) was more effective than group 1(cervical abrasion) and that of the gingival interfaces was more effective than the occlusal interfaces. In the one-bottle dentin bonding systems, the resin penetration score of OCB was compatible to SBMP, but those of SS and self-etching priming system, SB were lower than SBMP.

    Figures

    Fig. 1
    Diagram of longitudinally sectioned tooth showing cavity shapes (shadowed) and schematic orientation of dentinal tubules to the class V cavity surfaces.

    Fig. 2
    Resin penetration scores in occlusal interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

    Fig. 3
    Resin penetration scores in gingival interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

    Fig. 4
    Comparison of resin penetrations of occlusal and gingival interfaces in group 1(cervical abrasion). Asterisk(*) means statistically significant differences (p<0.05)

    Fig. 5
    Comparison of resin penetrations of occlusal and gingival interfaces in group 2(V-shaped cavity preparaion). Asterisk(*) means statistically significant differences(p<0.05)

    Fig. 6.1-6.5
    Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200

    6.1. Score 0., 6.2. Score 1., 6.3. Score 2.,

    6.4. Score 3., 6.5., Score 4.

    Fig. 7.1-7.5
    Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200

    7.1. Score 0., 7.2. Score 1., 7.3. Score 2.,

    7.4. Score 3., 7.5., Score 4.

    Fig. 8
    Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity with Scotchbond multi purpose. magnification × 800 23

    Fig. 9.1-9.4
    Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of cervical abrasion. Original magnification ×200

    Fig. 10.1-10.4
    Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity. Original magnification ×200

    Fig. 11.1-11.4
    Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of cervical abrasion. Original magnification ×200

    Fig. 12.1-12.4
    Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity. Original magnification ×200

    Tables

    Table 1
    Names, numbers and characteristics of each groups of experiment

    Table 2
    Chemical components and instructions for use of the dentin bonding systems used in this study

    Table 3
    Inter-observer correlations of each groups, materials, and parameters

    Table 4
    Resin penetration scores(Mean±SD) at the occlusal interfaces

    Table 5
    Resin penetration scores(Mean±SD) at the gingival interfaces

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    MeSH Terms
    Adhesives
    Dentin
    Dentists
    Diamond
    Humans
    Rhodamines
    Tooth
    Metrics
    Share
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