The landscape of circulating platelet aggregates in COVID-19
Creators
- Nishikawa, Masako1
- Kanno, Hiroshi2
- Yuqi Zhou2
- Xiao, Ting-Hui2
- Suzuki, Takuma3
- Ibayashi, Yuma2
- Harmon, Jeffrey2
- Takizawa, Shigekazu2
- Hiramatsu, Kotaro4
- Nitta, Nao5
- Kameyama, Risako2
- Peterson, Walker2
- Takiguchi, Jun1
- Shifat-E-Rabbi, Mohammad6
- Zhuang, Yan7
- Yin, Xuwang7
- Rubaiyat, Abu Hasnat Mohammad7
- Deng, Yunjie2
- Zhang, Hongqian2
- Miyata, Shigeki8
- Rohde, Gustavo K.9
- Iwasaki, Wataru10
- Yatomi, Yutaka1
- Goda, Keisuke11
- 1. Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan
- 2. Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan
- 3. Department of Computational Biology and Medical Sciences, The University of Tokyo, 277-8562, Japan
- 4. Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan; Research Center for Spectrochemistry, The University of Tokyo, Tokyo 113-0033, Japan
- 5. CYBO, Inc., Tokyo 101-0022, Japan
- 6. Department of Biomedical Engineering, University of Virginia, Virginia 22908, United States
- 7. Department of Electrical and Computer Engineering, University of Virginia, Virginia 22908, United States
- 8. Research and Development Department, Central Blood Institute, Japanese Red Cross Society
- 9. Department of Biomedical Engineering, University of Virginia
- 10. Department of Computational Biology and Medical Sciences, The University of Tokyo, 277-8562, Japan; Department of Biological Sciences, The University of Tokyo, Tokyo 113-0033, Japan; Department of Integrated Biosciences, The University of Tokyo, Tokyo 277-8562, Japan
- 11. Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan; Institute of Technological Sciences, Wuhan University, 430072 Hubei, China; Department of Bioengineering, University of California, Los Angeles, California 90095, United States
Description
A characteristic clinical feature of COVID-19 is the frequent incidence of microvascular thrombosis. In fact, COVID-19 autopsy reports have shown widespread thrombotic microangiopathy characterized by extensive diffuse microthrombi within peripheral capillaries and arterioles in lungs, hearts, and other organs, resulting in multiorgan failure. However, the underlying process of COVID-19-associated microvascular thrombosis remains elusive due to the lack of tools to statistically examine platelet aggregation (i.e., the initiation of microthrombus formation) in detail. Here we report the landscape of circulating platelet aggregates in COVID-19 obtained by massive single-cell image-based profiling and temporal monitoring of the blood of COVID-19 patients (n = 110). Surprisingly, our analysis of the big image data shows the anomalous presence of excessive platelet aggregates in nearly 90% of all COVID-19 patients. Furthermore, results indicate strong links between the concentration of platelet aggregates and the severity, mortality, respiratory condition, and vascular endothelial dysfunction level of COVID-19 patients.
Notes
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