Published September 1, 2021 | Version v1.0
Software Open

Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling -- RNA sequencing and Fiji macro scripts

  • 1. Department of Pathology and Department of Pediatric Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Amalia Children's Hospital, Nijmegen, the Netherlands
  • 2. Department of Pathology and Department of Pediatric Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, the Netherlands
  • 3. Department of Pediatric Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Amalia Children's Hospital, Nijmegen, the Netherlands
  • 4. Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, the Netherlands

Contributors

  • 1. Department of Pathology and Department of Pediatric Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, the Netherlands
  • 2. Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, the Netherlands

Description

This upload contains the R scripts that were used to perform single-cell RNA sequencing analysis and bulk RNA sequencing analysis in the following article:

Jansen, J. et al. Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling (2021). 

Furthermore, the script that was used to automate the macro in Fiji for image quantitation is deposited here. 

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