COVID-19, SARS AND BATS CORONAVIRUSES GENOMES PECULIAR HOMOLOGOUS RNA SEQUENCES

1) 18 RNA fragments of homology equal or more than 80% with human or simian retroviruses have been found in the COVID_19 genome. 2) These fragments are 18 to 30 nucleotides long and therefore have the potential to modify the gene expression of Covid19. We have named them external Informative Elements or EIE. 3) These EIE are not dispersed randomly, but are concentrated in a small part of the COVID_19 genome. 4) Among this part, a 225-nucleotide long region is unique to COVID_19 and Bat RaTG13 and can discriminate and formally distinguish these 2 genomes. 5) In the decreasing slope of the epidemic, this 225 bases area and the 1770 bases Spike region, exhibits an abnormally high rate of mutations/deletions (cases of 44 patients from WA Seattle state, original epicenter in USA). 6) In the comparative analysis of both SPIKES genes of COVID_19 and Bat RaTG13, we note two abnormal facts: • The insertion of 4 contiguous PRRA amino acids in the middle of SPIKE (then we show that this site was already an optimal cleavage site BEFORE this insertion). • An abnormal ratio of synonymous codons / non synonymous codons in the second half of SPIKE. Finally we show the insertion in this 1770 bases SPIKE region of a significant EIE from Plasmodium Yoelii and of a possible HIV1 EIE with a crucial Spike mutation. Through the 14 facts relating to each of the 14 paragraphs of this article, everything converges towards possible laboratory manipulations (End Note below) which contributed to modifications of the genome of COVID_19, but also, very probably much older SARS, with perhaps this double objective of vaccine design and of "gain of function" in terms of penetration of this virus into the cell. This analysis, made in silico, is dedicated to the real authors of Coronavirus COVID_19. It belongs only to them to describe their own experiments and why it turned into a world disaster: 650 000 lives (on 26 July 2020), more than those taken by the two atomic bombs of Hiroshima and Nagasaki. We, the survivors, should take lessons from this serious alert for the future of humanity. We urge our colleagues scientists and medical doctors to respect ethical rules as expressed by Hipocrates oath: do not harm, never and never ! End Note: Why could COVID-19 come from Laboratory manipulations? The following 4 proofs concern differences with respect to SARS either common to COVID-19 and bat RaTG13, or facts radically differentiating these 2 sequences of which it is claimed that the first (COVID-19) comes from a natural evolution of the second (bat RaTG13). We have ranked these 4 proofs in ascending order of importance according to our point of view. 1) Four EIE formally distinguishes COVID-19 and bat RaTG13 genomes from all other SARS or bats genomes. However, their level of HIV/SIV homologies appears much more affirmed for COVID-19 than for bat RaTG13, as if these EIE fragments had recently been “re-injected” into the COVID-19 genome. ==> see & 7, (figures 4 and 5). 2) natural deletions (USA WA Seattle state) apply in priority to EIE inserts (HIV Kenya etc ..). ==> see full Part III and Figure 12 in §13. 3) Synonymous codons mutations within the 1770 bases region of the Spike, which simulate a natural evolution of bat RaTG13 towards COVID-19 while maintaining the optimality obtained in amino acid values, probably from “gain of function” Laboratory experiments (optimality common to both RNA sequences COVID-19 and bat RaTG13) ==> see Figure 10 in & 11 and Figure 11 in §12. 4) “PRRA” amino acids was inserted exactly on the Spike location already theoretically optimal on both COVID-19 and RATG13 (of which it constitutes the main difference). ==> see Figure 13 in & 14.