DAPI and Phase Contrast Images Dataset

Data was acquired on an Olympus IX83 microscope using a 20x/0.4 Ph2 Objective.

Kohler illimination was established before acquistion started.

Sample consists of cultured HeLa Cells on #1.5 (170 um) coverslips, 12mm diameter.

A single coverlsip was imaged in a 10x10 grid with no overlap (total: 100 images)

Each image was acquired sequentially in

• Phase Contrast (50ms exposure time, using a 535nm LED)
• DAPI 100 ms exposure time