ANALYSIS OF CYTOKINES LEVELS IN PATIENTS HAVING HAV DEPENDING ON GENOTYPE AND A PERIOD OF DISEASE DURATION

Introduction. This article is devoted to the research of connection between different viral Hepatitis A genotypes revealed in Ukrainian territory and peculiarities of the main cytokines production.(ФНП а ІЛ-2 ІЛ-4). Matherial & methods. During the dissertation 141 patients having HAV (ВГА) were examined. The medical check-up was conducting  in the next  dynamic:on the inflammation stage and in the early recovering period. Molecular-genetic investigations were carried out with ПЛР(PCR) method. Genotyping was conducting with the help of restrictive fragment length polymorphisms analysis method. In  ІЛ 2 Іл 4 ФНА а researching were applied such testing systems as  ООО Протеиновый контур (Russia St.-Petersburg).Analysis of statistics and processing results were made with the help of the following program  Statistika for Windows(Stat Soft Inc USA). Results & discussion. During cytokines levels research on the inflammation stage of disease depending on ВГА genotype was revealed that the patients with 3 A genotype were marked with significant increase in anti-inflammatory ФНП а  concentration by 1.6(p 0,001) times and regulating Іл 2 by 3.1 (p 0,001) times and decrease anti-inflammatory ІЛ-4 by 2,3 (0,001) times as well in comparison with patients having 1 A genotype. When analysing rank structure of cytokines deviation degree in groups with different genotypes ВГА (HAV) was found out  that  the first rank in 1 А ВГА group possesses increase of ІЛ 2 level  and the last rank in this category has increase of ФНП а. It’s exactly the opposite situation for patients with genotype 3 A  ВГА –they have increase of ФНП а level on the dominate rank position and ІЛ-2 belongs to the last rank category. Total results indicate on the relations between ВГА(HAV) genotype and functional specification of cytokines link. In this case according to the table 1,the potentional difference between 1 A and 3 A lies in ІЛ-4 production. Taking into account that Іл-4 is produced by Tx2 Lymphocytes and ІЛ-2 by Tx1 Lymphocytes there was undertaken the analysis in order to elect the character of Tx1/Tx2  balance in the ratio of Іл-2 /Іл-4 indexes. It implies that the state of cytokines exchanges that patients  with genotype 3 A have shows us the prevailing of Tx2 Lymphocytes response and developing of cell-mediated immune protection defect. Comparisons between cytokines indexes in patients with different genotypes ВГА during the early recovering stage revealed that  patients with 3 A genotype in comparison with ones having  1 A genotype own much higher level of Іл-4 amounts (p 0,005) increased thus by 1.7 times and inaccurate growing of ФНП а (p 0,05) and decrease of Іл-2 (p 0,05). When analysing rank structure of cytokines deviation degree in groups with different genotypes ВГА on the early recovering stage it was revealed that their difference lies in rank positions Іл-2 and Іл-4.If 1 A genotype patients have significant increase of ІЛ-2 amounts and thus it takes the first rank, then decrease of Іл-4 amounts  takes the second rank. According to 3 A genotype patients-they have increase of Іл-4 amounts on the first rank position and increase of Іл-2 takes the second position in the rank position. This represents the existence of specific metabolism of cytokines and ВГА genotype. Prevailing of Tx2 Lymphocytes in 3 A genotype patients unfolds probably the developing of cell-mediated immune protection defect and it also likely acts the role of the unfavourable  ВГА duration predictor. Conclusion. Learning cytokines status in ВГА patients has revealed the essential differences depending on the genotype. Thereby 3 A genotype patients in comparison with 1 A genotype ones have more emphasized (p 0,001) cytokines level deviation from controlling indicators on the inflammation stage. The main features of ЦК homeostasis disbalance is in the following things: 1 A genotype patients have predominance of Іл-2 over Іл-4 and Contrariwise 3 A genotype patients have predominance of Іл-4 over Іл-2.This fact gives evidence of the developing of cell-mediated immune protection defect. 3 A genotype patients in comparison with 1 A genotype patients are identified as having rapid growth of normal amounts of cytokines in blood serum and this leads to the protracted disease stage.