Covered in PubMed, Scopus & SCI
pISSN 2093-2278   eISSN 2093-2286
Open Access, Peer-reviewed
    J Korean Acad Periodontol. 2009 Mar;39(1):77-86. Korean.
    Published online Mar 31, 2009.
    https://doi.org/10.5051/jkape.2009.39.1.77
    Copyright © 2009 Korean Academy of Periodontology
    Original Article

    Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects

    Suk-Hoon Kwon,1 Hyun-Chang Lim,1 Kyung-Hee Choi,2 Min-Soo Kim,1 Ji-Hyun Lee,1 Ui-Won Jung,1 Jeong-Ho Yun,3 Chang-Sung Kim,1 Seong-Ho Choi,1 and Kyoo-Sung Cho1
      • 1Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University, Seoul, Korea.
      • 2Research Development Institute, Cowellmedi Co. LTD, Korea.
      • 3Department of Dentistry, College of Medicine, Kwandong University, Myongji Hospital, Goyang, Korea.
    • Correspondence: Kyoo-Sung Cho. Department of Periodontology, School of Dentistry, Yonsei University, 134 Shin-chon Dong, Seodaemoon-gu, Seoul 120-752, Korea. Email: kscho@yuhs.ac , Tel: 02-2228-3188, Fax: 02-392-0398
    Received February 12, 2009; Accepted March 11, 2009.

    Abstract

    Purpose

    Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system.

    Materials and methods

    Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals.

    Results

    The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks.

    Conclusion

    rhBMP-2 produced by E.coli expression system may be effective for bone regeneration.

    Keywords
    bone morphogenetic protein-2; E.coli expression system; Absorbable collagen sponge; rat calvarial defect

    Figures

    Figure 1
    Schematic diagram of the calvarial osteotomy defect showing the histometric analysis

    Figure 2
    Representative photomicrographs of defect site receiving the sham surgery at 2weeks(arrow heads : defect margin ; HE stain, magnification × 10).

    Figure 3
    Representative photomicrographs of defect site receiving the sham surgery at 8weeks(arrow heads : defect margin ; HE stain, magnification × 10).

    Figure 4
    Representative photomicrographs of defect site receiving the positive control at 2weeks(arrow heads : defect margin ; HE stain, magnification × 10).

    Figure 5
    Representative photomicrographs of defect site receiving the positive control at 8weeks(arrow heads : defect margin ; HE stain, magnification × 10).

    Figure 6
    Representative photomicrographs of defect site receiving the experimental at 2weeks(arrow heads:defect margin ; HE stain, magnification × 10).

    Figure 7
    Representative photomicrographs of defect site receiving the experimental at 2weeks(arrow heads:defect margin, PB:pre-existing bone, NB:new bone ; HE stain, magnification × 100).

    Figure 8
    Representative photomicrographs of defect site receiving the experimental at 2weeks(central portion ; HE stain, magnification × 100)

    Figure 9
    Representative photomicrographs of defect site receiving the experimental at 8weeks(arrow heads: defect margin ; HE stain, magnification × 10).

    Figure 10
    Representative photomicrographs of defect site receiving the experimental at 8weeks(arrow heads:defect margin, PB:pre-existing bone, NB:new bone ; HE stain, magnification × 100).

    Figure 11
    Representative photomicrographs of defect site receiving the experimental at 8weeks(central portin; HE stain, magnification × 100).

    Tables

    Table 1
    Study design

    Table 2
    Defect closure(group means±SD; n=8, %)

    Table 3
    New bone area(group means±SD; n=8, mm2)

    Table 4
    Augmented area(group means±SD; n=8, mm2)

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    MeSH Terms
    Animals
    Bone Regeneration
    Collagen
    Durapatite
    Escherichia
    Escherichia coli
    Humans
    Male
    Osteogenesis
    Rats
    Rats, Sprague-Dawley
    Salicylamides
    Metrics
    Share
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