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Design and Development of a Space Station Cell Culture Unit
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Abstract
To address basic cell biology questions such as “Can cells respond to a gravity stimulus?”, a Cell Culture Unit (CCU) is being developed for use in the International Space Station. The CCU will accommodate diverse specimen types (animal, plant, and microbial cells; tissues; aquatic organisms) in up to twenty-four cell specimen chambers. The environmental conditions (e.g. temperature, pH, gas concentrations) will be maintained by medium recirculation and renewal, and gas and heat exchange. The CCU will also provide for the addition of experimental agents (e.g. growth factors), automated sampling, and monitoring of the specimens by video microscopy. Microgravity experiments will be performed using the CCU within the Habitat Holding Rack (HHR); a CCU within the Space Station Centrifuge will serve as an on-board unit-gravity control.
The CCU design includes a Cell Specimen Environment Assembly to maintain the specimens, a Video Microscope System to obtain specimen images, an Electronics Assembly to control the subsystems and provide signal conditioning, a Power Supply Subsystem to condition power from the HHR or Centrifuge, an Active Cooling Subsystem to maintain specimen temperature, and a Structural Containment Assembly to house the subsystems and interface with the HHR or Centrifuge. Six reference specimens, encompassing the candidates that will likely be flown and that exercise the required ranges of experimental conditions, were selected to test the individual subsystems and the CCU system. Here we report the design and development testing of the first CCU prototypes (e.g. biocompatibility, medium flow and mixing, gas exchange, sampling, video images, cell and tissue culture). Detailed design and testing will continue towards a first launch of the CCU in 2001.
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Citation
Searby, N., de Luis, J., and Vunjak-Novakovic, G., "Design and Development of a Space Station Cell Culture Unit," SAE Technical Paper 981604, 1998, https://doi.org/10.4271/981604.Also In
References
- Hughes-Fulford, M. Lewis, M.L. 1996 Effects of Microgravity on Osteoblast Growth Activation Exp. Cell Research 224 103 109
- Souza, K. Hogan, R. Ballard, R. 1995 Life into Space: Space Life Sciences Experiments NASA Ames Research Center 1965 - 1990 NASA Reference Publication 1372
- Tripathy, B.C. Brown, C.S. Levine H.G. Krikorian A.D. 1996 Growth and Photosynthetic Responses of Wheat Plants Grown in Space Plant Physiology 110 3 801 806
- Bonting, S.L. 1992 Effects of Spaceflight on Growth and Cell Division in higher Plants Advances in Space Biology and Medicine 2 JAI Press Greenwich, Connecticut
- Krikorian, A.D. 1996 Strategies for “Minimal Growth Main-tenance” of Cell Cultures: A Perspective on management for Extended Duration Experimentation in the Microgravity Environment of a Space Station The Botanical Review 62 1 41 108
- De Jong, H. A. Sondag, E.N. Kuipers, A. Oosterveld, W.J. 1996 Swimming behavior of fish during short periods of weightlessness Aviation, Space, and Environmental Medicine 67 463 466
- von Baumgarten, R. J. Simmonds, R. C. Boyd, J. F. Garriott, O. K. 1975 Effects of prolonged weightlessness on the swimming pattern of fish aboard Skylab 3 Aviation, Space, and Environmental Medicine 46 902 906
- Wiederhold, M. L. Gao, W. Y. Harrison, J. L. Hejl, R. 1997 Development of gravity-sensing organs in altered gravity Gravitational and Space Biology Bulletin 10 91 96
- Moorman, S. J Slater, J. Cordova, R. Burress, C. 1997 Development of the zebrafish vestibular system depends on normal gravity Proceedings of the Society for Neurosciences 23
- A Strategy for Space Biology and Medical Science for the 1980s and 1990s National Academy Press Wash, DC 1987
- Cogoli, A. Cogoli-Greuter, M. Morrison, D.R. 1997 Activation and Proliferation of Lymphocytes and other Mammalian Cells in Microgravity Adv. Space Biol. Med. 6 33 79
- Kulesh, D.A. et al. 1994 Space Shuttle flight (STS-45) of L8 myoblast cells results in the isolation of a nonfusing cell line variant J. Cell Biochem 55 4 530 544
- Freed, L.E. Langer, R. Martin, I. Pellis, N.R. Vunjak Novakovic, G 1997 Tissue engineering of cartilage in space Proc. Natl. Acad. Sci. 94 13885 13890
- Levenspiel, O. Chemical Reaction Engineering OSU press, Oregon State University Corvallis, OR 1972