Open Access, Peer-reviewed
pISSN 2288-3886
eISSN 2288-3959
    Korean J Nutr. 2011 Dec;44(6):481-487. Korean.
    Published online Dec 31, 2011.
    https://doi.org/10.4163/kjn.2011.44.6.481
    © 2011 The Korean Nutrition Society
    Original Article

    Relationship between Bone Morphological Microstructure and Inflammatory Markers in Growing Mice Fed a High Fat Diet

    Mi-Sung Kim,1 Hyun-A Lee,2 Ok-Jin Kim,2 and Cheong-Min Sohn1
      • 1Department of Food and Nutrition, Wonkwang University, Iksan 570-749, Korea.
      • 2Center for Animal Resources Development, Wonkwang University, Iksan 570-749, Korea.
    • To whom correspondence should be addressed. (Email: ccha@wku.ac.kr )
    Received October 08, 2011; Revised November 12, 2011; Accepted November 17, 2011.

    Abstract

    Obesity not only reduces bone mineral density but also increases inflammatory markers. Therefore, we examined the change in inflammatory markers and morphological microstructure of the bones using a mouse model fed a high-fat diet. C57BL/6J 4-week-old male mice were divided into a control group (n = 6) and a experimental group (n = 6); the control group was provided with 10% Kcal fat diet, and the high-fat diet group was provided with 45% Kcal fat diet for 12 weeks using the free provision method. Blood was analyzed for inflammatory markers, and micro-computed tomography was used to measure the morphological microstructure of the femoral bone. The weight increases in the control group and high-fat diet group were 5.85 ± 1.84 g and 16.06 ± 5.64 g, respectively (p < 0.01), glucose was 115.00 ± 16.88 mg/dL and 188.33 ± 13.29 mg/dL (p < 0.01), and triglycerides were 65.00 ± 6.19 mg/dL and 103.33 ± 8.02 mg/dL (p < 0.05) respectively. Leptin and interleukin (IL)-6 were significantly higher in the high-fat diet group than that in the control group (p < 0.01). As a result of a biochemical index analysis of bone metabolism, osteocalcin tended to be lower in the high-fat diet group, whereas CTx was significantly higher in the high-fat diet group compared to that in the control group (p < 0.01). The thickness of the bony trabecula was significantly narrower in the high-fat diet group than that in the control group (p < 0.05), and the gap in the bony trabecula was significantly wider in the high-fat diet group than that in the control group (p < 0.05). IL-6 and the gap in the bone trabecula, which was a morphological microstructure of the bones, showed a positive correlation (p < 0.05). Taken together, inducing obesity through a high-fat diet in mice during the growth phase caused a change in bone microstructure and was correlated with the inflammation index. Accordingly, restriction of excessive fat intake may be needed to suppress the inflammatory reactions and promote normal bone formation.

    Keywords
    high fat diet; inflammation markers; bone morphological microstructure

    Figures

    Fig. 1
    Results were gain weight of control and high-fat diet mice.

    Fig. 2
    Micro-CT scout image of control and high-fat diet mice.

    Tables

    Table 1
    Body weight and food efficiency ratio of control and high-fat diet mice

    Table 2
    Body fat weight of control and high-fat diet mice

    Table 3
    Biochemical indices of control and high-fat diet mice

    Table 4
    Bone mineral density and bone morphological microstructure of control and high-fat diet mice

    Table 5
    Correlation of bone mineral density and bone morphological microstructure with inflammatory markers

    Notes

    This work was supported by grants from Wonkwang University in 2011.

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    MeSH Terms
    Animals
    Bone Density
    Diet
    Diet, High-Fat
    Glucose
    Humans
    Inflammation
    Interleukin-6
    Interleukins
    Leptin
    Male
    Mice
    Obesity
    Osteocalcin
    Osteogenesis
    Triglycerides
    Metrics
    Share
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