Anti-atherosclerotic Effect of Green Tea in Poluynsaturated Fatty Acids-treated Apo E KO Mice

Kim, Hyo-Sook; Lee, Myoungsook

doi:10.4163/kjn.2011.44.6.465

Korean J Nutr. 2011 Dec;44(6):465-473. Korean.
Published online Dec 31, 2011.
https://doi.org/10.4163/kjn.2011.44.6.465

Original Article

Anti-atherosclerotic Effect of Green Tea in Poluynsaturated Fatty Acids-treated Apo E KO Mice

Hyo-Sook Kim and Myoungsook Lee

Author information

Author notes

Copyright and License

- Food and Nutrition and Research Institute of Obesity Sciences, Sungshin Women's University, Seoul 136-742, Korea.
To whom correspondence should be addressed. (Email: mlee@sungshin.ac.kr )

Received November 14, 2011; Revised December 06, 2011; Accepted December 14, 2011.

Abstract

Dietary fatty acids are under intense research to identify anti-atherogenic mechanisms, so we investigated green tea powder (GT) as a protector against atherogenesis originating from lipid peroxidation such as 4-hydroxynonemal (4-HNE) and malondialdehyde (MDA) in different dietary fatty acid-treated apo E KO mice. Growth rate and dietary efficiency were lower in apo E KO mice with or without LA compared to wild type. Plasma total cholesterol (TC) and triacylglycerol (TG) did not correspond to values in other tissues, but TG in heart tissue decreased significantly by GT after linoleic acid (LA) or docosahexaenoic acid (DHA) was administered. LA induced apoptosis as evidenced by changes in aorta morphology and immunohistochemistry. Lipid peroxides (LPO) was increased in apo E KO mice with or without LA corresponding to the accumulation of 4-HNE or MDA in the proximal aorta above the atria. GT consumption tended to reduce the primary causal mechanism of atherogenic phenomena such as oxidizability in both LA and DHA treated atherogenic mice. A high polyunsaturated fatty acids (PUFA) diet involved the changes on stress-induced apoptotic signaling by increasing caspase 3, cytochrome c, and nuclear factor-κB in the heart tissue, but decreasing the bcl-2 protein. However, GT remarkably reduced the expression of apoptotic signaling, in contrast to the PUFA diet. Therefore, the potential of GT as an anti-atherosclerotic dietary antioxidant was tested in this study.

Keywords

green tea; antiatherosclerosis; 4-HNE; aorta morphology; apoptosis

Figures

Fig. 1
Research design for this study.

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Fig. 2
A: Gross morphology of proximal aortae in 20%LA, 20%LA + 5%GT, 20%DHA and 20%DHA + 5%GT treated apo E KO mice. The presence of atherosclerotic lesion (white arrow) were clearly observed at inner-curvature and branch points of aorta in apo E KO mice. B: Representative image of an aortic root atherosclerotic lesion (white arrow) in LA, LA-GT, DHA and DHA-GT treated C57BL/6J apoE KO mice.

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Fig. 3
Cell signaling of mitochondrial damage from the hepatocytes of apoE KO mice and 20% LA, 20% LA + 5% GT, 20% DHA and 20% DHA + 5% GT treated apo E KO mice.

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Tables

Table 1
Diet composition of experimental diet

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Table 2
The mean of body weight gains, food intake and food efficiency in apo E KO mice fed PUFA with GT for 8 weeks

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Table 3
Effects of dietary green tea on TC and TG levels of serum, heart and liver in different 20% PUFA fed apo E KO mice

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Table 4
The differences in the total lipid peroxides (LPO) including MDA and 4-HNE of serum, heart and liver in 20% PUFA with GT treated apo E KO mice

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References

1. National Statistics Office. 2008 Annual Report on the cause of Death Statistics. Republics of Korea: 2009.
1. Romos KS, Melchert RB, Chacon E, Acosta D Jr. In: Casarett & Doull's Toxicology. 6th ed. New York: McGraw-Hill; 2011. Toxic responses of the heart and vascular systems; pp. 597-651.
1. Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 1993;362(6423):801–809.
  PubMed
  
  CrossRef
1. Oh H, Mun H, Lee M. Effect of CLA (Conjugated Linoletic Acid) on the Anti-Atherosclerotic Factors in Human Hepatoma HepG2 Cells. Korean J Nutr 2004;37(3):182–192.
1. Natio HK. Atherogenesis Current topics on etiology and risk factors. Clin Chem 1995;41(1):132–113.
1. Kim WS, Kim YS. Paraxonase Polymorphism as a new genetic marker of atherosclerosis. Korean J Lipidol 1996;6(2):81–86.
1. Jang YS, Kim OY, Kwon SJ, Lee JH, Chung NS, Kwon SJ, Huh KB. Influence of alcohol consumption and smoking habits on cardiovascular risk factors and antioxidant status in healthy men. Korean J Med 1999;56(4):437–449.
1. Witztum JL, Steinberg D. Role of oxidised low density lipoprotein in atherogenesis. J Clin Invest 1991;88(6):1785–1792.
1. Kris-Etherton PM, Krummel D, Russell ME, Dreon D, Mackey S, Borchers J, Wood PD. The effect of diet on plasma lipids, lipoproteins, and coronary heart disease. J Am Diet Assoc 1988;88(11):1373–1400.
1. Park BS, Response surface. n-6 and P/S ratio on reduction of plasma lipids in rats. J Korean Oil Chem Soc 2004;21(2):148–155.
1. Levy RI. Choesterol, lipoproteins, apoproteins and hearts disease: present status and future prospects. Clin Chem 1981;27(5):653–662.
1. Dyerberg J, Bang HO, Stofferson E, Moncada S, Vane JR. Eicosapentaenoic acid and prevention of thrombosis and atherosclerosis? Lancet 1978;2(8081):117–119.
1. Hu ML, Frankel EN, Leibovitz BE, Tappel AL. Effect of dietary lipids and vitamin E on in vitro lipid eroxidation in rat liver and kidney homogenates. J Nutr 1989;119(11):1574–1582.
1. Buckingham KW. Effect of dietary polyunsaturated/saturated fatty acid ratio and dietary vitamin E on lipid peroxidation in the rat. J Nutr 1985;115(11):1425–1435.
1. Staprans I, Rapp JH, Pan XM, Feingold KR. The effect of oxidized lipids in the diet on serum lipoprotein peroxides in control and diabetic rats. J Clin Invest 1993;92(2):638–643.
1. Fujiki H. Two stages of cancer prevention with green tea. J Cancer Res Clin Oncol 1999;125(11):589–597.
1. Arts IC, Hollman PC, Feskens EJ, Bueno de Mesquita HB, Kromhout D. Catechin intake might explain the inverse relation between tea consumption and ischemic heart disease: the Zutphen Elderly Study. Am J Clin Nutr 2001;74(2):227–232.
1. Yang TT, Koo MW. Inhibitory effect of Chinese green tea on endothelial cell-induced LDL oxidation. Atherosclerosis 2000;148(1):67–73.
1. Miura Y, Chiba T, Tomita I, Koizumi H, Miura S, Umegaki K, Hara Y, Ikeda M, Tomita T. Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice. J Nutr 2001;131(1):27–32.
1. Yang CS, Wang ZY. Tea and cancer. J Natl Cancer Inst 1994;85(13):1038–1049.
1. Suganuma M, Okabe S, Sueoka N, Sueoka E, Matsuyama S, Imai K, Nakachi K, Fujiki H. Green tea and cancer chemoprevention. Mutat Res 1999;428(1-2):339–344.
1. Kim SO, Lee MY. Effects of Ethylacetate Fraction of Onion on Lipid Metabolism in High Cholesterol-Fed Rats. J Korean Soc Food Sci Nutr 2001;30(4):673–678.
1. Miyazaki, Miwa S, Kodama H, Yamada H, Nagata K, Toriumi W, Kitamura K, Kume E. Hepatic and intestinal changes in rats treated with T-0126, a microsomal triglyceride transfer protein (mtp) inhibitor. J Toxicol Sci 2007;32(2):161–177.
1. Chang CY, Chen YL, Yang SC, Huang GC, Tsi D, Huang CC, Chen JR, Li JS. Effect of schisandrin B and sesamin mixture on CCl(4)-induced hepatic oxidative stress in rats. Phytother Res 2009;23(2):251–256.
1. Kim JS, Kim SH, Han YN. Effects of Unsaturated Fatty Acid Diets and Feeding Periods on the Antithrombosis, the Hematological Changes in the Blood and Fatty Acid Compositions of Platelets in Rats. Korean J Nutr 1992;25(5):339–350.
1. Bulliyya G, Reddy KK, Reddy GP, Reddy PC, Reddanna P, Kumari KS. Lipid profiles among fish-consuming coastal and non-fish-consuming inland populations. Eur J Clin Nutr 1990;44(6):481–485.
1. Tonooka F, Matsumoto N, Ishigaki A, Hara Y. The effects of crude catechins of tea on the food in take and body fat in rat; Proceeding of International Symposium Tea Science; 1991. 336 p.
1. Jin HH, Yang JL, Chung JH, Kim Y. Hypocholesterolemic effects of green tea in cholesterol-fed rats. J Korean Soc Food Sci Nutr 2004;33(1):47–51.
1. Kim ES, Kim MK. Effect of dried leaf powders and ethanol extracts of persimmon, green tea and pine needle on lipid metabolism and antioxidative capacity in rats. Korean J Nutr 1999;32(4):337–352.
1. Nestel PJ, Havenstein N, Whyte HM, Scott TJ, Cook LJ. Lowering of plasma cholesterol and enhanced sterol excretion with the consumption of polyunsaturated ruminant fats. N Engl J Med 1973;288(8):379–382.
1. Kwon MN, Yang JL, Chung JH, Kim YH. Effect of flavonoid (+)-catechin as stabilizer in rat fed fresh and peroxidized fish oil. J Korean Food Nutr 1993;22(4):381–391.
1. Matsuda H, Chisaka T, Kubomura Y, Yamahara J, Sawada T, Fujimura H, Kimura H. Effects of crude drugs on experimental hypercholesterolemia. I. Tea and its active principles. J Ethnopharmacol 1986;17(3):213–224.
1. Vahouny GV, Khalafi R, Satchithanandam S, Watkins DW, Story JA, Cassidy MM, Kritchevsky D. Dietary fiber supplementation and fecal bile acids, neutral steroids and divalent cations in rats. J Nutr 1987;117(12):2009–2015.
1. Cho SH, Choi YS. Lipid peroxidation and antioxidant status is affected by different vitamin E levels when feeding fish oil. Lipids 1994;29(1):47–52.
1. Choi YS, Cho SH, Im JK. Lipid peroxidation and vitamins e and a levels in tissues of rats fed fish oil or soybean oil supplemented with vitamin E. Korean J Nutr 1995;28(10):967–975.
1. Jorgensen KA, Dyerberg K. Prostacyclin, thromboxane and atherosclerosis. In: Draper HH, editor. Advances in Nutritional Research. 1983. pp. 64-66.
1. Tijburg LB, Wiseman SA, Meijer GW, Weststrate JA. Effects of green tea, black tea and dietary lipophilic antioxidants on LDL oxidizability and atherosclerosis in hypercholesterolaemic rabbits. Atherosclerosis 1997;135(1):37–47.
1. Faraco PR, Ledgerwood EC, Vandenabeele P, Prins JB, Bradley JR. Tumor necrosis factor induces distinct patterns of caspase activation in WEHI-164 Cells associated with apoptosis or necrosis depending on cell cycle stage. Biochem Biophys Res Commun 1999;261(2):385–392.
1. Chen ZY, Istfan NW. Docosahexaenoic acid is a potent inducer of apoptosis in HT-29 colon cancer cells. Prostaglandins Leukot Essent Fatty Acids 2000;63(5):301–308.
1. Diep QN, Intengan HD, Schiffrin EL. Endothelin-1 attenuates omega3 fatty acid -induced apoptosis by inhibition of caspase 3. Hypertension 2000;35(1 Pt 2):287–291.