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    Korean J Urol. 2008 Mar;49(3):266-270. Korean.
    Published online Mar 31, 2008.
    https://doi.org/10.4111/kju.2008.49.3.266
    Copyright © 2008 The Korean Urological Association
    Original Article

    Electroejaculation in the Male Rat

    Sung Hyun Paick, Byung Ki Lee, Sung Wook Yoon, Minki Baek, Hyeong Gon Kim, Eun Young Song,1 Young Soo Lyoo,2 and Yong Soo Lho
      • Department of Urology, College of Medicine, Konkuk University, Seoul, Korea.
      • 1Department of Laboratory Medicine, College of Medicine, Konkuk University, Seoul, Korea.
      • 2Department of Veterinary Pathology, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
    Received October 25, 2007; Accepted January 19, 2008.

    Abstract

    Purpose

    We examined the possibility of electroejaculation for the evaluation of fertility in the male rat.

    Materials and Methods

    Ten 8-week-old Sprague-Dawley male rats that underwent seminal vesiculectomy were used for semen collection by electroejaculation. With a transrectal probe (60Hz, 3V, 0.5A), sign wave electric stimulation was applied to an anesthetized rat. Ejaculated semen was collected by a pipette and the volume and sperm density of the ejaculate were analyzed. Two weeks later, a second electroejaculation was performed under the same conditions with the same rats.

    Results

    At the first attempt, all 10 rats ejaculated following electric stimulation. The mean semen volume was 8.9µl (range, 3.0-28.5µl) and the mean sperm density was 6,428/ml (range, 320-20,997/ml). At the second attempt, only 7 of 10 rats (70%) ejaculated. The mean semen volume was 5.6µl (range, 3.3-8.6µl) and the mean sperm density was 2,801/ml (range, 227-12,555/ml).

    Conclusions

    These results indicated that electroejaculation has the possibility of being a useful method for evaluation of fertility in the male rat.

    Keywords
    Electric stimulation; Ejaculation; Fertility; Rats

    Figures

    Fig. 1
    The transrectal probe (hand-made) for electroejaculation in the male rat.

    Fig. 2
    The electrical stimulator for electroejaculation in the male rat.

    Fig. 3
    Changes of sperm density for the first and second attempts for electroejaculation.

    Tables

    Table 1
    Volume and sperm concentration of semen obtained with electroejaculation

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    MeSH Terms
    Animals
    Ejaculation
    Electric Stimulation
    Fertility
    Humans
    Male
    Rats
    Semen
    Spermatozoa
    Metrics
    Share
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